Advertisement

Marine Biology

, Volume 112, Issue 1, pp 75–80 | Cite as

Tissue and sub-cellular distribution of Fe, Cu, Zn and 210Po in the abalone Haliotis rubra

  • R. V. Hyne
  • J. D. Smith
  • G. Ellender
Article

Abstract

The tissue and sub-cellular distribution of Fe, Cu, Zn and the naturally occurring radionuclide polonium-210 was determined in the gastropod mollusc Haliotis rubra collected from Western Port Bay, Australia, between March and July 1988. The highest concentrations of the metals, with the exception of Cu, were found in the digestive gland. Copper was more uniformly distributed, with tissues that are more vasculated having higher concentrations. Ultrastructural examination of the digestive gland, gill and kidney showed dense membrane-bound granules within the cytoplasm. Elemental analysis of the granules by electron probe x-ray microanalysis indicated that the granules in the digestive gland and gill contained high concentrations of iron, with small amounts of copper and zinc. In contrast, the metal-containing granules in the kidney were predominantly composed of iron, copper and phosphorus, with variable contributions of sodium potassium, and calcium. Homogenisation and fractionation of the digestive gland by differential centrifugation confirmed that approximately 80, 10, 90 and 50% of the total homogenate Fe, Cu, Zn and 210Po, respectively, sedimented at 1200xg. In the haemolymph, all the elements studied were associated with the soluble high molecular weight component of the serum, not with the amoebocytes. 210Po was present in the mucus-secreting hypobranchial glands at about half the concentration found in gill tissue.

Keywords

Radionuclide Digestive Gland Gill Tissue Variable Contribution Sodium Potassium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. Aisen, P., Listowsky, I. (1980). Iron transport and storage proteins. A. Rev. Biochem. 49: 357–393Google Scholar
  2. Al-Mohanna, S. Y., Nott, J. A. (1989). Functional cytology of the hepatopancreas of Penaeus semisulcatus (Crustacea: Decapoda) during the moult cycle. Mar. Biol. 101: 535–544Google Scholar
  3. Bacon, M. P., Spencer, D. W., Brewer, P. G. (1976). 210Pb/226Ra 210Po/210Pb disequilibria in seawater and suspended particulate material. Earth planet Sci. Lett. 32: 277–296Google Scholar
  4. Bagnall, K. W. (1973). The chemistry of selenium, tellurium and polonium. In: Schmidt, M., Siebert, W., Bagnall, K. W. (eds.) The chemistry of sulphur, selenium, tellurium and polonium. Pergamon Press, Oxford, p. 935–1008Google Scholar
  5. Bryan, G. W., Hummerstone, L. G. (1973) Brown seaweed as an indicator of heavy metals in estuaries in south-west England. J. mar. biol. Ass. U.K. 53: 705–720Google Scholar
  6. Bryan, G. W., Potts, G. W., Forster, G. R. (1977) Heavy metals in the gastropod mollusc Haliotis tuberculosa. J. mar. biol. Ass. U.K. 57: 379–390Google Scholar
  7. Bullough, W. S. (1958). Practical invertebrate anatomy. Macmillan Press, LondonGoogle Scholar
  8. Cherry, R. D., Dowdle, E. B. D., Todd, G. (1979). Intracellular sites of natural 210Po in the lobster hepatopancreas. S. Afr. J. Sci. 75: p. 39Google Scholar
  9. Cherry, R. D., Heyraud, M., Higgio, J. J. W. (1983). Polonium-210: its relative enrichment in the hepatopancreas of marine invertebrates. Mar Ecol. Prog. Ser. 13: 229–236Google Scholar
  10. Crofts, D. R. (1929). Haliotis. L. M. B. C. Mem. typ. Br. mar. Pl. Anim. 29: 1–174Google Scholar
  11. Dales, R. P. (ed) (1981). Practical invertebrate zoology. Blackwell Scientific Publications, LondonGoogle Scholar
  12. De Meio, R. H., Yu-Chem Lin, Narasimhulu, S. (1967). Some aspects of the biosynthesis of mactin. Comp. Biochem. Physiol. 20: 581–591Google Scholar
  13. Ellerton, H. D., Lankovsky, T. (1983). Structure of the haemocyanin from pau (abalone) Haliotis iris. Life Chem. Rep. (Suppl.) 1: 129–132Google Scholar
  14. Fisher, N. S., Smith, J. D. (1987). Molecular association of Cu, Zn, Cd and 210Po in the digestive gland of the squid Nototodarus gouldi. Mar. Biol 95: 87–91Google Scholar
  15. Fisher, N. S., Burns, K. A., Cherry, R. D., Heyraud, M. (1983). Accumulation and cellular distribution of 241Am, 210Po and 210Pb in two marine algae. Mar. Ecol. Prog. Ser. 11: 233–237Google Scholar
  16. Folsom T. R., Wong, K. M., Hodge, V. F. (1974). Some extreme accumulations of natural polonium radioactivity observed in certain occanic organisms. In: Adams, J. A. S., Lowder, W. M., Gesell, T. F. (eds) The natural radiation environment. II.Texas University Press, Houston, Texas, p. 863–882Google Scholar
  17. Fox, D. L. (1966). Pigmentation in molluscs. In: Wilbur, K. M., Yonge, C. M. (eds.) Physiology of Mollusca. Vol. 2. Academic Press, New York, p. 249–274Google Scholar
  18. Guary, J. C., Negrel, R. (1981). Calcium phosphate granules: a trap for transuranics and iron in crab hepatopancreas. Comp. Biochem. Physiol. 68A: 423–427Google Scholar
  19. Hamilton, T. F., Smith, J. D. (1986). Improved alpha energy resolution for the determination of polonium isotopes by alpha-spectrometry. Appl. Radiat. Isotopes 37: 628–630Google Scholar
  20. Heyraud, M., Cherry, R. D., Dowdle, E. B. (1987). The subcellular localisation of natural 210Po in the hepatopancreas of the rock lobster (Jasus lalandii) J. envirl Radioact. 5: 249–260Google Scholar
  21. Heyraud, M., Domanski, P., Cherry, R. D., Fasham, M. J. R. (1988). Natural tracers in dietary studies: data for 210Po and 210Pb in decapod shrimp and other pelagic organisms in the Northeast Atlantic Ocean. Mar. Biol. 97: 507–529Google Scholar
  22. Hunt, S., Jevons, F. R. (1966). The hypobranchial mucin of the whelk Buccinum undatum L. Biochem. J. 98: 522–529Google Scholar
  23. Kawai, K. (1961). Comparative biochemical studies on cytochromes and related substances of invertebrates. II. Cytochrome-like haemoproteins in the gut fluids of mulluscs. Biochim. biophys. Acta 52: 241–247Google Scholar
  24. Pilson, M. E. (1965). Variation of hemocyanin concentration in the blood of four species of Haliotis. Biol. Bull. mar. biol. Lab., Woods Hole 128: 459–472Google Scholar
  25. Reynold, E. S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Cell Biol. 17: 209–212Google Scholar
  26. Simkiss, K., Mason, A. Z. (1983). Metal ions: metabolic and toxic effects. In: Hochachka, O. W. (ed.) The Mollusca. Vol 2. Academic Press, New York, p. 101–164Google Scholar
  27. Smith, J. D., Hamilton, T. F. (1984). Improved technique for recovery and measurement of polonium-210 from environmental materials. Analytica chim. Acta 160: 69–77Google Scholar
  28. Smith, J. D., Plues, L., Heyraud, M., Cherry, R. D. (1984). Concentrations of the elements Ag, Al Ca, Cd, Cu, Fe, Mg, Mn, Pb and Zn and the radionuclides 210Pb and 210Po in the digestive gland of the squid Nototodarus gouldii. Mar. envirl Res. 13: 55–68Google Scholar
  29. Spurr, A. R. (1969). A low-viscosity epoxy-resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26: 31–43Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • R. V. Hyne
    • 1
  • J. D. Smith
    • 1
  • G. Ellender
    • 2
  1. 1.Marine Chemistry Laboratory, School of ChemistryUniversity of MelbourneParkvilleAustralia
  2. 2.School of Dental ScienceUniversity of MelbourneParkvilleAustralia

Personalised recommendations