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Marine Biology

, Volume 120, Issue 1, pp 47–53 | Cite as

The systemic heart of Octopus vulgaris: effects of exogenous arachidonic acid and capability for arachidonate metabolism

  • C. Agnisola
  • R. Venzi
  • T. Mustafa
  • B. Tota
Article
  • 64 Downloads

Abstract

The actions of exogenous arachidonic acid on the performance of the isolated and perfused systemic heart of Octopus vulgaris (collected in the Bay of Naples in 1992), and the potential of this heart for eicosanoid synthesis are described. Arachidonic acid induces positive chronotropic and inotropic effects. The positive inotropic effect is apparent only at the lowest concentration used (10−7M) and in the paced heart preparations, in which a negative relationship between stroke volume and heart rate has been demonstrated. Using 10−5M arachidonate, which induces the greatest chronotropic effect, a reduction of inotropism is evident which is due to the above negative relationship between stroke volume and heart rate. These effects are scarcely affected by the cyclooxygenase and lipoxygenase inhibitors, indomethacin (IM) and nordihydroguaiaretic acid (NDGA), respectively. On the coronary system of this heart arachidonic acid displays a potent vasoconstrictory action (ca. 100 percent increase of coronary resistance). This effect is potentiated by IM and reduced by NDGA. 14C-arachidonate is metabolized by the octopus ventricle homogenate into the lipoxygenase products (assayed as hydroxy acids) more actively than into cyclooxygenase products (prostanoids: PGE2, PGD2, PGF and 6-keto-PGF). On the other hand, the Ca-ionophore A23187 enhances the production of cyclooxygenase metabolites much more than of lipoxygenase metabolites.

Keywords

Arachidonic Acid PGE2 Stroke Volume Arachidonate Positive Inotropic Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • C. Agnisola
    • 1
    • 2
  • R. Venzi
    • 2
  • T. Mustafa
    • 3
  • B. Tota
    • 2
    • 4
  1. 1.Dipartimento di Fisiologia Generale ed AmbientaleUniversità degli Studi di Napoli “Federico II”NapoliItaly
  2. 2.Stazione Zoologica “A. Dohrn”NapoliItaly
  3. 3.Biologisk InstitutOdense UniversitetOdenseDenmark
  4. 4.Dipartimento di Biologia CellulareUniversità della CalabriaArcavacata di Rende (CS)Italy

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