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Marine Biology

, Volume 38, Issue 2, pp 101–109 | Cite as

Inorganic nitrogen metabolism in marine bacteria: The intracellular free amino acid pools of a marine pseudomonad

  • S. O. Stanley
  • C. M. Brown
Article

Abstract

The marine pseudomonad bacterium PL1 contains an intracellular pool of free amino acids which consist mainly of glutamate with small amounts of glutamine and aspartate when grown in a nutrient medium containing 0.2 M NaCl. When the NaCl concentration of the growth medium is increased to 0.8 M, proline becomes a major component of the intracellular pool together with glutamate—at this molarity and under suitable nutrient conditions these amino acids comprise 20% of total bacterial amino acid nitrogen. When grown in a nutrient growth medium containing a constant level of NaCl, the intracellular pool size can vary by a factor of 4 depending on the concentration of carbon and nitrogen in the medium. Experiments show that the amino acid pool can act as a nitrogen reserve but has little function as a carbon reserve. At high NaCl concentrations there is a marked dependence for growth on the presence of sufficient potassium in the medium. However, no correlation between K+ and glutamate concentration in either nitrogen or K+-limited cultures has been found. None of the enzymes associated with glutamate biosynthesis was influenced by NaCl levels between 0.2 and 0.5 M. Neither Na+ or K+ stimulated the activity of these enzymes when tested in vitro.

Keywords

Glutamate Free Amino Acid Intracellular Pool Amino Acid Pool Carbon Reserve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • S. O. Stanley
    • 1
  • C. M. Brown
    • 2
  1. 1.Dunstaffnage Marine Research LaboratoryObanScotland
  2. 2.Department of Biological SciencesUniversity of DundeeDundeeScotland

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