Skip to main content
SpringerLink
Log in

Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing

  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

Individual chromosomes can be identified by means of in situ hybridization with DNA probes for chromosome-specific repetitive sequences. The efficiency and sensitivity of the method are strictly dependent on the characteristics of the probes and the experimental conditions. Using three probes with different copy numbers, we demonstrated that the target chromosomes can be visualized in interphase when the homologous sequences are repeated at least 50 times.

Possible applications of interphase analysis to clinical cytogenetics and mutagenicity testing are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cremer T, Landegent J, Brukner A, Scholl HP, Schardin M, Hager HD, Devilee P, Pearson P and Van derPoleg M (1986) Detection of chromosome aberrations in the human interphase nucleus, by visualization of specific target DNAs with radioactive and nonradioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L 1.84. Hum. Genet. 74: 346–352.

    Google Scholar 

  2. Davies KE, Young BD, Elles RG, Hill ME and Williamson R (1981) Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry. Nature 293: 374–376.

    Google Scholar 

  3. Harper ME and Saunders GF (1981) Localization of single copy DNA sequences on G banded human chromosomes by in situ hybridization. Chromosoma 83: 431–439.

    Google Scholar 

  4. Jeanpierre M, Weil D, Gallano P, Creau-Goldberg N and Junien C (1985) The organization of two related subfamilies of a human tandemly repeated DNA is chromosome-specific. Hum. Genet. 70: 302–310.

    Google Scholar 

  5. Mattei MG, Philip N, Passage E, Moisan JP, Mandel JL and Mattei JF (1985) DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome. Hum. Genet. 69: 268–271.

    Google Scholar 

  6. Oberlé I, Heiling R, Moisan JP, Kloepfer C, Mattei MG, Mattei JF, Boné J, Froster-Iskenius U, Jacobs PA, Tathrop GM, Talance JM and Mandel JL (1986) Genetic analysis of the fragile X mental retardation syndrome with two flanking polymorphic DMA markers. Proc. Natl. Acad. Sci. USA 83: 1016–1020.

    Google Scholar 

  7. Raimondi E, Scariolo S, DeSario A, and DeCarli L (1986) Aneuploidy assays on interphase nuclei by means of in situ hybridization with DNA probes. Eur. J. Cell Biol. 42 (s16): 4.

    Google Scholar 

  8. Rappold GA, Cremer T, Hager HD, Davies KE, Muller CR and Yang T (1984) Sex chromosomes position in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probe. Hum. Genet. 67: 317–325.

    Google Scholar 

  9. Wolfe J, Darling SM, Erickson RP, Craig JW, Buckle VL, Rigby PWJ, Willard HF and Goodfellow PN (1985) Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome. J. Mol. Biol. 182: 477–485.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Genetica e Microbiologia ‘A. Buzzati-Traverso’, Univ. di Pavia, Via S. Epifanio 14, 27100, Pavia, Italy

    E. Raimondi, S. Scariolo, P. Vagnarelli, A. De Sario & L. De Carli

Authors
  1. E. Raimondi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Scariolo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Vagnarelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. De Sario
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. De Carli
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Raimondi, E., Scariolo, S., Vagnarelli, P. et al. Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing. Cytotechnology 1, 13–17 (1987). https://doi.org/10.1007/BF00351116

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00351116

Key words

Search

Navigation