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Profiles of photosynthetic pigments in the ocean using thin-layer chromatography

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Abstract

Phytoplankton pigments at a coastal station off Sydney, Australia, were studied by cellulose thinlayer chromatography. The chromatographic procedure distinguished chlorophylls a, b and c, chlorophyllide a, pheophytin a and pheophorbide a, and the major carotenoids carotene, astaxanthin, fucoxanthin, peridinin, diadinoxanthin and neoxanthin. Chlorophyllide a and pheophorbide a were the most significant chlorophyll-a degradation products in the water column, chlorophyllide a coming from chlorophyllase activity of senescent diatoms, and pheophorbide a from faecal pellets of copepods. Chlorophyll c occurred in every sample, even where there was no trace of chlorophyll a. Because of the greater chemical and biological stability of chlorophyll c compared to chlorophyll a, high chlorophyll c:a ratios result from a large proportion of senescent or detrital material in the samples. Determining the position of patches of copepods, diatoms, green algae and dinoflagellates in the water column was easily done by noting the presence of definitive pigments on the chromatograms.

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Literature Cited

  • Barrett, J. and S.W. Jeffrey: Chlorophyllase and formation of an atypical chlorophyllide in marine algae. Pl. Physiol., Lancaster 39, 44–47 (1964)

    Google Scholar 

  • —: A note on the occurrence of chlorophyllase in marine algae. J. exp. mar. Biol. Ecol. 7, 255–262 (1971)

    Google Scholar 

  • Currie, R.I.: Pigments in zooplankton faeces. Nature, Lond. 193, 956–957 (1962)

    Google Scholar 

  • Davis, P.S.: A method for the determination of chlorophyll in seawater. Tech. Rep. Div. Fish. Oceanogr. C.S.I.R.O. Aust. 7, 1–8 (1957)

    Google Scholar 

  • Holm-Hansen, O., C.J. Lorenzen, R.W. Holmes and J.D.H. Strickland: Fluorimetric determination of chlorophyll. J. Cons. perm. int. Explor. Mer 30, 3–15 (1965)

    Google Scholar 

  • Humphrey, G.F.: Phytoplankton pigments in the Pacific Ocean. Proceedings of the Conference on Primary Productivity Measurements, Marine and Freshwater, University of Hawaii, 1961 (Ed. M. S. Doty). (Monogr. U.S. atom. Energy Commn).

  • Jeffrey, S.W.: Paper chromatographic separation of pigments in marine phytoplankton. Aust. J. mar. Freshwat. Res. 16, 307–313 (1965)

    Google Scholar 

  • —: Quantitative thin-layer chromatography of chlorophylls and carotenoids from marine algae. Biochim. biophys. Acta 162, 271–285 (1968)

    Google Scholar 

  • —: Properties of two spectrally different components in chlorophyll c preparations. Biochim. biophys. Acta 177, 456–467 (1969)

    Google Scholar 

  • —: Preparation and some properties of cyrstalline chlorophyll c 1 and c 2 from marine algae. Biochim. biophys. Acta 279, 15–33 (1972)

    Google Scholar 

  • — and M.B. Allen: Pigments, growth and photosynthesis in cultures of two chrysomonads, Coccolithus huxleyi and a Hymenomonas sp. J. gen. Microbiol. 36, 277–288 (1964)

    Google Scholar 

  • —, R. Douce and A.A. Benson: Carotenoid transformations in the chloroplast envelope. Proc. natn Acad. Sci. U.S.A. 71, 807–810 (1974)

    Google Scholar 

  • Jitts, H.R.: The simulated in situ measurement of oceanic primary production. Aust. J. mar. Freshwat. Res. 14, 139–147 (1963)

    Google Scholar 

  • Lees, T.M. and P.J. de Muria: A simple method for the preparation of thin layer chromatography plates. J. Chromat. 8, 108–109 (1962)

    Google Scholar 

  • Loftus, M.E. and J.H. Carpenter: A fluorimetric method for determining chlorophylls a, b and c. J. mar. Res. 29, 319–338 (1971)

    Google Scholar 

  • Lorenzen, C.J.: A note on the chlorophyll and pheophytin content of the chlorophyll maximum. Limnol. Oceanogr. 10, 482–483 (1965)

    Google Scholar 

  • —: A method for the continuous measurement of in vivo chlorophyll concentration. Deep Sea Res. 13, 223–227 (1966)

    Google Scholar 

  • —: Determination of chlorophyll and pheopigments: spectrophotometric equations. Limnol. Oceanogr. 12, 343–346 (1967a)

    Google Scholar 

  • —: Vertical distribution of chlorophyll and pheopigments: Baja California. Deep Sea Res. 14, 735–745 (1967b)

    Google Scholar 

  • Patterson, J. and T.R. Parsons: Distribution of chlorophyll a and degradation products in various marine materials. Limnol. Oceanogr. 8, 355–356 (1963)

    Google Scholar 

  • Saijo, Y. and T. Kamiya: Occurrence of chlorophyllide a in the sea. In: Biological oceanography of the Northern Pacific Ocean, pp 191–197. Ed. by A.Y. Takenouti. Tokyo: Idemitsu 1973

    Google Scholar 

  • SCOR-UNESCO: Monographs on oceanographic methodology, Vol. 1. 17 pp. Paris: U.N.E.S.C.O. 1966

    Google Scholar 

  • Stransky, H. and A. Hager: The carotenoid pattern and the occurrence of the light-induced xanthophyll cycle in various classes of algae. VI. Chemosystematic study. Arch. Mikrobiol. 73, 315–323 (1970)

    Google Scholar 

  • Strickland, J.D.H.: Research on the marine planktonic food web at the Institute of Marine Resources: a review of the past 7 years of work. Oceanogr. mar. Biol. A. Rev. 10, 349–414 (1972)

    Google Scholar 

  • Yentsch, C.S.: Distribution of chlorophyll and pheophytin in the open sea. Deep Sea Res. 12, 653–666 (1965)

    Google Scholar 

  • — and D.W. Menzel: A method for the determination of phytoplankton chlorophyll and pheophytin by fluorescence. Deep Sea Res. 10, 221–231 (1963)

    Google Scholar 

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Authors and Affiliations

  1. CSIRO Marine Biochemistry Unit, Sydney University, Sydney, New South Wales, Australia

    S. W. Jeffrey

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  1. S. W. Jeffrey
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Communicated by T.R. Parsons, Vancouver

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Jeffrey, S.W. Profiles of photosynthetic pigments in the ocean using thin-layer chromatography. Marine Biology 26, 101–110 (1974). https://doi.org/10.1007/BF00388879

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