Abstract
The hormones present in the water extract of the eyestalks of Crangon crangon (L.) were separated by gel filtration on Sephadex G-25 fine in 0.1 M acetic acid. Chromatophorotropic activity was present in 3 separate fractions. Peak II (Fig. 1) contained Black Pigment Dispersing Hormone (BPDH); a concentrating activity, especially towards the pigment of leucophores, was present in fraction IIIA (WPCH). Peak IV contained Red Pigment Concentrating Hormone (RPCH). Combining BPDH with RPCH in the ratio 5:1, effects of lightening of the body and darkening of the telson and uropods were observed. A similar effect was produced when BPDH and WPCH were combined in the ratio 1:1. Such effects have not been previously described for eyestalk hormones, but only for the hormones of the tritocerebral commissure. In the crude water extract of the eyestalk, BPDH is inhibited by the remaining 2 hormones (WPCH and RPCH), and shows no dispersing effect when tested on eyestalkless C. crangon.
Similar content being viewed by others
Literature cited
Bartell, C. K. and D. J. Fluke: Temperature dependence of ionizing radiation effect on dry preparations of two melanophore-stimulating hormones. Radiat. Res. 30, 811–824 (1967).
Brown, F. A., Jr.: Hormones in crustaceans. In: The actions of hormones in plants and invertebrates, pp 171–214. Ed. by K. V. Thimann. New York: Academic Press 1952.
—Chromatophores and color change. In: Comparative animal physiology, pp 502–537. Ed. by C. L. Prosser. Philadelphia: W. B. Saunders Co. 1962.
—J. R. Klotz: Separation of two mutually antagonistic chromatophorotropins from the tritocerebral commissure of Crangon. Proc. Soc. exp. Biol. Med. 64, 310–313 (1947).
—V. J. Wulff: Chromatophore types in Cragon and their endocrine control. J. cell. comp. Physiol. 18, 339–353 (1941).
Carlisle, D. B. and F. Knowles: Endocrine control in crustaceans, 120 pp. Cambridge: University Press 1959.
Fingerman, M.: The control of chromatophores, 184 pp. New York: Pergamon Press Inc. 1963.
Gersch, M.: Vergleichende Endokrinologie der wirbellosen Tiere, 535 pp. Leipzig: Akademische Verlagsgesellschaft 1964.
Hogben, L. T. and D. Slome: The pigmentary effector system. VI. The dual character of endocrine coordination in amphibian colour change. Proc. R. Soc. (Ser. B) 108, 10–53 (1931).
Josefsson, L.: Separation and purification of distal retinal pigment hormone and red pigment concentrating hormone of the crustacean eyestalk. Biochim. biophys. Acta 148, 300–303 (1967).
Kleinholz, L. H.: Pigmentary effectors. In: The physiology of Crustacea, Vol. II, pp 133–169. Ed. by T. H. Waterman, New York: Academic Press 1961.
—F. Kimball: Separation of neurosecretory pigmentary-effector hormones of the crustacean eyestalk. Gen. Comp. Endocr. 5, 336–341 (1965).
Sandeen, M. J.: Chromatophototropins in the central nervous system of Uca pugilator, with special reference to their origins and actions. Physiol. Zoöl. 23, 337–352 (1950).
Author information
Authors and Affiliations
Additional information
Communicated by F. Pautsch, Gdańsk-Wrzeszcz
Rights and permissions
About this article
Cite this article
Skorkowski, E.F. Isolation of three chromatophorotropic hormones from the eyestalk of the shrimp Crangon crangon . Marine Biology 8, 220–223 (1971). https://doi.org/10.1007/BF00355219
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00355219