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Establishment of cell lines from serial samples from two patients with lung tumours before and after chemotherapy

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Abstract

Six cell lines were derived from pleural effusions of two lung cancer patients and established in vitro in our laboratory. Cell line AE1 was obtained from a small cell lung cancer (SCLC) before the patient had received any chemotherapy; the other lines (AE2 and AE3) were established from tumour recurrences in the same patient after therapy. Cell lines DG1 and DG2 were derived from specimens of an untreated non-small cell lung cancer (NSCLC), while cell line DG3 originated from pleural effusions recurring in the same patient after therapy. The results of the present study show that: (a) the SCLC lines AE1, AE2 and AE3 are heterogeneous in their biological characteristics and in their chemosensitivity patterns. In particular lines AE2 and AE3 are less responsive to cis-Platinum (DDP) and Adriamycin (ADM) than line AE1, so that they may reflect resistant subpopulations existing within the original tumour, selected following therapy with these drugs. In contrast, however, line AE1 proved more resistant to Vepesid (VP16) than lines AE2 and AE3. (b) The three NSCLC lines are similar in various biological features as well as in their chemosensitivity to DDP and Vinblastine (VBL).

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Abbreviations

NSCLC:

Non Small Cell Lung Cancer

SCLC:

Small Cell Lung Cancer

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Authors and Affiliations

  1. Laboratorio di Chemioterapia Sperimentale Preclinica, Istituto Regina Elena, Roma, Italy

    Nina Laudonio, Gabriella Zupi & Claudia Greco

  2. Sezione di Anatomia and Istopatologia e 38o Divisione di Pneumologia, Ospedale C. Forlanini, Roma, Italy

    Giuseppe Storniello

  3. Laboratory of Preclinical experimental chemotherapy, The Regina Elena Institute for Cancer Research, 291, v.le Regina Elena, 00161, Rome, Italy

    Franco Salvati

Authors
  1. Nina Laudonio
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  2. Gabriella Zupi
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  3. Giuseppe Storniello
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  4. Franco Salvati
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  5. Claudia Greco
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Recipient of a Fellowship of the Italian Association for Cancer Research

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Laudonio, N., Zupi, G., Storniello, G. et al. Establishment of cell lines from serial samples from two patients with lung tumours before and after chemotherapy. Cytotechnology 2, 39–47 (1989). https://doi.org/10.1007/BF00365413

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  • DOI: https://doi.org/10.1007/BF00365413

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