Abstract
The IM-9-P cell line is a variant of the human B-lymphoblastoid cell line IM-9 which ectopically secretes prolactin (hPRL). The heterogeneous line IM-9-P and three sublines of clonal origin, two of them positive and one negative for PRL gene expression, were subjected to cytogenetic analysis and compared with the reference line IM-9 which showed a normal female diploid karyotype. G-banding revealed several rearrangements in the chromosomes. Nine altered chromosomes including one stable marker chromosome were common to all analysed karyotypes of IM-9-P cells and their clones. A second marker chromosome ‘mar2’ occurred only in the karyotypes of the hPRL producing clones, but not in the non-producing clone. None of the visible alterations involve chromosome 6 which carries the PRL gene in humans.
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References
American Type Culture Collection Catalogue of cell lines and hybridomas, 5th edition (1985) Hay R, Macy M, Corman-Weinblatt A, Chen TR and McClintock P (ed.) ATCC, Rockville, Maryland.
Brøgger A (1982) The chromatid gap—a useful parameter in genotoxicology? Cytogenet. Cell Genet. 33: 14–19.
Caspersson T, Zech L, Johansson C and Modest EJ (1970) Identification of human chromosomes by DNA-binding fluorescent agents. Chromosoma 30: 215–227.
Chen TR, Hay RJ and Macy ML (1982) Karyotype consistency in human colorectal carcinoma cell lines established in vitro. Cancer Genet. Cytogenet. 6: 93–117.
Croce CM (1987) Role of chromosome translocations in human neoplasia. Cell 49: 155–156.
Croce CM and Nowell PC (1985) Molecular basis of human B cell neoplasia. Blood 65: 1–7.
Croce CM, Tsujimoto Y, Erikson J and Nowell P (1984) Biology of disease-Chromosome translocations and B cell neoplasia. Lab. Invest. 51: 258–267.
DiMattia GE, Gellersen B, Bohnet HG and Friesen HG (1988) A human B-lymphoblastoid cell line produces prolactin. Endocrinology 122: 2508–2517.
Emanuel BS, Selden JR, Chaganti RSK, Jhanwar S, Nowell PC and Croce CM (1984) The 2p breakpoint of a 2;8 translocation in Burkitt lymphoma interrupts the Vk locus. Proc. Natl. Acad. Sci. USA 81: 2444–2446.
Evans HJ and O'Riordan ML (1975) Human peripheral blood lymphocytes for the analysis of chromosome aberrations in mutagen tests. Mutat. Res. 31: 135–148.
Fahey JL, Buell DN and Sox HC (1971) Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann. N.Y. Acad. Sci. 190: 221–234.
Gebhart E (1988) What can the data of modern tumor cytogenetics teach us for mutagenicity testing? GUM 2: 2–8.
Gellersen B, DiMattia G, Kempf R and Bohnet HG (1989) Ectopically produced prolactin-an autocrine growth factor? Acta Endocrinologica 120 (Suppl. 1): 222.
ISCN (1985) An international system for human cytogenetic nomenclature, 1985, Karger, Basel.
Nilsson K and Klein G (1982) Phenotypic and cytogenetic characteristics of human B-lymphoid cell lines and their relevance for the etiology of Burkitt's lymphoma. Advances in Cancer Research 37: 319–380.
Oken MM (1984) Multiple myeloma. Medical Clinics of North America 68: 757–787.
Owerbach D, Rutter WJ, Cooke NE, Martial JA and Shows TB (1981) The prolactin gene is located on chromosome 6 in humans. Science 212: 815–816.
Pathak S (1986) Constitutional translocations and predisposition to T- and B-cell malignancies. Cytogenet. Cell Genet. 43: 223–225.
Philip P (1975) Marker chromosome 14q+ in multiple myeloma. Hereditas 80: 155–156.
Philip P and Drivsholm AA (1976) G-banding analysis of complex aneuploidy in multiple myeloma bone marrow cells. Blood 47: 69–77.
Rowley JD (1980) Chromosome abnormalities in human leukemia. Ann. Rev. Genet. 14: 17–39.
Seabright M (1971) A rapid banding technique for huma chromosomes. The Lancet 2: 971–972.
Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Expl. Cell. Res. 75: 304–306.
Van den Berghe H, Vermaelen K, Louwagie A, Criel A, Mecucci C and Vaerman J-P (1984) High incidence of chromosome abnormalities in IgG3 myeloma. Cancer Genet. Cytogenet. 11: 381–387.
Yunis JJ (1983) The chromosomal basis of human neoplasia. Science 221: 227–236.
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Bonhoff, A., Gellersen, B., Held, K.R. et al. Clonal derivatives of a human B-lymphoblastoid cell line producing prolactin—A cytogenetic characterization. Cytotechnology 3, 43–50 (1990). https://doi.org/10.1007/BF00365265
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DOI: https://doi.org/10.1007/BF00365265