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c-Fos protooncogene expression in human normal peripheral blood leukocytes

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Abstract

The aim of this study was to investigate the expression of c-fos protooncogene in normal human peripheral blood leukocytes by Northern blot analysis. Unlike lymphoid cells, monocytes express appreciable levels of c-fos transcripts. Unexpectedly, unlike HL-60 (promyelocytic)-derived granulocytes, freshly isolated polymorphonuclear cells (PMN) showed c-fos expression, with higher levels of expression then monocytes. The expression of c-fos by PMN was superinduced by cycloheximide. When PMN were treated with stimuli known to activate some functional activities of these cells (phorbol esters, inactivated streptococci, colony-stimulating factors, tumor necrosis factor) the expression of c-fos was augmented. The ‘constitutive’ and inducible expression of c-fos in PMN was also detectable after 20 hours of culture. These data show that, among the cells belonging to the myelo-monocytic lineage, c-fos expression is not restricted to monocytes-macrophages and that data obtained from established cell lines are not necessarily representative of fresh normal cells. c-fos expression in PMN may play a role in the regulation of mature circulating myelocytes.

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Abbreviations

CH:

Cycloheximide

G- and GM-CSF:

granulocyte and granulocytemacrophage colony stimulating factor

PMA:

13 Phorbol-12-Myristate Acetate

PMN:

Polymorphonuclear Cells

TNF:

Tumor Necrosis Factor

References

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Colotta, F., Polentarutti, N., Wang, J.M. et al. c-Fos protooncogene expression in human normal peripheral blood leukocytes. Cytotechnology 1, 61–64 (1987). https://doi.org/10.1007/BF00351124

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  • DOI: https://doi.org/10.1007/BF00351124

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