Abstract
A flow cytometric methodology was set up to assess the binding capability of peripheral blood NK and T cells to the K562 tumor cell line. Differential side scatter characteristics between effectors and targets were used to analyze conjugated and unconjugated cells. The previous labeling of NK and T cells with anti-Leu 11c and anti-Leu 4 monoclonal antibodies, allowed the distinction between unconjugated non-fluorescent and conjugated fluorescent targets and the percentual evaluation of bound anti-Leu 11c+ and anti-Leu 4+ cells.
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Abbreviations
- NK:
-
Natural Killer
- MoAb:
-
Monoclonal Antibody
- SSC:
-
Side Scatter
- FLS:
-
Forward Light Scatter
- PE:
-
Phycoerythrin
- MHC:
-
Major Histocompatibility Complex
- GVHD:
-
Graft Versus Host Disease
References
Bojum AJ (1968) Isolation of leucocytes from human blood further observations — Methylcellulose, dextran, and ficoll as erythrocyte aggregating agents. Scand. J. Clin. Invest. 21(97): 77.
Facchini A, Mariani AR, Papa S, Mariani E and Manzoli FA (1984) Distribution of acid naphtyl acetate esterase among human T lymphocyte subsets. Immunol. lett. 8: 227.
Grimm E and Bonavida B (1979) Mechanism of cell-mediated cytotoxicity at the single cell level. I. Estimation of cytotoxic T lymphocyte frequency and relative lytic efficiency. J. Immunol. 123: 2861.
Herbermann RB and Ortaldo JR (1981) Natural Killer cells. Their role in defense against disease. Science 214: 24.
Herbermann RB, Reynolds CW and Ortaldo JR (1986) Mechanism of cytotoxicity by Natural Killer (NK) cells. Ann. Rev. Immunol. 4: 651.
Papa S, Vitale M, Mariani AR, Roda P, Facchini A and Manzoli FA (1988) Natural killer function in flow cytometry. I. Evaluation of NK lytic activity on K562 cell line. J. Immunol. Meth. 107: 73.
Rubin P, Pross HF and Roder JC (1982) Studies of human natural killer cells. II. Analysis at the single cell level. J. Immunol. 126: 2553.
Storkus WJ, Balber AE and Dawson JR (1986) Quantitation and sorting of vitally stained natural killer cell-target cell conjugates by dual beam flow cytometry. Cytometry 7: 163.
Velardi A, Grossi CE and Cooper MD (1985) A large subpopulation of lymphocytes with T helper phenotype (Leu 3/T4+) exhibits the property of binding to NK cell targets and granular lymphocyte morphology. J. Immunol. 134: 58.
Zarcone D, Tilden AB, Friedman HM and Grossi CE (1987) Human leukemia-derived cell lines and clones as models for mechanistic analysis of Natural Killer cell-mediated cytotoxicity. Cancer Res. 47: 2674.
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Vitale, M., Rizzoli, R., Mariani, A.R. et al. Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry. Cytotechnology 2, 59–62 (1989). https://doi.org/10.1007/BF00365415
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DOI: https://doi.org/10.1007/BF00365415