Abstract
The unsaturated isoprenoid alcohol phytol is a constituent of the major chlorophyll species a and b and, as such, should be useful as an estimator for chlorophyll values. A method for the extraction of phytol from estuarine suspended organic matter and analysis by gas-liquid chromatography (GLC) is described. Comparisons of the GLC method for phytol-chlorophyll analyses and a spectrophotometric chlorophyll method indicate that the GLC method gives values in good agreement with the spectrophotometric procedure. Similar trends in suspended chlorophyll values were observed in Narragansett Bay (Rhode Island, USA) water samples using the GLC method and a fluorometric procedure. The results of these studies indicate that the GLC method provides reliable analyses of chlorophyll and, although more time-consuming, has the advantage that other organic species can be analyzed in the same sample, thus providing additional information on its chemical composition.
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Literature Cited
Daley, R.J., C.B.J. Gray and S.R. Brown: A quantitative, semiroutine method for determining algal and sedimentary chlorophyll derivatives. J. Fish. Res. Bd Can. 30, 345–356 (1973)
Dehadri, P.V. and R.M.S. Bhargava: Distribution of chlorophyll, carotenoids, and phytoplankton in relation to certain environmental factors along the Central West Coast of India. Mar. Biol. 17, 30–37 (1972)
Humphrey, G.F.: The concentration of chlorophylls a and c in the south-west Pacific Ocean. Aust. J. mar. Freshwat. Res. 21, 1–10 (1970)
Jeffries, H.P.: Fatty-acid ecology of a tidal marsh. Limnol. Oceanogr. 17, 433–440 (1972)
Kawarada, Y. and A. Sano: Distribution of chlorophyll and phaeophytin in the western Pacific Ocean. Oceanogr. Mag. 21, 137–146 (1969)
Lorenzen, C.J.: A method for the continuous measurement of in vivo chlorophyll concentration. Deep Sea Res. 13, 223–227 (1966)
Nissenbaum, A., M.J. Baedecker and I.R. Kaplan: Organic geochemistry of Dead Sea sediments. Geochim. cosmochim. Acta 36, 709–727 (1972)
Patton, S. and A.A. Benson: Phytol metabolism in the bovine. Biochim. biophys. Acta 125, 22–32 (1966)
Pratt, D.M.: The winter-spring diatom flowering in Narragansett Bay. Limnol. Oceanogr. 10, 173–184 (1965)
Schultz, D.M. and J.G. Quinn: Fatty acid composition of organic detritus from Spartina alterniflora. J. Estuar. cstl mar. Sci. 1, 177–192 (1973)
Sever, J. and O.L. Parker: Fatty alcohols (normal and isoprenoid) in sediments. Science, N.Y. 164, 1052–1054 (1969)
U.N.E.S.C.O.: Determination of photosynthetic pigments in sea water. In: Monographs on oceanic methodology, pp 11–18. Montreaux: Corbaz 1966
Zsolnay, A.: Hydrocarbon and chlorophyll: a correlation in the upwelling region off West Africa. Deep Sea Res. 20, 923–925 (1973)
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Communicated by O. Kinne, Hamburg
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Schultz, D.M., Quinn, J.G. Measurements of phytol in estuarine suspended organic matter. Mar. Biol. 27, 143–146 (1974). https://doi.org/10.1007/BF00389067
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DOI: https://doi.org/10.1007/BF00389067