Conclusion
Current methods of insect cell culture have produced a limited variety of cell types in an ever expanding list of insect cell lines. In developing midgut epithelial cell lines, we found that traditional methods in insect cell culture failed to provide healthy cells from mature tissues. Examination of mammalian cell culture literature for this particular cell type provided the insight required to successfully develop a cell-specific line (Baines et al., 1994). The potential applications for cell-specific lines from insects are numerous. This paper is a compilation of ideas that will hopefully enable other researchers to develop additional cell-specific lines.
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References
AidleyDL (1985) Muscular contraction. In: KerkutGA & GilbertLI (eds) Comprehensive Insect Physiology Biochemistry and Pharmacology. Vol. 5 (pp. 407–438), Pergamon Press, New York.
AloiLE & CherryRS (1994) Intracellular calcium response of Sf-9 insect cells exposed to intense fluid forces. J. Biotechnol. 33: 21–31.
BainesD, BrownwrightA, SchwartzJ-L (1994) Establishment of primary and continuous cultures of midgut epithelial cells of the spruce budworm, gypsy moth and silkworm. J. Insect Physiol. 40: 347–357.
BainesD, DesantisT & DownerRGH (1992) Octopamine and 5-hydroxytryptamine enhance the phagocytic and nodule formation activities of cockroach (Periplaneta americana) haemocytes. J. Insect Physiol. 38: 905–914.
BainesRA & DownerRGH (1991) Pharmacological characterization of a 5-hydroxytryptamine-sensitive receptor/adenylate cyclase complex in the mandibular closer muscles of the cricket, Gryllus domestica. Archs. Insect Biochem. Physiol. 16: 153–163.
BeadleDJ & HicksD (1985) Insect nerve culture. In: KerkutGA & GilbertLI (eds) Comprehensive Insect Physiology Biochemistry and Pharmacology. Vol. 5 (pp. 181–211) Pergamon Press, New York.
BergerEM, FrankM & AbellMC (1980) Ecdysone-induced changes in protein synthesis in embryonic Drosophila cells in vitro. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 195–208) Elsevier/North-Holland Biomedical Press, New York.
BrooksMA, TsangKR & FreemanFA (1980) Cholesterol as a growth factor for insect cell lines. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 67–78) Elsevier/North-Holland Biomedical Press, New York.
ChewCS (1994) Parietal cell culture: new models and directions. Annu. Rev. Physiol. 56: 445–61.
CherbasL, CherbasP, SavakisC, DemetriG, Manteuffel-CymborowskaM, YoungeCD & WilliamsCM (1980) Studies of ecdysteroid action on a Drosophila cell line. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 217–228) Elsevier/North-Holland Biomedical Press, New York.
CoastGM (1989) Stimulation of fluid secretion by single isolated malpighian tubules of the house cricket, Acheta domesticus. Physiol. Entomol. 14: 21–30.
CourgeonAM, RoppM, RolletE, BeckerJ, MaisonhauteC, EchalierG & Best-BelpommeM (1989) Relationships between ecdysterone-induced cellular differentiation and aerobiosis in an in vitro Drosophila cell system. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 1 (pp. 93–97) CRC Press Inc, Florida.
DavisTR, WickhamTJ, McKennaKA, GranadosRR, ShulerML & WoodHA (1993) Comparative recombinant protein production of eight insect cell lines. In Vitro Cell. Dev. Biol. 29A: 388–390.
DownerRGH (1979) Trehalose production in isolated fat body of the American cockroach, Periplaneta americana. Comp. Biochem. Physiol. 62C: 31–34.
DownerRGH & HiripiL (1993) Biogenic amines in insects. In: BorkovecAB & LoebMJ (eds) Insect neurochemistry and neurophysiology. (pp. 23–38) CRC press, Ann Arbor.
DeutschmanDM & JagerV (1994) Optimization of the growth conditions of Sf21 insect cells for high-density perfusion culture in stirred-tank bioreactors. Enzyme Microb. Technol. 16: 506–512.
EchalierG (1980) Necessity of radically new insect cell culture methods. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 589–592) Elsevier/North-Holland Biomedical Press, New York.
EnglishLH, MagelkyBK & MarksEP (1984) 20-Hydroxyecdysone-induced changes in the cell volume of lepidopteran cells associated with population dynamics. In Vitro 20: 71–78.
EvansGS, FlintN & PottenCS (1994) Primary cultures for studies of cell regulation and physiology in intestinal epithelium. Annu. Rev. Physiol. 56: 399–417.
FreshneyRI (1992) Introduction. In: FreshneyRI (ed) Culture of Epithelial Cells. (pp. 1–23) Wiley-Liss, New York.
FreshneyRH (1994) Culture of animal cells. Wiley-Liss, New York.
FusenigNE (1992) Cell interaction and epithelial differentiation. In: FreshneyRI (ed) Culture of Epithelial Cells. (pp. 25–57) Wiley-Liss, New York.
GardinerGR & StockdaleH (1975) Two tissue culture media for production of Lepidopteran cells and nuclear polyhedrosis viruses. J. Invert. Pathol. 25: 363–370.
GelernterWD & FedericiBA (1986) Continuous cell line from Spodoptera exigua (Lepidoptera: Noctuidae) that supports replication of nuclear polyhedrosis viruses from Spodoptera exigua and Autographa californica. J. Invert. Path. 48: 199–207.
GraceTDC (1962) Establishment of four strains of cells from insect tissues grown in vitro. Nature 195: 788–789.
GoleJWD, DownerRGH & SohiSS (1982) Octopamine-sensitive adenylate cyclase in haemocytes of the forest tent caterpillar, Malacosoma disstria Hubner (Lepidoptera: Lasiocampidae). Can. J. Zool. 60: 825–829.
GoodwinRH & AdamJR (1980) Nutrient factors influencing viral replication in serum-free insect cell line culture. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 493–510) Elsevier/North-Holland Biomedical Press, New York.
Goodwin RH (1985) Growth of insect cells in serum-free media. Techniques in the Life Sciences: Setting Up and Maintenance of Tissue and Cell Cultures C(109): 1–28.
GoodwinRH (1989) Construction of peptoliposomes for the incorporation of nutrient lipid supplements in insect cell culture medium. J. Tissue Culture Methods 12: 17–20.
GranadosRR & HashimotoY (1989) Infectivity of baculoviruses to cultured cells. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 2 (pp. 3–13) CRC Press Inc, Florida.
GreeneAE, CharneyJ, NicholsWW & CoriellLL (1972) Species identity of insect cell lines. In Vitro 5: 313–322.
HarveyGT & SohiSS (1989) Isozyme characterization of 8 Hymenopteran and 20 Lepidopteran Cell lines. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 1 (pp. 71–76) CRC Press Inc, Florida.
HinkWF (1980) The 1979 compilation of invertebrate cell lines and culture media. In. Invertebrate Systems in vitro. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 553–578) Elsevier/North-Holland Biomedical Press New York.
HinkWF & BezansonDR (1985) Invertebrate cell culture media and cell lines. Techniques in the Life Sciences: Setting Up and Maintenance of Tissue and Cell Cultures. C111: 1–30.
HinkWF & HallRL (1989) Recently established cell lines. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 2 (pp. 269–293) CRC Press Inc, Florida.
HinkWF & StraussEM (1980) Semi-continuous culture of the TN-368 cell line in fermenters with virus production in harvested cells. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 27–34) Elsevier/North-Holland Biomedical Press, New York.
HouseCR & GinsbergBL (1979) Pharmacology of cockroach salivary secretion. Comp. Biochem. Physiol. 63C: 1–6.
JohnsonDE (1994) Cellular toxicities and membrane binding characteristics of insecticidal crystal proteins from Bacillus thuringiensis toward cultured insect cells. J. Invert. Pathol. 63: 123–129.
KurodaY & ShimadaY. (1989) Electron microscopic studies on in vitro differentiated cells from Drosophila embryos. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 1 (pp. 77–89) CRC Press Inc, Florida.
KurttiTJ & BrooksMA (1977) Isolation of cell lines from embryos of the cockroach, Blattella germanica. In Vitro 13: 11–17.
KurttiTJ & MunderlohUG (1989) Advances in the definition of culture media for mosquito cells. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 1 (pp. 21–30) CRC Press Inc, Florida.
LeeSH & ParkTH (1994) Growth limiting factors influencing high density culture of insect cells in Grace's medium. Biotechnol. Lett. 16: 327–332.
LoebMJ (1991a) Development of isolated spermducts from Heliothis virescens (Lepidoptera)in vitro. Invert. Reprod. Develop. 21: 67–73.
LoebMJ (1991b) Growth and development of spermducts of the tobacco budworm moth Heliothis virescens, in vivo and in vitro. Invert. Reprod. and Dev. 19: 97–105.
LoebMJ, BrandtEP & WoodsCW (1989) Testes of the tobacco budworm moth: ecdysteroid production by the testis sheath. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 1 (pp. 105–110) CRC Press Inc, Florida.
LynnDE (1989) Methods for the development of cell lines from insects. J. Tissue Culture Methods 12: 23–29.
LynnDE & OberlanderH (1989) Characterization of cell lines derived from imaginal disks of three species of Lepidoptera. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 2 (pp. 207–212) CRC Press Inc, Florida.
LynnDE, FeldlauferMF & LusbyWR (1987) Isolation and identification of 20-hydroxyecdysone from a Lepidopteran continuous cell line. Arch. Insect Biochem. Physiol. 5: 71–79.
McIntoschAH, EversD & ShamyR (1976) A toxic substance in fetal bovine serum (abstract). In Vitro 12: 302.
MitsuhashiJ (1989) Nutritional requirements of insect cells in vitro. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 1 (pp. 3–20) CRC Press Inc, Florida.
MitsuhashiJ & GoodwinRH (1989) Serum-free cultures of insect cells in vitro. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 1 (pp. 31–44) CRC Press Inc, Florida.
MitsuhashiJ & ShozawaA (1985) Continuous cell lines from larval haemocytes of the cabbage armyworm, Mamestra brassicae. Develop. Growth and Differ. 27: 599–60
MordueW, GoldsworthyGJ, BradyJ & WMBlaney (1980) Insect Physiology, Blackwell Scientific Publications, Oxford.
NoriegaFG & WellsWA (1992) Oxygen-carrying perfluorochemical emulsions improve insect fat body culture performance. Insect Biochem. Molec. Biol. 22: 585–590.
OberlanderH, LeachCE, LankaS, & WillisJH (1987) Ecdysteroid action on moth epithelial tissues and cell lines. Arch. Insect Biochem. Physiol. 5: 81–89.
OddouP, HartmannH, RadeckeF & GeiserM (1993) Immunologically unrelated Heliothis sp. and Spodoptera sp. midgut membrane-proteins bind Bacillus thuringiensis CryIA(b) δ-endotoxin. FEBS 212: 145–150.
OkaiY (1985) Insect fibroblast-like cells release inhibitory peptides of lymphocyte DNA synthesis into serum-free culture medium. Insect Biochem. 15: 483–487.
PannabeckerTL, AndrewsF & BeyenbachKW (1992). A quantitative analysis of the osmolytes in the hemolymph of the larval gypsy moth, Lymantria dispar. J. Insect Physiol. 38: 823–830.
PorcheronP (1991) Insect tissue culture systems: models for study of hormonal control of development. In Vitro Cell. Dev. Biol. 27A: 479–482.
QuiotJM, ParadisS & LouisC (1985) Establishment of a cell line (SPC G1 13) from dorsal vessels of Gromphadorhina laevigata, saussure, and zentner (Dictyoptera: Blattidae). In Vitro Cell. Develop. Biol. 21: 603–605.
RiddifordLM, KiguchiK, RoselandCR, ChenAC & WolfgangWJ (1980) Cuticle formation and sclerotization in vitro by the epidermis of the tobacco hornworm, Manduca sexta. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 103–117) Elsevier/North-Holland Biomedical Press, New York.
RochfordR, DoughertyEM & LynnDE (1984) Establishment of a cell line from embryos of the cabbage looper, Trichoplusia ni. In Vitro 20: 823–825.
Sadrud-Din S, Hakim RS & Loeb M (1992) Establishment of a midgut cell culture from Lepidoptera (abstract). In Vitro 28: 40A(I-1019).
Stanley-SamuelsonDW (1994) Assessing the significance of prostaglandins and other eicosanoids in insect physiology. J. Insect Physiol. 40: 3–11.
SangJH (1980) Drosophila cell lines. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 3–12) Elsevier/North-Holland Biomedical Press, New York.
SatoT (1989) Establishment of eight cell lines from neonate larvae of tortricids (Lepidoptera) and their several characteristics including susceptibility to insect viruses. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 2 (pp. 187–198) CRC Press Inc, Florida.
SoderhallK & SmithVJ (1983) Separation of the haemocyte populations of Carcinus maenas and other marine decapods, prophenoloxidase distribution. Dev. comp. Immunol. 7: 229–239.
SohiSS (1968) In vitro cultivation of Choristoneura fumiferana (Clemins) (Lepidopteran: Tortricidae) tissues. Can. J. Zool. 46: 11–13.
SohiSS (1971) In vitro cultivation of haemocytes of Malacosoma disstria Hubner (Lepidoptera: Lasiocampidae). Can. J. Zool. 49: 1355–1358.
SohiSS (1973) In vitro culture of larval tissues of Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae). In: J.Rehacek, BlaskovicD & HinkWF (eds), Proceedings Third International Colloquim on Invertebrate Tissue Culture (pp. 75–89) Publishing House of the Slovak Academy of Sciences, Bratislava.
SohiSS (1980) The effect of pH and osmotic pressure on the growth and survival of three Lepidopteran cell lines. In: KurstakE, MaramoroschK & DubendorferA (eds) Invertebrate Systems in vitro (pp. 35–43) Elsevier/North-Holland Biomedical Press, New York.
SteelJE (1993) Serotonin: a putative regulator of glycogen metabolism in the CNS of the cockroach, Periplaneta americana. In: BorkovecAB & LoebMJ (eds) Insect Neurochemistry and Neurophysiology (pp. 141–144) CRC Press, Ann Arbor.
StipanovicRD, ElissaldeMH, AltmanDW & NormanJO (1990) Cell culture bioassay to evaluate allelochemical toxicity to Heliothis virescens (Lepidoptera: Noctuidae). J. Econ. Entomol. 83: 737–741.
TakagiM, OkumuraH, OkadaT, KobayashiN, KiyotaT & UedaK (1994) An oxygen supply strategy for the large-scale production of tissue plasminogen activator by microcarrier cell culture. J. Ferment. Bioeng. 77: 301–306.
UiK, UedaR & MiyakeT (1989) In vitro culture of cells from dissociated imaginal disks of Drosophila melanogaster. In: MitsuhashiJ (ed) Invertebrate Cell System Applications. Vol. 2 (pp. 213–221) CRC Press Inc, Florida.
VaughnJL, GoodwinRH, TompkinsJ, & McCawleyP (1977) The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13: 213–217.
VaughnJL (1985) Insect tissue culture: techniques and development. Techniques in the Life Sciences: Setting Up and Maintenance of Tissue and Cell Cultures C108: 1–35.
WangM-Y & BentleyWE (1994) Continuous insect cell (Sf-9) culture with aeration through sparging. Appli. Microbiol. Biotechnol. 41: 317–323.
WardGB, KellyTJ, WoodsCW & MarksEP (1987) Ecdysteroid production by a continuous insect cell line. Arch. Insect Biochem. Physiol. 5: 91–98.
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Baines, D. New approaches to insect tissue culture. Cytotechnology 20, 13–22 (1996). https://doi.org/10.1007/BF00350385
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DOI: https://doi.org/10.1007/BF00350385