Abstract
The high speed supernatant fraction of a homogenate of Squalus acanthias L. liver catalyses the oxidation of oleyl alcohol to oleic acid by an NAD-dependent process. Reduction of fatty acid to fatty alcohol could not be demonstrated. Liver lipids of S. acanthias contain about 60 and 40% of triglycerides and diacyl glyceryl ethers, respectively, with only traces of wax esters. Serum lipids contain 28, 15 and 26% of triglycerides, diacyl glyceryl ethers and wax esters, respectively. Cell-free fractions of S. acanthias liver catalyse the formation of wax esters by an ATP1-dependent process. The bulk of wax ester synthesis occurs in the high-speed supernatant fraction of liver. The rate of formation of the ester bond in wax esters is comparable to the rate of synthesis of ester bonds in triglycerides, and greatly exceeds the rate of formation of the ether bond in diacyl glyceryl ethers. Results are discussed in terms of possible factors controlling the levels of neutral lipids in S. acanthias.
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Literature cited
Bergstrom, S. and B. Borgstrom: Some aspects of the intestinal absorption of fats. Prog. Chem. Fats 3, 351–393 (1956).
Bligh, E. G. and W. J. Dyer: A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917 (1959).
Bone, Q. and B. L. Roberts: The density of elasmobranchs. J. mar. biol. Ass. U.K. 49, 913–937 (1969).
Corner, E. D. S., E. J. Denton and G. R. Forster: On the buoyancy of some deep-sea sharks. Proc. R. Soc. (B)171, 415–429 (1969).
Friedberg, S. J. and R. C. Greene: The enzymic synthesis of wax in liver. J. biol. Chem. 242, 234–237 (1967).
Gauglitz, E. J. and D. C. Malins: The preparation of polyunsaturated aliphatic aldehydes via the acyloin condensation. J. Am. Oil Chem. Soc. 37, 425–427 (1960).
Hansen, I. A. and C. C. Cheah: Related dietary and tissue lipids of the sperm whale. Comp. Biochem. Physiol. 31, 757–761 (1969).
Hickling, C. F.: A contribution towards the life history of the spur-dog. J. mar. biol. Ass. U.K. 16, 529–577 (1930).
Kolattukudy, P. E.: Mechanisms of synthesis of waxy esters in broccoli (Brassica olerace). Biochemistry, N.Y. 6, 2705–2717 (1967).
Reduction of fatty acids to alcohols by cell-free preparations of Euglena gracilis. Biochemistry, N.Y. 9, 1095–1102 (1970).
Lewis, R. W.: Studies on the stomach oils of marine animals — 1. Oils of the black shark Dalatias licha (Bonnaterre). Comp. Biochem. Physiol. 31, 715–724 (1969).
Densities of three classes of marine lipids in relation to their possible roles as hydrostatic agents. Lipids 5, 151–153 (1970).
Linn, T. C.: The effect of cholesterol feeding and fasting upon β-hydroxy-β-methylglutaryl coenzyme A reductase. J. biol. Chem. 242, 990–993 (1967).
Lowry, O. H., N. J. Rosebrough, R. J. Farr and R. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Malins, D. C.: Lipid metabolism in fish: on the possible role of long-chain alcohols as precursors of plasmalogens. Biochem. J. 101, 1–39 (1966).
A. Barone: Glyceryl ether metabolism: regulation of buoyancy in dogfish (Squalus acanthias). Science, N.Y. 167, 79–80 (1970).
J. R. Sargent: Biosynthesis of alkyl diacyl glycerols and triacyl glycerols in a cell-free system from the liver of dogfish (Squalus acanthias). Biochemistry 10, 1107–1110 (1971).
C. R. Houle: Composition of the diacyl glyceryl ethers and triglycerides of the flesh and liver of the dogfish (Squalus acanthias). J. Lipid Res. 6, 100–105 (1965).
Manwell, C.: A “fetal-maternal shift” in the ovoviviparous spiny dogfish Squalus suckleyi (Girard). Physiol. Zoöl. 31, 93–100 (1958).
Nevenzel, J. C.: Occurrence, function and biosynthesis of wax esters in marine organisms. Lipids 5, 308–319 (1970).
Piiper, J., D. Baumgarten and N. Meyer: Effects of hypoxia upon respiration and circulation in the dogfish Scyliorhinus stellaris. Comp. Biochem. Physiol. 36, 513–520 (1970).
Sand, D. M., J. L. Hehl and H. Schlenk: Biosynthesis of wax esters in fish. Reduction of fatty acids and oxidation of fatty alcohols. Biochemistry, N.Y. 8, 4851–4854 (1969).
Satchell, G. H.: The response of the dogfish to anoxia. J. exp. Biol. 38, 531–543 (1961).
Snyder, F., M. L. Blank, B. Malone and R. L. Wykle: Identification of O-alkyldihydroxyacetone phosphate, O-alkyldihydroxyacetone and diacyl glyceryl ethers after enzymic synthesis. J. biol. Chem. 245, 1800–1805 (1970a).
B. Malone: Enzymic interconversion of fatty alcohols to fatty acids. Biochem. biophys. Res. Commun. 41, 1382–1387 (1970).
M. L. Blank: Enzymic synthesis of O-alkyl bonds in glycerolipids. J. biol. Chem. 245, 1790–1799 (1970b).
Snyder, F., B. Malone, and J. Snoodsma: Metabolism of long chain fatty alcohols: formation of waxes and glyceryl ethers. J. Am. Oil Chem. Soc. 46, 60th Annual Meeting, Abstract No. 9, April (1969).
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Communicated by J. H. S. Blaxter, Oban
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Sargent, J.R., Gatten, R.R. & McIntosh, R. Metabolic relationships between fatty alcohol and fatty acid in the liver of Squalus acanthias . Marine Biology 10, 346–355 (1971). https://doi.org/10.1007/BF00368095
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DOI: https://doi.org/10.1007/BF00368095