Marine Biology

, Volume 120, Issue 1, pp 41–45 | Cite as

Proliferation kinetics of mussel (Mytilus galloprovincialis) gill cells

  • M. J. Martínez-Expósito
  • J. J. Pasantes
  • J. Méndez


A technique of sister chromatid differentiation (SCD) using bromodeoxyuridine (BrdU) incorporation and a modification of the fluorescence plus Giemsa (FPG) method was employed to determine cell-proliferation kinetics in gill tissue of the mussel Mytilus galloprovincialis. Dose-dependent proliferation inhibition was examined. In vivo administration of BrdU for 12, 24, 36, 48, 60, 72, 84, and 96 h was studied. Our data show that the highest yield of second-generation metaphase plates is obtained after 60 h BrdU treatment; the duration of a cell cycle is 24 to 30 h. On the basis of these data, a BrdU incorporation period of 48 to 60 h would seem to be most appropriate for the sister chromatid exchange (SCE) tests carried out in gill cells of M. galloprovincialis, whereas the 12 to 24 h exposure would give the best results for replication band analysis.


Cell Cycle Sister Chromatid BrdU Incorporation Sister Chromatid Exchange Bromodeoxyuridine 
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. J. Martínez-Expósito
    • 1
  • J. J. Pasantes
    • 1
  • J. Méndez
    • 1
  1. 1.Departamento Biología Celular y Molecular, Área de GenéticaUniversidad de La CoruãLa CoruñaSpain

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