Abstract
Marine bdellovibrios have not previously been reported from the southern hemisphere, and knowledge of their occurrence in marine ecosystems is rudimentary. This study examined quantitative and qualitative aspects of bdellovibrios parasitic to the bacterium Vibrio alginolyticus at each of three representative tropical marine habitats of the Great Barrier Reef. Bdellovibrios were found in the water column throughout a 12 mo period from May 1992 at a sandy beach, a mangrove and a fringing coral reef. Their abundance was correlated with water temperature (P<0.001) and was highest in summer, lowest in winter and intermediate in spring and autumn. Over the sampling period, bdellovibrios were most abundant at the mangrove habitat (36.6 ml-1) and least abundant at the reef (9.5 ml-1), but there was substantial variability in numbers at all habitats among seasons and months of the year. On some occasions no bdellovibrios were found in replicate samples from the beach and reef habitats, while on others the maximum detectable by the method used (180 ml-1) was sometimes found at the beach and mangrove habitats. Bdellovibrios within each habitat were uniformly distributed among sampling sites (P>0.05). They were more abundant in sub-surface than bottom waters in summer, but the reverse occurred in winter. Midwater samples usually had least bdellovibrios. Bdellovibrio numbers were significantly correlated with those of potential host bacteria—colony-forming bacteria at all habitats and total bacteria at the beach and reef habitats. Strain characteristics, primarily based on host range, indicated qualitative differences in bdellovibrio populations among habitats. Pseudomonas atlantica, P. aeruginosa, P. marina, Cytophaga marinoflava, Vibrio gazogenes, V. mimicus and a Spirillum-like bacterium were not parasitised by bdellovibrios from any habitat. Of the other 25 Vibrio spp. tested, most were parasitised by the majority of bdellovibrio strains from each habitat. Strain differences were principally with respect to parasitism of non-Vibrio bacteria. All strains required Na+ and grew at 35°C, but some failed to grow at 15°C.
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References
Atlas RM, Bartha R (1981) Microbial ecology. Fundamentals and applications. Addison-Wesley, London
Azam F, Fenchel T, Field JG, Gray JS, Meyer-Reil LA, Thingstad F (1983) The ecological role of water-column microbes in the sea. Mar Ecol Prog Ser 10:257–263
Collins CH, Lyne PM, Grange JM (eds) (1989) Collins and Lyne's microbiological methods. Butterworths, London
Hobbie JE, Daley RJ, Jasper S (1977) Use of Nuclepore filters for counting bacteria in the salt marsh environment. Appl envirol Microbiol 33:1225–1228
Kirchman DL, Peterson B, Juers D (1984) Bacterial growth and tidal variation in bacterial abundance in the Great Sippewissett salt marsh. Mar Ecol Prog Ser 19:247–259
Marbach A, Shilo M (1978) Dependence of marine bdellovibrio on potassium, calcium, and magnesium ions. Appl envirol Microbiol 36:169–177
Marbach A, Varon M, Shilo M (1976) Properties of marine bdellovibrios. Microb Ecol 2:284–295
Reichelt JL, Baumann P (1973) Taxonomy of the marine luminous bacteria. Arch Mikrobiol 94:283–330
Sanchez Amat A, Torrella F (1989) Isolation and characterisation of marine and salt pond halophylic bdellovibrios. Can J Microbiol 35:771–778
Schoeffield AJ, Williams HN (1990) Efficiencies of recovery of bdellovibrios from brackish-water environments by using various bacterial species as prey. Appl envirl Microbiol 59:230–236
Shilo M (1966) Predatory bacteria. Science, NY 2:33–37
Shilo M (1969) Morphological and physiological aspects of the interaction of bdellovibrio with its host bacteria. Curr Topics Microbiol Immunol 50:174–204
Starr MP, Baigent NL (1966) Parasitic interaction of Bdellovibrio bacteriovorus with other bacteria. J Bact 91:2006–2017
Starr MP, Huang JCC (1972) Physiology of the bdellovibrios. Adv microb Physiol 8:215–261
Starr MP, Stolp H (1976) Bdellovibrio methodology. In: Norris JR (ed) Methods in microbiology. Vol 9. Academic Press, London, p 217–244
Stolp H (1981) The genus Bdellovibrio. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG (eds) The prokaryotes, a handbook of habitats, isolation and identification of bacteria. Vol 1. Springer-Verlag, Berlin, p 618–629
Taylor VT, Baumann P, Reichelt JL, Allen RD (1974) Isolation, enumeration and host range of marine bdellovibrios. Archs Microbiol 98:101–114
Underwood AJ (1981) Techniques of analysis of variance in experimental marine biology and ecology. Oceanogr mar Biol A Rev 19:513–605
Varon M, Shilo M (1980) Ecology of aquatic bdellovibrios. In: Droop MR, Jannasch HW (eds) Advances in aquatic microbiology, Vol 2. Academic Press, New York, p 1–48
Williams HN (1987) The recovery of high numbers of bdellovibrios from the surface water microlayer. Can J Microbiol 33:572–575
Williams HN (1988) A study of the occurrence and distribution of bdellovibrios in estuarine sediment over an annual cycle. Microb Ecol 15:9–20
Williams HN, Falkler WA, Jr (1984) Distribution of bdellovibrios in water column of an estuary. Can J Microbiol 30:971–974
Williams HN, Falkler WA, Jr, Shay DE (1981) Seasonal distribution of bdellovibrios at the mouth of the Patuxent river in the Chesapeake Bay. Can J Microbiol 28:111–116
Zar JH (1984) Biostatistical analysis. Prentice-Hall, Englewood Cliffs, New Jersey
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Communicated by G. F. Humphrey, Sydney
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Sutton, D.C., Besant, P.J. Ecology and characteristics of bdellovibrios from three tropical marine habitats. Marine Biology 119, 313–320 (1994). https://doi.org/10.1007/BF00349571
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DOI: https://doi.org/10.1007/BF00349571