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The contribution of bovine leukaemia virus infected B-cells to the number of circulating B-cells in cattle

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Abstract

This study used a combination of single antigen immunoperoxidase staining for bovine leukaemia virus (BLV) p24 capsid antigen and IgM to enumerate the peripheral blood mononuclear cells (PBMCs) infected with BLV and the number of B-cells, respectively. A significant relationship was found between the number of BLV-infected PBMCs and the number of circulating B-cells. A model was created that predicted the number of circulating B-cells using the number of BLV-infected PBMCs. These data also show that the percentage of B-cells in PBMCs preparations is not affected by 24 h of in vitro culture when LPS is added to the culture medium. Double antigen labelling showed that the majority of circulating B-cells were infected with BLV in some BLV-seropositive cattle that were not persistently lymphocytotic.

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References

  • Depelchin A, Lettesson JJ, Lostrie-Trussart N et al. (1989) Bovine leukemia virus (BLV)-infected B-cells express a marker similar to the CD5 T cell marker. Immunol Lett 20:69–76

    Google Scholar 

  • Diglio CA, Ferrer JF (1976) Induction of syncytia by the bovine Ctype leukemia virus. Cancer Res 36:1056–1067

    Google Scholar 

  • Driscoll DM, Olson C (1977) Bovine leukemia virus-associated antigens in lymphocyte cultures. Am J Vet Res 38:1897–1898

    Google Scholar 

  • Esteban EN, Thorn RM, Ferrer JF (1985a) Characterization of the blood lymphocyte population in cattle infected with the bovine leukemia virus. Cancer Res 45:3225–3230

    Google Scholar 

  • Esteban EN, Thorn RM, Ferrer JF (1985b) An amplified immunoperoxidase assay to detect bovine leukemia virus expression: development and comparison with other assays. Cancer Res 45:3231–3235

    Google Scholar 

  • Ferrer JF (1980) Bovine lymphosarcoma. Adv Vet Sci Comp Med 24:1–68

    Google Scholar 

  • Ferrer JF, Diglo CA (1976) Development of an in vitro infectivity assay for the C-type bovine leukemia virus. Cancer Res 36:1068–1073

    Google Scholar 

  • Fossum C, Burny A, Portetelle D et al. (1988) Detection of B and T cells, with lectins or antibodies, in healthy and bovine leukemia virus-infected cattle. Vet Immunol Immunopathol 18:269–278

    Google Scholar 

  • Galfré G, Howe SC, Milstein C et al. (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266:550–552

    Google Scholar 

  • Heeney JL, Valli PJS, Jacobs RM et al (1992) Evidence for Bovine Leukemia Virus infection of peripheral blood monocytes and limited antigen expression in bovine lymphoid tissue. Lab Invest 66:608–617

    Google Scholar 

  • Henry C, Marbrook J, Vann DC et al. (1980) Limiting dilution analysis. In: Mishell BB, Shiigi SM (eds) Selected methods in cellular immunology, 1st edn, W. H. Freeman, San Francisco, pp 138–152

    Google Scholar 

  • Jensen WA, Sheehy SE, Fox MH et al. (1990) In vitro expression of bovine leukemia virus in isolated B-lymphocytes of cattle and sheep. Vet Immunol Immunopathol 26:333–342

    Google Scholar 

  • Kaja RW, Olson C, Rowe RF, et al. (1984) Establishment of a bovine leukosis virus-free dairy herd. J Am Vet Med Assoc 184:184–185

    Google Scholar 

  • Kenyon SJ, Piper CE (1977a) Properties of density gradient-fractionated peripheral blood leucocytes from cattle with bovine leukemia virus. Infect Immun 16:891–897

    Google Scholar 

  • Kenyon SJ, Piper CE (1977b) Cellular basis of persistent lymphocytosis in cattle infected with bovine leukemia virus. Infect Immun 16:889–893

    Google Scholar 

  • Kettmann R, Cleuter Y, Mammerickx M et al. (1980a) Genomic integration of bovine leukemia provirus: comparison of persistent lymphocytosis with lymph node tumor form of enzootic bovine leukosis. Proc Natl Acad Sci USA 77:2577–2581

    Google Scholar 

  • Kettmann R, Marbaix G, Cleuter Y et al. (1980b) Genomic integration of bovine leukemia provirus and lack of viral RNA expression in the target cells of cattle with different responses to BLV infection. Leukemia Res 4:509–519

    Google Scholar 

  • Lagarias DM, Radke K (1989) Transcriptional activation of BLV in blood cells from experimentally infected asymptomatic sheep with latent infections. J Virol 63:2099–2107

    Google Scholar 

  • Lettesson JJ, Van den Broecke A, Marbaix-Cleuter Y et al. (1991) FACS analysis of bovine leukemia virus (BLV)-infected cell lines with monoclonal antibodies (mAbs) to B cells and to monocytes/ macrophages. Vet Immunol Immunopathol 27:207–213

    Google Scholar 

  • Lewin HA, Wu MC, Nolan TJ et al. (1988) Peripheral B lymphocyte percentage as an indicator of subclinical progression of bovine leukemia virus infection. J Dairy Sci 71:2526–2534.

    Google Scholar 

  • Matheise JP, Delcommenne M, Mager A et al. (1992) CD5 + B cells from bovine leukemia virus infected cows are activated cycling cells responsive to interleukin 2. Leukemia 6:304–309

    Google Scholar 

  • Miller JM, Miller LD, Olson C et al. (1969) Virus-like particles in phytohemagglutinin-stimulated lymphocyte cultures with reference to bovine lymphosarcoma. J Natl Cancer Inst 43:1297–1305

    Google Scholar 

  • Moulton JE, Harvey JW (1990) Tumors of lymphoid and haematopoietic tissues. In: JE Moulton (ed) Tumors in domestic animals, 3rd edn, University of California Press, Berkeley, pp 249–255

    Google Scholar 

  • Muscoplat CC, Johnson DW, Pomeroy KA, Olson JM, Larson V, Stephens JB, Sorenson DK (1974) Lymphocyte surface immunoglobulin: frequency in normal and lymphocytotic cattle. Am J Vet Res 35:593–595

    Google Scholar 

  • Paul PS, Pomeroy KA, Castro AE et al. (1977) Detection of bovine leukemia virus in B-lymphocytes by the syncytia induction assay. J Natl Cancer Inst 59:1269–1272

    Google Scholar 

  • Paul PS, Senogles PR, Muscoplat CC et al. (1979) Enumeration of Tcells, B-cells, and monocytes in the peripheral blood of normal and lymphocytotic cattle. Clin Exp Immunol 35:306–316

    Google Scholar 

  • Perman V, Dirks VA, Fangmann G et al. (1970) Statistical evaluation of lymphocyte values on Minnesota dairy cattle. Am J Vet Res 31:1217–1222

    Google Scholar 

  • Remington RD, Schork MA (1985a) Regression and correlation. In: Statistics and applications to the biological and health sciences, 2nd edn. Prentice-Hall, Englewood Cliffs, NJ, pp 235–261

    Google Scholar 

  • Remington RD, Schork MA (1985b) Distribution-free and nonparametric methods. In: Statistics with applications to the biological and health sciences, 2nd edn. Prentice-Hall, Englewood Cliffs, NJ, pp 310–324

    Google Scholar 

  • Rovnak J, Casey JW, Boyd AL et al. (1991) Isolation of bovine leukemia virus infected endothelial cells from cattle with persistent lymphocytosis. Lab Invest 65:192–202

    Google Scholar 

  • Schultz RD, Adams LS (1978) Immunologic methods for detection of Immoral and cellular immunity. Vet Clin North Am 8:721–753

    Google Scholar 

  • Stott ML, Thurmond MC, Dunn SJ et al. (1991) Integrated bovine leukosis proviral DNA in T helper and T cytotoxic/suppressor lymphocytes. J Gen Virol 72:307–315

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Weiland F, Straus OC (1975) Frequency of surface immunoglobulin bearing blood lymphocytes in cattle affected with bovine leukosis. Res Vet Sci 19:100–105

    Google Scholar 

  • Williams DL, Amborski GF, Davis WC (1988a) Enumeration of T and B lymphocytes in bovine leukemia virus-infected cattle, using monoclonal antibodies. Am J Vet Res 49:1098–1103

    Google Scholar 

  • Williams DL, Barta O, Amborski GF (1988b) Molecular studies of Tlymphocytes from cattle infected with bovine leukemia virus. Vet Immunopathol 19:307–323

    Google Scholar 

  • Van de Maaten MJ, Miller JM (1975) Replication of bovine leukemia virus in monolayer cell cultures. In: Clemmsen J, Yohn DS (eds) Comparative leukemia research. Research Bibl Haematol 43:360–362

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Authors and Affiliations

  1. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, USA

    P. M. Kramme, C. B. Thomas & D. Schultz

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  1. P. M. Kramme
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  2. C. B. Thomas
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  3. D. Schultz
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Kramme, P.M., Thomas, C.B. & Schultz, D. The contribution of bovine leukaemia virus infected B-cells to the number of circulating B-cells in cattle. Comp Haematol Int 4, 96–101 (1994). https://doi.org/10.1007/BF00368275

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