Abstract
Digitonin extracts from retinas of the marine fish Porichthys notatus, from California and Puget Sound (USA), contain a single photopigment with maximum absorbance at 498 nm. In the presence of hydroxylamine the final product of bleaching absorbs maximally at 367 nm, indicating that the photopigment is based on retinal. Spectral sensitivity measurements performed on intact eyes and eye-cup preparations in darkadapted and selectively light-adapted states yield curves which suggest that the retina of P. notatus contains at least 3 different types of receptors. In the darkadapted state, the spectral sensitivity curve has a broad peak, with a maximum between 480 and 500 nm. In the blue and red light-adapted states, in addition to depressing the sensitivity, the shape of the sensitivity curve also changes. Under these conditions new peaks appear which suggest the presence of at least two different types of cones, with sensitivity maxima in the blue and the green regions of the spectrum, respectively. Comparison of the spectral sensitivity curves with the in vivo bioluminescence emission spectrum of the fish gives an especially good correspondence and suggests that the fish is able to perceive the bioluminescence of its own species.
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Literature Cited
Barnes, A.T., J.F. Case and F.I. Tsuji: Induction of bioluminescence in a luciferin deficient form of the marine teleost, Porichthys, in response to exogenous luciferin. Comp. Biochem. Physiol. 46A, 709–723 (1973)
Boden, B.P. and E.M. Kampa: Spectral composition of the luminescence of the euphausiid Thysanoessa raschii. Nature, Lond. 184, 1321–1322 (1959)
— and B.C. Abbott: Photoreception of a planktonic crustacean in relation to light penetration in the sea. In: Progress in photobiology, Proceedings of the Third International Congress on Photobiology, pp 189–196. Ed. by B.C. Christensen and B. Buchmann, Amsterdam: Elsevier Publishing Co. 1961
Clarke, W.D.: Function of bioluminescence in mesopelagic organisms. Nature, Lond. 198, 1244–1246 (1963)
Crane, J.M., Jr.: Bioluminescent courtship display in the teleost Porichthys notatus. Copeia 1965, 239–241 (1965)
Dartnall, H.J.A.: The interpretation of spectral sensitivity curves. Br. med. Bull. 9, 24–30 (1953)
Denton, E.J., J.B. Gilpin-Brown and P.G. Wright: On the ‘filters’ in the photophores of mesopelagic fish and on a fish emitting red light and especially sensitive to red light. J. Physiol., Lond. 208, 72–73 (1970)
Fernandez, H.R.: Spectral sensitivity and visual pigment of the compound eye of the galatheid crab Pleuroncodes planipes. Mar. Biol. 20, 148–153 (1973)
Fraser, J.: Nature adrift, 178 pp. London: G.T. Foulis & Company 1962
Harvey, E.N.: Bioluminescence, 649 pp. New York: Academic Press 1952
Hastings, J.W.: Light to hide by: ventral luminescence to camouflage the silhouette. Science, N.Y. 173, 1016–1017 (1971)
Ibara, R.M.: The biology of the northern midshipman fish Porichthys notatus Girard, M.A. Thesis, University of California, Santa Barbara 1967
Ibara, R.M.: The buoyancy change in the diel vertically migrating fish, Porichthys notatus, Ph.D. Thesis, University of California, Santa Barbara 1970
Jerzmańska, A.: The structure and the biological significance of light-organs in Teleostei. Przegl. zool. 4, 112–118 (1960)
Kampa, E.M. and B.P. Boden: Light generation in a sonic-scattering layer. Deep-Sea Res. 4, 73–92 (1956)
Lane, E.E.: A study of the Atlantic midshipman, Porichthys porosissimus, in the vicinity of Port Aransas, Texas. Contr. mar. Sci. 12, 1–53 (1967)
Lawry, J.V., Jr.: Lantern fish compare downwelling light and bioluminescence. Nature, Lond. 247, 155–157 (1974)
Nicol, J.A.C.: Observations on luminescence in pelagic animals. J. mar. biol. Ass. U.K. 37, 705–752 (1958)
O'Day, W.T. and H.R. Fernandez: Aristostomias scintillans (Malacosteidae): a deep-sea fish with visual pigments apparently adapted to its own bioluminescence. Vision Res. 14, 545–550 (1974)
Springer, V.G.: Mysterious midshipman ... little known curiosity of the sea. Tex. Game Fish 1957, 6–7 (1957)
Tsuji, F.I., A.T. Barnes and J.F. Case: Bioluminescence in the marine teleost, Porichthys notatus, and its induction in a non-luminous form by Cypridina (ostracod) luciferin. Nature, Lond. 237, 515–516 (1972)
—, Y. Haneda, R.V. Lynch, III and N. Sugiyama: Luminescence cross-reactions of Porichthys luciferin and theories on the origin of luciferin in some shallow-water fishes. Comp. Biochem. Physiol. 40A, 163–179 (1971)
Tsuji, F.I., B.G. Nafpaktitis, T. Goto, M.J. Cormier, J.E. Wampler and J.M. Anderson: Spectral characteristics of the bioluminescence induced in the marine fish, Porichthys notatus, by Cypridina (ostracod) luciferin. Molec. cell. Biochem. (1975). (In press)
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Communicated by J.S. Pearse, Santa Cruz
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Fernandez, H.R., Tsuji, F.I. Photopigment and spectral sensitivity in the bioluminescent fish Porichthys notatus . Marine Biology 34, 101–107 (1976). https://doi.org/10.1007/BF00390751
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DOI: https://doi.org/10.1007/BF00390751