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Cytotechnology

, Volume 6, Issue 2, pp 131–136 | Cite as

Erythropoietin gene expression in haemopoietic cell lines

  • Enam Abood
  • Sandro Eridani
  • Barbara Sawyer
  • Nigel Westwood
  • Thomas C. Pearson
Original Research Paper
  • 32 Downloads

Abstract

Erythropoietin (Epo) gene expression was studied in a number of different haemopoietic cell lines by in situ hybridization and Northern Blot analysis using a radioisotope-labelled monkey Epo DNA probe. A positive message was expressed by a human cell line, CM-S, derived from a patient with congenital hypoplastic anemia, and by a murine erythro-leukaemic cell line, clone 707, derived from the spleen of Friend virus-infected mice. No message was detected in two megakaryoblastic cell lines, and in a monocytic cell line, derived from a patient with acute monocytic leukaemia. These data may fit with the hypothesis of expression of Epo and other growth factors by haemopoietic cells through a mechanism of so-called autocrine secretion.

Key words

erythropoietin monocyte-macrophage system in situ hybridization Northern blotting autocrone stimulation 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Enam Abood
    • 1
  • Sandro Eridani
    • 1
  • Barbara Sawyer
    • 1
  • Nigel Westwood
    • 1
  • Thomas C. Pearson
    • 1
  1. 1.Division of HaematologyUMDSLondonUK

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