Abstract
Process intensity of fixed bed glass sphere culture systems is increased considerably by replacing solid glass spheres with open pore glass spheres. This technique demonstrates the possibility of having a system capable of both volumetric and cell density scale up and being suitable for substrate attached and suspension cells. The yields achieved for a number of attached cell lines (approximately 107/ml) demonstrate an increase approaching one order of magnitude over solid glass spheres (approximately 106/ml). Also suspension cells were successfully entrapped in the open pore structure with similar yields.
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Looby, D., Griffiths, J.B. Fixed bed porous glass sphere (porosphere) bioreactors for animal cells. Cytotechnology 1, 339–346 (1988). https://doi.org/10.1007/BF00365079
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DOI: https://doi.org/10.1007/BF00365079