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Bioassay of biotin and its distribution in the sea

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Abstract

A simple, rapid and sensitive method of microbiological assay for the determination of biotin in seawater has been developed by using a marine bacterium, Achromobacter sp. (Strain yH-51) as an assay organism. When the organism was incubated at 25°C for 48 h in a synthetic medium with constant shaking, a standard reference curve of bioassay was obtained. The organism is sensitive in the range 0.1 to 8.0 ng/l biotin. The distribution of biotin has been determined in the East China Sea, the North Pacific Ocean, and the bays and inlets along the Pacific coast of Japan. Biotin concentrations in surface waters were in the range from less than 0.1 ng/l up to 57.9 ng/l; generally high in coastal waters and low in deep open waters. The concentrations were generally high in surface waters, and rapidly decreased with depth down to 50 m, below which values were generally low. The vertical distribution of biotin followed closely the concentration of chlorophyll a, with high concentrations of biotin restricted to the euphotic layer of high chlorophyll a contents at most stations observed. Biotin concentrations in surface waters were generally high during summer, but in autumn and winter the concentrations decreased markedly.

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Literature cited

  • Antia, N. J.: A microbiological assay for biotin in sea water. Can. J. Microbiol. 13, 403–409 (1963).

    Google Scholar 

  • Belser, W. L.: Biossay of organic materials in seawater. Int. oceanogr. Congr. 1, 908–910 (1959a).

    Google Scholar 

  • —: Biossay of organic micronutrients in the sea. Proc. natn. Acad. Sci. U.S.A. 45, 1533–1542 (1959b).

    Google Scholar 

  • —: Bioassay of trace substances. In. The sea. Vol. 2. pp 220–231. Ed. by M. N. Hill. New York: Interscience Publisher Inc. 1963.

    Google Scholar 

  • Burkholder, P. R.: Some nutritional relationships among microbes of sea sediments and waters. In: Symposium on marine microbiology, pp 133–150. Ed. by C. H. Oppenheimer. Springfield, Ill.: C. C. Thomas 1963.

    Google Scholar 

  • Carlucci, A. F.: Vitamin B12, thiamine, and biotin. In: The ecology of the plankton off La Jolla, California, in the period April through September, 1967, pp 23–31. Ed. by J. D. H. Strickland. California: University of California Press 1970.

    Google Scholar 

  • — and P. M. Bowes: Production of vitamin B12, thiamine, and biotin by phytoplankton. J. Phycol. 6, 351–357 (1970a)

    Google Scholar 

  • —: Vitamin production and utilization by phytoplankton in mixed culture. J. Phycol. 6, 393–400 (1970b).

    Google Scholar 

  • — and S. B. Silbernagel: Effect of different seawaters on the development of biochemically deficient mutants of Serratia marinorubra. Appl. Microbiol. 13, 663–668 (1965).

    Google Scholar 

  • —: Bioassay of seawater. I. A 14C uptake method for the determination of concentrations of vitamin B12 in seawater. Can. J. Microbiol. 12, 175–183 (1966a).

    Google Scholar 

  • —: Biossay of seawater. II. Methods for the determination of concentrations of dissolved vitamin B1 in seawater. Can. J. Microbiol. 12, 1079–1089 (1966b).

    Google Scholar 

  • —: Bioassay of seawater. IV. The determination of dissolved biotin in seawater using 14C uptake by cells of Amphidinium carteri. Can. J. Microbiol. 13, 979–986 (1967).

    Google Scholar 

  • Droop, M. R.: Organic micronutrients. In: Biochemistry and physiology of algae, pp 131–159. Ed. by R. A. Lewin. New York: Academic Press 1962.

    Google Scholar 

  • Hutner, S. H. and C. A. Bjerknes: Volatile preservatives for culture media. Proc. Soc. exp. Biol. Med. 67, 393–397 (1948).

    Google Scholar 

  • Jeffrey, L. M.: Lipids of marine waters. In: Symposium on organic matter in natural waters. Occ. Publis. Univ. Alaska 1, 55–76 (1970).

  • Jones, G. E.: Suppression of bacterial growth by sea water. In: Symposium on marine microbiology, pp 133–150. Ed. by C. H. Oppenheimer, Springfield, Ill.: C. C. Thomas 1963.

    Google Scholar 

  • Litchfield, C. D. and D. W. Hood: Microbiological assay for organic compounds in seawater. I. Quantitative assay procedures and biotin distribition. Appl. Microbiol. 13, 886–894 (1965).

    Google Scholar 

  • Lyman, J. and R. H. Fleming: Composition of seawater. J. mar. Res. 3, 134–146 (1940).

    Google Scholar 

  • Natarajan, K. V.: Distribution of thiamine, biotin, and niacin in the sea. Appl. Microbiol. 16, 366–369 (1968).

    Google Scholar 

  • Ohwada, K. and N. Taga. Distribution of vitamin B12, thiamine and biotin in marine sediments. J. oceanogr. Soc. Japan 25, 123–136 (1969).

    Google Scholar 

  • Ohwada, K. and N. Taga.: Vitamin B12, thiamine and biotin in Lake Sagami. Limnol. Oceanogr. (In press).

  • Provasoli, L.: Growth factors in unicellular marine algae. In: Perspectives in marine biology, pp 385–403. Ed. by A. A. Buzzati-Traverso. California: University of California Press 1958.

    Google Scholar 

  • —: Organic regulation of phytoplankton fertility. In: The sea. Vol. 2. pp 165–219. Ed. by M. N. Hill. New York: Interscience Publisher Inc. 1963.

    Google Scholar 

  • — and K. Gold: Gyrodinium cohnii, an assay organism for thiamine and biotin. Int. oceanogr. Congr. 1, 931–933 (1959)

    Google Scholar 

  • —: Nutrition of the American strain of Gyrodinium cohnii. Arch. Mikrobiol. 42, 196–203 (1962).

    Google Scholar 

  • Saunders, G. W.: Interrelations of dissolved organic matter and phytoplankton. Bot. Rev. 23, 389–410 (1957).

    Google Scholar 

  • Shewan, J. M., G. Hobbs and W. Hodgkiss: A determinative scheme for the identification of certain genera of gramnegative bacteria, with special reference to the Pseudomonadaceae. J. appl. Bact. 23, 379–390 (1960).

    Google Scholar 

  • Taga, N.: Some ecological aspects of marine bacteria in the Kuroshio Current. In: Proceedings of the U.S. — Japan Seminar on marine microbiology. Bull. Misaki mar. Biol. Inst., Univ. Kyoto 12, 65–76 (1968).

  • Yentsch, C. S. and D. W. Menzel: A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescence. Deep Sea Res. 10, 221–231 (1963).

    Google Scholar 

  • ZoBell, C. E.: Marine microbiology. Chronica bot. 17, 1–240 (1946).

    Google Scholar 

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Communicated by M. Anraku, Nagasaki

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Ohwada, K. Bioassay of biotin and its distribution in the sea. Mar. Biol. 14, 10–17 (1972). https://doi.org/10.1007/BF00365775

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