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Studies on the carbohydrases in the digestive tract of the milkfish chanos chanos

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Abstract

Crude extracts from various regions of the digestive tract of pond grown milkfish were tested forttheir ability to catalyze the hydrolysis of various carbohydrates. The most active carbohydrases were those involved in the hydrolysis of α-glucosidic bonds. Maltose, trehalose, dextrin, starch and glycogen were rapidly hydrolyzed in the presence of crude extracts from the intestines and the pyloric caeca. High amylase activity was observed in extracts from the intestines, pancreas, pyloric caeca and liver. The intestinal amylase had optimum activity at pH 6.2 and at a temperature of about 50°C. It was active at a chloride concentration of 10 to 40 ppt. The amylase activity in the intestines consistently peaked daily at about noon when the milkfish gut was full. In contrast, enzyme activity was significantly lower at 0030 hrs when the gut was empty. These results are consistent with earlier observations that the milkfish is a daytime feeder and suggest further that intestinal amylase secretion is in phase with the feeding activity of the milkfish. Although the fishes used in this study fed mostly on the naturally occurring algae in the ponds, no cellulase activity was detected in any region of the digestive tract. Less active carbohydrases that were detected include a β-glucosidase and β-galactosidase, both of which were of limited substrate specificity.

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Authors and Affiliations

  1. Brackishwater Aquaculture Center, University of the Philippines, Iloilo City, Philippines

    Y. N. Chiu

  2. Aquaculture Department, Southeast Asian Fisheries Development Center, P. O. Box 256, Iloilo City, Philippines

    L. V. Benitez

Authors
  1. Y. N. Chiu
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  2. L. V. Benitez
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Communicated by O. Kinne, Hamburg

SEAFDEC Contribution No. 71

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Chiu, Y.N., Benitez, L.V. Studies on the carbohydrases in the digestive tract of the milkfish chanos chanos . Mar. Biol. 61, 247–254 (1981). https://doi.org/10.1007/BF00386667

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