Abstract
Erythropoietin (Epo) gene expression was studied in a number of different haemopoietic cell lines by in situ hybridization and Northern Blot analysis using a radioisotope-labelled monkey Epo DNA probe. A positive message was expressed by a human cell line, CM-S, derived from a patient with congenital hypoplastic anemia, and by a murine erythro-leukaemic cell line, clone 707, derived from the spleen of Friend virus-infected mice. No message was detected in two megakaryoblastic cell lines, and in a monocytic cell line, derived from a patient with acute monocytic leukaemia. These data may fit with the hypothesis of expression of Epo and other growth factors by haemopoietic cells through a mechanism of so-called autocrine secretion.
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Abood, E., Eridani, S., Sawyer, B. et al. Erythropoietin gene expression in haemopoietic cell lines. Cytotechnology 6, 131–136 (1991). https://doi.org/10.1007/BF00373030
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DOI: https://doi.org/10.1007/BF00373030