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The usefulness of CHAPS as a non-cytotoxic stabilizing agent in purification of growth factors

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Abstract

Among several detergents, a zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), was found to be least cytotoxic for cultured mammalian cells. CHAPS improved the activity recovery and elution profile of crude and purified fibroblast growth factors (FGFs) during chromatographies. Diluted preparations of FGFs were stabilized by CHAPS against the loss during storage. Amino acid sequence analysis was not disturbed by CHAPS. CHAPS was removable by reversed-phase high-performance liquid chromatography. These results indicate that CHAPS is useful as a non-cytotoxic stabilizing agent in purification of various kinds of bioactive polypeptides.

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Abbreviations

α-MEM:

Alpha Modification of Eagle's Minimal essential medium

CHAPS:

3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate

CHAPSO:

3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propane sulfonate

CS:

Calf Serum

EGF:

Epidermal Growth Factor

FGF:

Fibroblast Growth Factor

HPLC:

High-Performance Liquid Chromatography

NGF:

Nerve Growth Factor

NOG:

1-O-n-octyl-β-D-glucopyranoside

NP-40:

Nonidet P-40

PBS:

Phosphate-Buffered Saline

SB 12:

3-(dodecylmethylammonio)-1-propane sulfonate

SDS:

Sodium Dodecyl Sulfate

TGF-α and β:

Transforming Growth Factor type α and β

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Authors and Affiliations

  1. Department of Endocrinology, Kagawa Medical School, 1750, Miki-cho, Kita-gun, 761-07, Kagawa, Japan

    Yuhsi Matuo, Nozomu Nishi, Yasuyoshi Muguruma & Fumio Wada

  2. Department of Biochemistry, Kanazawa Medical University Uchinada, 920-02, Ishikawa, Japan

    Yoshino Yoshitake, Yoshishige Masuda & Katsuzo Nishikawa

Authors
  1. Yuhsi Matuo
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  2. Nozomu Nishi
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  3. Yasuyoshi Muguruma
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  4. Yoshino Yoshitake
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  5. Yoshishige Masuda
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  6. Katsuzo Nishikawa
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  7. Fumio Wada
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Matuo, Y., Nishi, N., Muguruma, Y. et al. The usefulness of CHAPS as a non-cytotoxic stabilizing agent in purification of growth factors. Cytotechnology 1, 309–318 (1988). https://doi.org/10.1007/BF00365076

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