Skip to main content
SpringerLink
Log in

Construction of baculovirus recombinants

  • Published:
Cytotechnology Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

AcMNPV:

Autographa californica multinucleocapsid nuclear polyhedrosis virus

SDS:

Sodium dodecyl sulphate

PCR:

polymerase chain reaction

References

  • AbremskiK, HoessR & SternbergN (1983) Studies on the properties of P1 site-specific recombination: evidence for topologically unlinked products following recombination. Cell 32: 1301–1311.

    Google Scholar 

  • AyresMD, HowardSC, KuzioJ, Lopez-FerberM & PosseeRD (1994) The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202: 586–605.

    Google Scholar 

  • BonningBC & HammockBD (1992) Development and potential of genetically engineered viral insecticides. In: TombsMP (ed.) Biotechnology and genetic engineering reviews, Vol. 10 (pp. 455–489) Intercept, Andover.

    Google Scholar 

  • ClemRJ, FechheimerM & MillerLK (1991) Prevention of apoptosis by a baculovirus gene during infection of insect cells. Science 254: 1388–1390.

    Google Scholar 

  • CopelandWC & WangTS-F (1993) Mutational analysis of the human DNA polymerase α. J. Biol. Chem. 268: 11028–11040.

    Google Scholar 

  • DayA, WrightT, SewallA, Price-LafaceM, SrivastavaN & FinlaysonM (1995) Rapid procedures for the isolation and PCR analysis of recombinant baculovirus. In: Richardson (ed.) Methods in Molecular Biology, Vol. 39: Baculovirus Expression Protocols (pp. 143–159) Humana Press, Totowa, NJ.

    Google Scholar 

  • DaviesAH (1994) Current methods for manipulating baculoviruses. Bio/Technology 12: 47–50.

    Google Scholar 

  • ErnstWJ, GrabherrRM & KatingerHWD (1994) Direct cloning into the Autographa californica nuclear polyhedrosis virus for generation of recombinant baculoviruses. Nucleic Acids Res. 22: 2855–2856.

    Google Scholar 

  • FungM-C, ChiuKYM, WeberT, ChangT-W & ChangNT (1988) Detection and purification of a recombinant human B lymphotropic virus hhv-6 in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization. J. Virol. Meth. 19: 33–42.

    Google Scholar 

  • GodeauF, SaucierC & KourilskyP (1992) Replication inhibition by nucleoside analogues of a recombinant Autographa californica multicapsid nuclear polyhedrosis virus harboring the herpes thymidine kinase gene driven by the IE-1(0) promoter: a new way to select recombinant baculoviruses. Nucleic Acids Res. 20: 6239–6246.

    Google Scholar 

  • GranadosRR & FedericiBA (1986) The biology of baculoviruses, Vol. II. CRC Press, Boca Raton, Florida.

    Google Scholar 

  • GrosseF & MannsA (1995) Fast isolation of recombinant baculovirus by antibody screening. In: Richardson (ed.) Methods in Molecular Biology, Vol. 39: Baculovirus Expression Protocols (pp. 179–185). Humana Press, Totowa, NJ.

    Google Scholar 

  • HartigPC & CardonMC (1992) Rapid efficient production of baculovirus expression vectors. J. Virological Methods 38: 61–70.

    Google Scholar 

  • HershbergerPA, DicksonJA & FriesenPD (1992) Site-specific mutagenesis of the 35-kilodalton protein gene encoded by Autographa californica nuclear polyhedrosis virus: cell line-specific effects on virus replication. J. Virol. 66: 5525–5533.

    Google Scholar 

  • HoessR & AbremskiK (1984) Interaction of the bacteriophage P1 recombinase Cre with the recombining site loxP. Proc. Natl. Acad. Sci. USA 81: 1026–1029.

    Google Scholar 

  • KittsPA (1995) Production of recombinant baculoviruses using linearized viral DNA. In: Richardson (ed.) Methods in Molecular Biology, Vol. 39: Baculovirus Expression Protocols (pp. 129–142) Humana Press, Totowa, NJ.

    Google Scholar 

  • KittsPA, AyresMD & PosseeRD (1990) Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res. 18: 5667–5672.

    Google Scholar 

  • KittsPA & PosseeRD (1993) A method for producing recombinant baculovirus expression vectors at high frequency. BioTechniques 14: 810–817.

    Google Scholar 

  • LalumièreM & RichardsonCD (1995) Production of recombinant baculoviruses using rapid screening vectors that contain the gene for β-galactosidase. In: Richardson (ed.) Methods in Molecular Biology, Vol. 39: Baculovirus Expression Protocols (pp. 161–177) Humana Press, Totowa, NJ.

    Google Scholar 

  • LerchRA & FriesenPD (1993) The 35-kilodalton protein gene (p35) of Autographa californica nuclear polyhedrosis virus and the neomycin resistance gene provide dominant selection of recombinant baculoviruses. Nucleic Acids Res. 21: 1753–1760.

    Google Scholar 

  • López-FerberM, SiskWP & PosseeRD (1995) Baculovirus transfer vectors. In: Richardson (ed.) Methods in Molecular Biology, Vol. 39: Baculovirus Expression Protocols (pp. 25–63) Humana Press, Totowa, NJ.

    Google Scholar 

  • LuckowVA, LeeSC, BarryGF & OlinsPO (1993) Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J. Virol. 67: 4566–4579.

    Google Scholar 

  • MannsA & GrosseF (1991) Fast isolation of recombinant baculovirus by antibody screening. BioTechniques 10: 154–158.

    Google Scholar 

  • MartensJWM, vanOersMM, van deBiltBD, OudshoornP & VlakJM (1995) Development of a baculovirus vector that facilitates the generation of p10-based recombinants. J. Virological Meths. 52: 15–19.

    Google Scholar 

  • PatelG, NasmythK & JonesN (1992) A new method for the isolation of recombinant baculovirus. Nucleic Acids Res. 20: 97–104.

    Google Scholar 

  • PeakmanT, PageM & GewertD (1989) Increased recombinational efficiency in insect cells irradiated with short wavelength ultraviolet light. Nucleic Acids Res. 17: 5403.

    Google Scholar 

  • PeakmanTC, HarrisRA & GewertDR (1992) Highly efficient generation of recombinant baculoviruses by enzymatically mediated site-specific in vitro recombination. Nucleic Acids Res. 20: 495–500.

    Google Scholar 

  • PenJ, WellingGW & Welling-WesterS (1989) An efficient procedure for the isolation of recombinant baculovirus. Nucleic Acids Res. 17: 451.

    Google Scholar 

  • PengS, SommerfeltMA, BertaG, BerryAK, KirkKL, HunterE & SorscherEJ (1993) Rapid purification of recombinant baculovirus using fluorescence-activated cell sorting. Bio Techniques 14: 274–277.

    Google Scholar 

  • PennockGD, ShoemakerC & MillerLK (1984) Strong and regulated expression of Escherichia coli β-galactosidase in insect cells with a baculovirus vector. Mol. Cell. Biol. 4: 399–406.

    Google Scholar 

  • PhamDQ-D, HiceRH, Sivasubramanian & FedericiBA (1993) The 1629-bp open reading frame of the Autographa californica multinucleocapsid nuclear polyhedrosis virus encodes a virion structural protein. Gene 137: 275–280.

    Google Scholar 

  • PosseeRD, SunT-P, HowardSC, AyresMD, Hill-PerkinsM & GearingKL (1991) Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4 kbp EcoRI-I and-R (polyhedrin gene) region. Virology 185: 229–241.

    Google Scholar 

  • RichardsonC, AttiaJ, DunnR, GuptaS, O'ConnorM, SemeniukD, TamJ, HamelM, LambertG, DennisM, JacobsF, MartinL, IorioC & VialardJ (1992a) Engineering glycoproteins for secretion using the baculovirus expression system. In: VlakJM, SchlaegerE-J & BernardAR (eds) Baculoviruses and recombinant protein production processes (pp. 67–74) Roche, Basel.

    Google Scholar 

  • RichardsonCD, BanvilleM, LalumièreM, VialardJ & MeighenEA (1992b) Bacterial luciferase produced with rapid screening baculovirus vectors is a sensitive reporter for infection of insect cells and larvae. Intervirology 34: 213–227.

    Google Scholar 

  • SmithGE, FraserMJ & SummersMD (1983) Molecular engineering of the Autographa californica nuclear polyhedrosis virus genome: deletion mutations within the polyhedrin gene. J. Virol. 46: 584–593.

    Google Scholar 

  • SternbergN & HoessR (1983) The molecular genetics of bacteriophage P1. Ann. Rev. Genet. 17: 123–154.

    Google Scholar 

  • Summers MD & Smith GE (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin No. 1555.

  • VialardJ, LalumièreM, VernetT, BriedisD, AlkhatibG, HenningD, LevinD & RichardsonC (1990) Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the β-galactosidase gene. J. Virol. 64: 37–50.

    Google Scholar 

  • VialardJE & RichardsonCD (1993) The 1,629-nucleotide open reading frame located downstream of the Autographa californica nuclear polyhedrosis virus polyhedrin gene encodes a nucleocapsid-associated phosphoprotein. J. Virol. 67: 5859–5866.

    Google Scholar 

  • VlakJM, SchoutenA, UsmanyM, BelshamGJ, Klinge-RoodeEC, MauleAJ, vanLentJWM & ZuidemaD (1990) Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. Virology 179: 312–320.

    Google Scholar 

  • WeyerU, KnightS & PosseeRD (1990) Analysis of very late gene expression by Autographa californica nuclear polyhedrosis virus and the further development of multiple expression vectors. J. Gen. Virol. 71: 1525–1534.

    Google Scholar 

  • WoodHA & GranadosRR (1991) Genetically engineered baculoviruses as agents for pest control. Ann. Rev. Microbiol. 45: 69–87.

    Google Scholar 

  • ZuidemaD, SchoutenA, UsmanyM, MauleAJ, BelshamGJ, RoosienJ, Klinge-RoodeEC, vanLentJWM & VlakJM (1990) Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector. J. gen. Virol. 71: 2201–2209.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. CLONTECH Laboratories, Inc., 1020 East Meadow Circle, 94303-4230, Palo Alto, CA, U.S.A.

    Paul A. Kitts

Authors
  1. Paul A. Kitts
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kitts, P.A. Construction of baculovirus recombinants. Cytotechnology 20, 111–123 (1996). https://doi.org/10.1007/BF00350392

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00350392

Key words

Search

Navigation