Comparison of osmoregulation between Adriatic and North Sea Carcinus

Abstract

In two spatially isolated species — North Sea Carcinus maenas L. and Adriatic Carcinus mediterraneus Czerniavsky —osmoconcentration in hemolymph and Na-transport were investigated. The experiments were performed on crabs which had been long-term acclimated to sea water (1190 mOsmol/l) and to 50% sea water, and on individuals immediately after transfer from sea water to 50% sea water, until new steadystate conditions had been reached. About 10 to 12 h after transfer, a new steady state of total osmoconcentration as well as of the concentrations of the most representative cations Na, K, Ca, and Mg was attained. The hemolymph contents of Na and total osmotically active substances of the North and Adriatic Sea populations are significantly different when they are long-term acclimated to sea water and to 50% sea water. Total sodium fluxes, as calculated from influx and outflux experiments, are in the range 12.1 to 15.2 μM Na/g/h in both species when acclimated to sea water. In 50% sea water, sodium fluxes are retarded, especially in C. maenas (6.6 μM Na/g/h). This value is significantly lower than the corresponding value in C. mediterraneus (11.4 μM Na/g/h). Differences are discussed on the basis of adaptation of the populations to their different environments.