Abstract
Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.
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References
Duff RG (1985) Microencapsulation technology: a novel method for monoclonal antibody production. Tends in Biotechnology 3: 167–171.
Himmelfarb P, Thayer PS and Martin HE (1969) Spin filter culture: The propagation of mammalian cells in suspension. Science 164: 555–557.
Knazek RA, Gullino PM, Kohler PO and Dedrick RL (1972) Cell culture on artificial capillaries. An approach to tissue growth in vitro. Science 178: 65–67.
Lydersen K, Pugh G, Paris S, Sharma P and Noll A (1985) Ceramic matrix for large scale animal cell culture. Bio/Technology 3: 63–67.
Murakami H, Shimomura T, Nakamura T, Ohashi H, Shinohara K and Omura H (1984) Development of a basal medium for serum-free cultivation of hybridoma cells in high density. J. Agric. Chem. Soc. Japan, 58: 575–583.
Murakami H, Shimomura O, Ohashi H, Hashizume S, tokashiki M, Shinohara K, Yasumoto K, Nomoto K and Omura H (1985) Growth and differentiation of cells in defined environment: Serum-free stirred culture of human-human hybridoma lines. 111–116 copublished by Kodansha Ltd. and Springer-Verlag.
Tokashiki M, Hamamoto K, Takazawa Y and Ichikawa Y (1988) High-density culture of mouse-human hybridoma cells using a new perfusion culture vessel. Kagaku kogaku ronbunshu 14: 337–341.
Tolbert W and Feder J (1983) Large-scale cell culture technology. Annual Reports on Fermentation Process 6: 35–74.
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Tokashiki, M., Arai, T. High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium. Cytotechnology 2, 5–8 (1989). https://doi.org/10.1007/BF00365409
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DOI: https://doi.org/10.1007/BF00365409