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Marine Biology

, Volume 51, Issue 1, pp 69–76 | Cite as

Epifluorescence microscopy, a technique for the study of feeding in Crassostrea virginica veliger larvae

  • J. Babinchak
  • R. Ukeles
Article

Abstract

Epifluorescence microscopy was applied as a new technique to observe the response of Crassostrea virginica veliger larvae to the algal foods Monochrysis lutheri or Chlorella autotrophica. Uptake, lysis, and digestion or rejection of these algae by oyster larvae were observed microscopically through the autofluorescence of chlorophyll a and its derivatives. Both species of algae were taken up by large numbers of larvae within 5 min after being added to larval cultures. The good growth of larvae obtained with M. lutheri as a food source was related to rapid uptake, lysis, and digestion of this alga. Larvae fed with C. autotrophica did not grow. Althogh C. autotrophica was also taken up rapidly by the larvae, there was no lysis or digestion of these algae and cells were eventually voided.

Keywords

Chlorella Epifluorescence Microscopy Crassostrea Virginica Algal Food Larval Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature Cited

  1. Cole, H.A.: Experiments in the breeding of oysters (Ostrea edulis) in tanks, with special reference to the food of the larva and spat. Fishery Invest., Lond. (Ser. 2) 15, 1–28 (1937)Google Scholar
  2. Davis, H.C.: On food and feeding of larvae of the American oyster, C. virginica. Biol. Bull. mar. biol. Lab., Woods Hole 104, 334–350 (1953)CrossRefGoogle Scholar
  3. — and R.R. Guillard: Relative value of ten genera of micro-organisms as foods for oyster and clam larvae. Fishery Bull. Fish Wildl. Serv. U.S. 58, 293–304 (1958)Google Scholar
  4. Gibor, A.: Some ecological relationships between phyto- and zooplankton. Biol. Bull. mar. biol. Lab., Woods Hole 111, 230–234 (1956)CrossRefGoogle Scholar
  5. Hobbie, J.E., R.J. Daley and S. Jasper: Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl. envirl Microbiol. 33, 1225–1228 (1977)Google Scholar
  6. Loosanoff, V.L. and H.C. Davis: Rearing of bivalve mollusks. In: Advances in marine biology, Vol. 1. pp 1–136. Ed. by F.S. Russell. London and New York: Academic Press 1963Google Scholar
  7. Mackas, D. and R. Bohrer: Fluorescence analysis of zooplankton gut contents and an investigation of diel feeding patterns. J. exp. mar. Biol. Ecol. 25, 77–85 (1976)CrossRefGoogle Scholar
  8. Provasoli, L., K. Shiraishi and J.R. Lance: Nutritional idiosyncrasies of Artemia and Tigriopus in monoxenic culture. Ann. N.Y. Acad. Sci. 77, 250–261 (1959)CrossRefGoogle Scholar
  9. Ukeles, R.: Nutritional requirements in shellfish culture. In: Proceedings of the conference on artificial propagation of commercially valuable shellfish — oysters, pp 43–64. Ed. by K.S. Price and D.L. Maurer. Newark, Delaware: University of Delaware 1971Google Scholar
  10. —: Continuous culture — a method for the production of unicellular algal foods. In: Handbook of phycological methods, pp 233–254. Ed. by J. Stein. London: Cambridge University Press 1973Google Scholar
  11. — and B.M. Sweeney: Influence of dinoflagellate trichocysts and other factors on the feeding of Crassostrea virginica larvae on Monochrysis lutheri. Limnol. Oceanogr. 14, 403–410 (1969)CrossRefGoogle Scholar
  12. Walne, P.R.: Observations on the food value of seven species of algae to the larvae of Ostrea edulis L. I. Feeding experiments. J. mar. biol. Ass. U.K. 43, 767–784 (1963)CrossRefGoogle Scholar
  13. —: The culture of marine bivalve larvae. In: Physiology of Mollusca, 1, pp 197–210. Ed. by K. Wilbur and C.M. Yonge. New York and London: Academic Press 1964CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • J. Babinchak
    • 1
  • R. Ukeles
    • 1
  1. 1.Northeast Fisheries Center, Milford LaboratoryNational Marine Fisheries ServiceMilfordUSA

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