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A study of the cement apparatus of the cypris larva of the barnacle Balanus balanoides

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Abstract

Two types of secretory cell have been found in the cement glands of the cypris larva of Balanus balanoides (L.). Histochemical tests show the presence of proteins, phenols and the enzyme polyphenol oxidase in both the glands and secreted cement. It is proposed that cyprid cement is composed of tanned protein, all the components of which are derived from the cement glands.

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Literature cited

  • Barnes, H.: Further observations on rugophilic behaviour in Balanus balanoides (L.). Vidensk. Meddr dansk. naturh. Foren. 117, 341–348 (1955).

    Google Scholar 

  • Barrnett, R. J. and A. M. Seligman: Demonstration of protein bound sulphydryl and disulphide groups by two new histochemical methods. J. natn. Cancer Inst. 94, 176–183 (1952).

    Google Scholar 

  • Brown, C. H.: Quinone tanning in the animal kingdom. Nature. Lond. 165, p. 275 (1950).

    Google Scholar 

  • Casselman, W. G. B.: Histochemical technique, pp 31–44. London: Methuen & Co. Ltd. 1962.

    Google Scholar 

  • Crisp, D. J.: The behaviour of barnacle cyprids in relation to water movements over a surface. J. exp. Biol. 32, 568–590 (1955).

    Google Scholar 

  • —: Territorial behaviour in barnacle settlement. J. exp. Biol. 38, 429–446 (1961).

    Google Scholar 

  • — and H. Barnes: The orientation and distribution of barnacles at settlement with particular reference to surface contour. J. Anim. Ecol. 23, 142–162 (1954).

    Google Scholar 

  • — and P. S. Meadows: The chemical basis of gregariousness in cirripedes. Proc. R. Soc. (B.) 156, 500–520 (1962).

    Google Scholar 

  • —: Absorbed layers: the stimulus to settlement in barnacles. Proc. R. Soc. (B.) 158, 364–387 (1963).

    Google Scholar 

  • Darwin, C.: A monograph of the sub-class Cirripedia: Balanidae, Verrucidae, etc., pp 110–119. London: Ray Society 1854.

    Google Scholar 

  • Doochin, H. D.: The morphology of Balanus improvisus Darwin and Balanus amphitrite niveus Darwin during initial attachment and metamorphosis. Bull. mar. Sci. Gulf Caribb. 1, 15–39 (1951).

    Google Scholar 

  • Gabbott, P. A. and V. N. Larman: Electrophoretic examination of partially purified extracts from Balanus balanoides containing a settlement inducing factor. Proc. IV European Mar. Biol. (1971). (In press).

  • Gibson, P. H. and J. A. Nott: Concerning the fourth antennular segment of the cypris larva of Balanus balanoides. Proc. IV European Mar. Biol. Symp. (1971). (In press).

  • Harris, J. E.: Report on anti-fouling research 1942–44. J. Iron Steel Inst. 2, 297–333 (1946).

    Google Scholar 

  • Hillman, R. E. and P. F. Nace: Adhesion in biological systems, pp 113–121. Ed. by R. S. Manly. New York: Academic Press 1970.

    Google Scholar 

  • Knight-Jones, E. W.: Laboratory experiments on gregariousness during setting in Balanus balanoides and other barnacles. J. exp. Biol. 30, 584–598 (1953).

    Google Scholar 

  • — and D. J. Crisp: Gregariousness in barnacles in relation to the fouling of ships and to antifouling research. Nature, Lond. 171, p. 1109 (1953).

    Google Scholar 

  • — and J. Moyse: Intraspecific competition in sedentary marine animals. Symp. Soc. exp. Biol. 15, 72–95 (1961).

    Google Scholar 

  • Kramer, H. and G. M. Windrum: The metachromatic staining reaction. J. Histochem. Cytochem. 3, 227–237 (1955).

    Google Scholar 

  • Laskey, A. M.: A modification of Mayer's mucihaematin technique. Stain Technol. 25, 33–34 (1950).

    Google Scholar 

  • Lison, L.: Histochimie animale. In: Méthodes et problèmes, pp 139–160. Paris: Gautier-Villars 1936.

    Google Scholar 

  • Mazia, D., P. A. Brewer and M. Aleeat: The cytochemical staining and measurement of protein with mercuric bromophenol blue. Biol. Bull. mar. biol. Lab., Woods Hole 104, 57–67 (1953).

    Google Scholar 

  • Nott, J. A.: Settlement of barnacle larvae: surface structure of the antennular attachment disc by scanning electron microscopy. Mar. Biol. 2, 248–251 (1969).

    Google Scholar 

  • — and B. A. Foster: On the structure of the antennular attachment organ of the cypris larva of Balanus balanoides (L.). Phil. Trans. R. Soc. (B.) 256, 115–134 (1969).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, theoretical and applied, 2nd Ed., London: J.& A. Churchill Ltd. 1960.

    Google Scholar 

  • Pease, D. C.: Histochemical techniques for electron microscopy, 2nd Ed., pp 37–41. New York: Academic Press 1964.

    Google Scholar 

  • Pryor, M. G. M.: Comparative biochemistry, Vol 4B, pp 371–396, Ed. by M. Florkin and H. S. Mason. New York: Academic Press 1962.

    Google Scholar 

  • Saroyan, J. R., Lindner, E., Dooley, C. A. and H. R. Bleile: Barnacle cement — key to second generation antifouling coatings. Ind. Engng Chem. prod. Res. Dev. 9 (2), 122–133 (1970).

    Google Scholar 

  • Shimizu, N. and R. Kumamoto: A lead tetra-acetate schiff method for polysaccharides in tissue sections. Stain Technol. 27, 97–106 (1952).

    Google Scholar 

  • Steedman, H. F.: Alcianblue 8GS: a new stain for mucin. Q. Jl microsc. Sci. 91, 477–479 (1950).

    Google Scholar 

  • Smyth, J. D.: A technique for the histochemical demonstration of polyphenol oxidase and its application to eggshell formation in helminths and byssus formation in Mytilus. Q. Jl microsc. Sci. 95, 139–152 (1954).

    Google Scholar 

  • Visscher, J. P.: Reactions of the cyprid larvae of barnacles at the time of attachment. Biol. Bull. mar. biol. Lab., Woods Hole 54, 327–335 (1928).

    Google Scholar 

  • Walley, L. J.: Studies on the larval structure and metamorphosis of Balanus balanoides (L.). Phil. Trans. R. Soc. (B.) 256, 237–280 (1969).

    Google Scholar 

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Communicated by J. H. S. Blaxter, Oban

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Walker, G. A study of the cement apparatus of the cypris larva of the barnacle Balanus balanoides . Marine Biology 9, 205–212 (1971). https://doi.org/10.1007/BF00351380

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