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ATP concentration and adenylate energy charge of tropical zooplankton from waters inside the great barrier reef

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Abstract

The adenylate energy charge ratio (EC) of 8 species of zooplankton ranged from 0.70 to 0.89. Eucalanus subcrassus had significantly (P<0.05) lower EC when captured during a 30-min tow than during a 3-min tow, indicating stress at capture. The concentration of ATP in the 8 species ranged from 9.1 to 36.0 μg/mg protein and from 0.17 to 15.7 μg/mg dry weight. Excluding a low value for the ctenophore Pleurobrachia sp., the average concentration was 8.1 μg ATP/mg dry weight, which is equivalent to an approximate C/ATP ratio of 50.

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Literature Cited

  • Ansell, A. D.: The adenosine triphosphate content of some marine bivalve molluscs. J. exp. mar. Biol. Ecol. 28, 269–283 (1977)

    Article  Google Scholar 

  • Ausmus, B. S.: The use of the ATP assay in terrestrial decomposition studies. Bull. ecol. Res. Comm. Stockholm 17, 223–234 (1973)

    Google Scholar 

  • Balch, N.: ATP content of Calanus finmarchicus. Limnol. Oceanogr. 17, 906–908 (1972)

    Google Scholar 

  • Berland, B. R., D. J. Bonin, P. L. Laborde et S. Y. Maestrini: Variations de quelques facteurs estimatifs de la biomasse, et en particulier de l'ATP chez plusieurs algues marines planctoniques. Mar. Biol. 13, 338–345 (1972)

    Google Scholar 

  • Biggs, D. C.: Respiration and ammonium excretion by open ocean gelatinous zooplankton. Limnol. Oceanogr. 22, 108–117 (1977)

    Google Scholar 

  • Blair, J. McD.: Magnesium, potassium, and the adenylate kinase equilibrium. Magnesium as a feedback signal from the adenine nucleotide pool. Eur. J. Biochem. 13, 384–390 (1970)

    PubMed  Google Scholar 

  • Cavari, B.: ATP in Lake Kinneret: Indicator of microbial biomass or of phosphorus deficiency? Limnol. Oceanogr. 21, 231–236 (1976)

    Google Scholar 

  • Chapman, A. G. and D. E. Atkinson: Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Adv. microb. Physiol. 15, 253–306 (1977)

    PubMed  Google Scholar 

  • Falkowski, P. G.: The adenylate energy charge in marine phytoplanton: The effect of temperature on the physiological state of Skeletonema costatum (Grev.) Cleve. J. exp. mar. Biol. Ecol. 27, 37–45 (1977)

    Article  Google Scholar 

  • Hamner, W. M. and J. H. Carleton: Copepod swarms: Attributes and role in coral reef ecosystems. Limnol. Oceanogr. 24, 1–14 (1979)

    Google Scholar 

  • Holm-Hansen, O.: ATP levels in algal cells as influenced by environmental conditions. Pl. Cell Physiol., Tokyo 11, 689–700 (1970)

    Google Scholar 

  • Holm-Hansen, O.: The use of ATP determinations in ecological studies. Bull. ecol. Res. Comm. Stockholm 17, 215–222 (1973)

    Google Scholar 

  • Holm-Hansen, O. and C. R. Booth: The measurement of adenosine triphosphate in the ocean and its ecological significance. Limnol. Oceanogr. 11, 510–519 (1966)

    Google Scholar 

  • Ikeda, T.: Nutritional ecology of marine zooplankton. Mem. Fac. Fish. Hokkaido Univ. 22, 1–97 (1974)

    Google Scholar 

  • Karl, D. M.: Distribution, abundance, and metabolic states of microorganisms in the water column and sediments of the Black Sea. Limnol. Oceanogr. 23, 936–949 (1978)

    Google Scholar 

  • Karl, D. M., J. A. Haugsness, L. Campbell and O. Holm-Hansen: Adenine nucleotide extraction from multicellular organisms and beach sand: ATP recovery, energy charge ratios and determination of carbon/ATP ratios. J. exp. mar. Biol. Ecol. 34, 163–181 (1978)

    Article  Google Scholar 

  • Karl, D. M. and O. Holm-Hansen: Methodology and measurement of adenylate energy charge ratios in environmental samples. Mar. Biol. 48, 185–197 (1978)

    Google Scholar 

  • Kimmich, G. A., J. Randles and J. S. Brand: Assay of picomole amounts of ATP, ADP, and AMP using the luciferase enzyme system. Analyt. Biochem. 69, 187–206 (1975)

    PubMed  Google Scholar 

  • Knowles, C. J.: Microbial metabolic regulation by adenine nucleotide pools. In: Microbial energetics, pp 241–283. Ed. by B. A. Haddock and W. A. Hamilton. Cambridge: Cambridge Univ. Press 1977

    Google Scholar 

  • Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    PubMed  Google Scholar 

  • Lundin, A. and A. Thore: Comparison of methods for extraction of bacterial adenine nucleotides determined by firefly assay. Appl. Microbiol. 30, 713–721 (1975)

    PubMed  Google Scholar 

  • Markland, F. S. and C. L. Wadkins: Adenosine triphosphateadenosine 5′-monophosphate phosphotransferase of bovine liver mitochondria. II. General kinetic and structural properties. J. biol. Chem. 241, 4136–4145 (1966)

    PubMed  Google Scholar 

  • Niven, D. F., P. A. Collins and C. J. Knowles: Adenylate energy charge during batch culture of Beneckea natriegens. J. gen. Microbiol. 98, 95–108 (1977)

    PubMed  Google Scholar 

  • Noda, L.: Adenylate kinase. In: The enzymes, vol. 8, pp 279–305. Ed. by P. D. Boyer. New York: Academic Press 1973

    Google Scholar 

  • Pamatmat, M. M. and H. R. Skjoldal: Metabolic activity, adenosine phosphates and energy charge of belowground biomass of Juncus roemerianus Scheele and Spartina alterniflora Loisel. Estuar. cstl mar. Sci. 9, 79–90 (1979)

    Google Scholar 

  • Reeve, M. R. and L. D. Baker: Production of two planktonic carnivores (chaetognath and ctenophore) in South Florida inshore waters. Fish. Bull. U.S. 73, 238–248 (1975)

    Google Scholar 

  • Roberton, A. M. and R. S. Wolfe: Adenosine triphosphate pools in Methanobacterium. J. Bact. 102, 43–51 (1970)

    PubMed  Google Scholar 

  • Sakshaug, E. and O. Holm-Hansen: Chemical composition of Skeletonema costatum (Grev.) Cleve and Pavlova (Monochrysis) lutheri (Droop) Green as a function of nitrate-, phosphate-, and iron-limited growth. J. exp. mar. Biol. Ecol. 29, 1–34 (1977)

    Article  Google Scholar 

  • Skjoldal, H. R. and U. Bamstedt: Ecobiochemical studies on the deep-water pelagic community of Korsfjorden, western Norway. Adenine nucleotides in zooplankton. Mar. Biol. 42, 197–211 (1977)

    Google Scholar 

  • Sokal, R. R. and F. J. Rohlf: Biometry, 776 pp. San Francisco: W. H. Freeman 1969

    Google Scholar 

  • Swedes, J. S., R. J. Sedo and D. E. Atkinson: Relation of growth and protein synthesis to the adenylate energy charge in an adenine-requiring mutant of Escherichia coli. J. biol. Chem. 250, 6930–6938 (1975)

    PubMed  Google Scholar 

  • Traganza, E. D. and K. J. Graham: Carbon/adenosine triphosphate ratios in marine zooplankton. Deep-Sea Res. 24, 1187–1193 (1977)

    Google Scholar 

  • Wiebe, W. J. and K. Bancroft: Use of the adenylate energy charge ratio to measure growth state of natural microbial communities. Proc. natn. Acad. Sci. U.S.A. 72, 2112–2115 (1975)

    Google Scholar 

  • Witzel, K.-P.: The adenylate energy charge as a measure of microbial activities in aquatic habitats. Arch. Hydrobiol., Beih., Ergeb. Limnol. 12, 146–165 (1979)

    Google Scholar 

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Authors and Affiliations

  1. Institute of Marine Biology, University of Bergen, N-5065, Blomsterdalen, Norway

    H. R. Skjoldal

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  1. H. R. Skjoldal
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Communicated by T. Fenchel, Aarhus

Contribution from the Australian Institute of Marine Science, Australia, and the Institute of Marine Biology, University of Bergen, Norway

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Skjoldal, H.R. ATP concentration and adenylate energy charge of tropical zooplankton from waters inside the great barrier reef. Mar. Biol. 62, 119–123 (1981). https://doi.org/10.1007/BF00388173

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