Abstract
Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 μg/ml. One of the hybridoma cell lines was adapted to serum-free medium and maintained for 60 days in an automated hollow fiber system. During that time, 3 g of antibody was produced. Such yields make it possible to evaluate these monoclonals for their therapeutic potential in patients at risk for Pseudomonas infections.
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Gammon, M.C., Banas, M.H., Boccumini, L.E. et al. Heterohybridomas that secrete high levels of pseudomonas-specific therapeutic human monoclonal antibodies: their generation and large scale growth in an automated hollow fiber cell culture system. Cytotechnology 3, 51–60 (1990). https://doi.org/10.1007/BF00365266
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DOI: https://doi.org/10.1007/BF00365266