Abstract
A study of the seasonal dynamics of cell proliferation in the digestive tract epithelia of the mussel Crenomytilus grayanus (Bivalvia) collected in the Sea of Japan between 1971 and 1984 revealed two opposite states so far unknown for marine molluscan tissues: proliferative resting in winter and a following outburst of cell division in spring. In winter and early spring (at water temperatures below or near 0°C), mitotic figures in the lining epithelia of labial palps and intestine strongly reduced in number and later almost disappeared (February-March); DNA-synthesizing cells in these epithelia on thymidine radioautographs became sparse and only a restricted number of new cells entered the cell cycle. A proliferation block was relieved at the beginning of April (at a temperature of 2 to 4°C): an initiation of a many-fold increase in the mitotic index and some increase in the 3H-T-labelled nuclei index and their advance to the maximum in late April to May (up to more than 0.2 and 5 to 6%, accordingly) were found. In late spring (at temperatures of 5 to 10°C), a striking rise in the number of cells entering the cell cycle was observed with a probable acceleration of the cycle proper. Both seasonal states of cell proliferation — a deep depression and outburst, established in the winter-spring period are interpreted as essential events in the life cycle of the mussel studied.
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Adelstein, S. J., Lyman, C. P., O'Brien, R. S. (1967). Cell proliferation kinetics in the tongue and intenstinal epithelia of hibernating dormice (Glis glis). In: Fisher, K. C., Dawe, A. R., Lyman, C. P., Schönbaum, E., South, F. E., Jr. (eds.) Mammalian hibernation III. Oliver and Boyd, Edinburg, p. 398–408
Eliseikina, M. G. (1992). Seasonal changes in the digestive epithelium of Cucumaria japonica. Biol. Morya, Vladivostok 1/2: 69–76 (in Russian)
Epifanova, O. I. (1977). Mechanisms underlying the differential sensitivity of proliferating and resting cells to external factors. Int. Rev. Cytol. (Suppl 5): 303–335
Epifanova, O. I., Terskyh, B. B., Polunovsky, B. A. (1983). Resting cells. “Nauka”, Moscow (in Russian)
Frolova, L. T. (1979). Seasonal changes in the intestinal epithelium of Crenomytilus grayanus. In: Scarlato, O. A. (ed.) Commerical bivalve mollusks-mussels and their role in the ecosystem. Zoological Institute of Acad. Nauk USSR, Leningrad, p. 122–124 (in Russian)
Frolova, L. T. (1986). Cell reproduction in the epithelium of labial palps and esophagus of Crenomytilus grayanus. Soviet J. mar. Biol. 12: 166–173
Frolova, L. T. (1989). Morphology and proliferation in epithelia of the digestive system organs of Crenomytilus grayanus. Abstract to author's dissertation, candidate biol. sci., Vladivostok (in Russian)
Frolova, L. T., Leibson, N. L. (1991). Seasonal dynamics of mitotic activity in the intestinal epithelium in Crenomytilus grayanus. In: Leibson, N. L. (ed.) Studies of the structure, cell reproduction and regeneration of tissues with special emphasis on the digestive tract of molluscs and echinoderms: collected papers. Deposited manuscript (DEP) VINITI 26. 08. 91 N 3539-B 91, Vladivostok, p. 84–101 (in Russian)
Hoffman, R. A. (1968). The hibernator as a tool in biological research. Federation Proc. 27: 999–1007
Jaroslow, B. N., Fry, R., Suhrbier, K., Sallese, A. (1976). Radiosensitivity of ileum crypt cells in hibernating, arousing and awake ground squirrels (Citellus tridecem lineatus). Radiat. Res. 66: 566–575
Kharazova, A. D., Nechaeva, N. V. (1988). Protein synthesis in marine invertebrate cells: adaptive significance circumhous periodicity. Zh. obshch. Biol. 49: 365–375 (in Russian)
Kolaeva, S. (ed.) (1987). Mechanisms of hibernation: collected papers. Puschino Publishing House, Puschino, Russia (in Russian)
Kolaeva, S.G., Kramarova, L. I., Ilyasova, E. N., Ilyasov, F. E. (1980). The kinetics and metabolism of the cells of hibernating animals during hibernation. Int. Rev. Cytol. 66: 147–170
Kruman, I. I., Kolaeva, S. G. (1987). Peculiarities of renewal of the small intestinal epithelium in ground squirrels. In: Kolaeva, S. (ed.) Mechanisms of hibernation: collected papers. Pushino Publishing House, Puschino, Russia, p. 190–195 (in Russian)
Kruman, I. I., Kolaeva, S. G., Ilyasova, E. N., Zubrikhina, G. N., Khachko, V. N., Petrova, A. S. (1986). Sesonal variations of DNA synthesis in intestinal epithelial cells of hibernating animals-1. DNA synthesis in intestinal epithelial cells of ground squirrel (Citellus undulatus) during deep hibernation. Comp. Biochem. Physiol. 83B: 173–177
Leibson, N. L. (1982). An old way of regeneration of the intestinal epithelium during seasonal rearrangement of the intestine in the holothurian Stichopus japonicus (the possible existence of an “external cambium”). Archs Anat. Histol. Embryol. 82: 74–80 (in Russian)
Leibson, N. L. (1986). Organization of the intestinal epithelium with diffuse cell reproduction as an evolutionary stage in coelomate animals. Soviet. J. mar. Biol. 12: 261–273
Leibson, N. L. (1989). Cell reproduction in the epithelia of digestive system organs in molluscs and echinoderms. Zh. obshch. Biol. 1: 799–807 (in Russian)
Leibson, N. L., Frolova, L. T. (1975) Localization of cell division in the intestinal epithelium of marine animals. 1. Bivalve mollusc Crenomytilus grayanus. Soviet. J. mar. Biol. 1: 316–324
Leibson, N. L., Frolova, L. T. (in press). Winter-spring resting and outburst of cell reproduction in the digestive system organs of bivalve molluscs. In: Authors' abstracts of the proceedings of the 9th conference on the study of mollusc. Zoological Institute of the Russian Academy of Sciences, St. Petersburg (in Russian)
Leibson, N. L., Movchan, O. T. (1975). Cambial zones in gills of bivalvia. Mar. Biol. 31: 175–180
Sadykhova, I. A., Teis, R. V., Naidin, Yu. D. (1971). Determination of seasonal temperatures of the growth rate of shells of bivalves by the isotopic state of oxygen in skeletal carbonates. In: Licharev, I. M. (ed.) Molluscs: trends, methods and some results of their investigation. “Nauka”, Leningrad, p. 33–34 (in Russian)
Selin, N. I. (1985). The influence of environment on the formation of communities and growth of Crenomytilus grayanus. Abstract of author's dissertation, candidate biol. sci., Vladivostok (in Russian)
Suomalainen, P., Oja, H. (1967). Studies on the physiology of the hibernating hedgehod. 5. The mitotic activity in relation to seasonal and hibernation cycles. Commentat. biol. Soc. Sci. Fenn. 30: 1–6
Tokmakova, N. P., Zhad'ko, E. A., Ratakhina, L. V. (1989). Seasonal changes in mitotic activity in the epithelium of the alimentary canal of ascidians. Soviet J. mar. Biol. 1: 16–21
Usheva, L. N. (1988). Morphology and proliferation in epithelia of the digestive system organs of Mizuhopecten yessoensis. Abstract of author's dissertation, candidate biol. sci., Vladivostok (in Russian)
Usheva, L. N. (1991). Cell reproduction in the digestive tract epithelia in Mizuhopecten yessoensis. In: Leibson, N. L. (ed.) Studies of the structure, cell reproduction and regeneration of tissues with special emphasis on the digestive tract of molluscs and echinoderms: collected papers. Deposited manuscript (DEP) VINITI 26. 08. 91 n 3539-B 91, Vladisvostok p. 61–71 (in Russian)
Vinogradova, M. S. (1982). Annual cycle of changes in mitotic activity of the gastric mucosa epithelium in the hibernating rodent. Bull. exp. Biol. Med. (USSR) 43: 91–93 (in Russian)
Vorobjev, B. A. (1978). Somatic poliploidiya in the stomach epithelium of sea stars. Abstract of author's dissertation, candidate biol. sci., Moscow (in Russian)
Vorobjev, B. A. (1982). The morphology of mitoses and seasonal changes in proliferation of the starfish Asterias amurensis stomach epithelium. Citologija. 24: 580–585 (in Russian)
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Communicated by M. Vinogradov, Moscow
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Leibson, N.L., Frolova, L.T. Winter-spring essential reorganization of cell proliferation in the digestive tract epithelia in the mussel Crenomytilus grayanus . Marine Biology 118, 471–477 (1994). https://doi.org/10.1007/BF00350304
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DOI: https://doi.org/10.1007/BF00350304