Marine Biology

, Volume 114, Issue 2, pp 317–325 | Cite as

DNA fingerprints of a gorgonian coral: a method for detecting clonal structure in a vegetative species

  • Mary Alice Coffroth
  • Howard R. Lasker
  • Margaret E. Diamond
  • Jeremy A. Bruenn
  • Eldredge Bermingham


Clonal reproduction, a common life history strategy among sessile marine invertebrates, can lead to high local abundances of one to a few genotypes in a population. Analysis of the clonal structure of such populations can provide insight into the ecological and evolutionary history of the population, but requires markers that can identify individual genets. Forensic and demographic studies have demonstrated that DNA fingerprinting can provide markers that are unique for an individual genotype. We have generated DNA fingerprints for over 70 colonies of the clonal gorgonian, Plexaura A (Plexaura sp. A) collected from June 1990 through July 1991 in the San Blas Islands, Panama. DNA fingerprints within a singic individual were identical and fingerprinting resolved multiple genotypes within and among reefs. On one reef in the San Blas Islands, Panama, 59% of the colonies sampled were of one genotype and this genotype was not found on any other sampled reefs. A previous study using tissue grafts identified 13 putative clones on these reefs, while DNA fingerprints of the same colonies differentiated 17 genotypes. The present study demonstrates the utility of DNA fingerprinting for distinguishing clones and for identifying clonal structure of marine invertebrate populations.


Marine Invertebrate Life History Strategy Individual Genotype Tissue Graft Local Abundance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Literature cited

  1. Ayre, D. J. (1983). The effects of asexual reproduction and intergenotypic aggression on the genotypic structure of populations of the sea anempone Actinia tenebrosa. Oecologia 57: 158–165Google Scholar
  2. Ayre, D. J. (1984). The effect of sexual and asexual reproduction on geographic variation in the sea anemone Actinia tenebrosa. Oecologia 62: 222–229Google Scholar
  3. Ayre, D. J., Willis, B. L. (1988). Population structure in the coral Pavona cactus: clonal genotypes show little phenotypic plasticity. Mar. Biol. 99: 494–505Google Scholar
  4. Bellamy, R. J., Inglehearn, C. F., Jalili, I. K., Jeffreys, A. J., Bhattacharya, S. S. (1991). Increased band sharing in DNA fingerprints in an inbred human population. Hum. Genet. 87: 341–347Google Scholar
  5. Birkhead, T. R., Burke, T., Zann, R., Hunter, F. M., Krupa, A. P. (1990). Extra-pair paternity and intraspecific brood parasitism in wild zebra finches Taeniopygia guttata, revealed by DNA fingerprinting. Behavl Ecol. Sociobiol. 27: 315–324Google Scholar
  6. Brazeau, D. A., Lasker, H. R. (1989). The reproductive cycle and spawning in a Caribbean gorgonian. Biol. Bull. mar. Biol. Lab., Woods Hole 176: 1–7Google Scholar
  7. Burke, J., Bruford, M. W. (1987). DNA fingerprinting in birds. Nature. Lond. 327: 149–152Google Scholar
  8. Burke, T., Davies, N. B., Bruford, M. W., Hatchwell, B. J. (1989). Parental care and mating behavior of polyandrous dunnocks Prunella modularis related to paternity by DNA fingerprinting. Nature, Lond. 338: 249–251Google Scholar
  9. Carvalho, G. R., Maclean, N., Wratten, S. D., Carter, R. E., Thurston, J. P. (1991). Differentiation of aphid clones using DNA fingerprints from individual aphids. Proc. R. Soc. (Ser. B) 243: 109–114Google Scholar
  10. Curtis, A. S. G., Kerr, J., Knowlton, N. (1982). Graft rejection in sponges. Transplantation 33: 127–133Google Scholar
  11. Faulkes, C. G., Abbott, D. H., Mellor, A. L. (1990). Investigation of genetic diversity in wild colonies of naked mole-rats (Heterocephalus glaber) by DNA fingerprinting. J. Zool., Lond. 221: 87–97Google Scholar
  12. Feinberg, A. P., Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analyt. Biochem. 132: 6–13Google Scholar
  13. Gilbert, D. A., Lehman, N., O'Brien, S. J., Wayne, R. K. (1990) Genetic fingerprinting reflects population differentiation in the California Channel Island fox. Nature, Lond. 344: 764–767Google Scholar
  14. Hebert, P. D. N., Beaton, M. J., Schwartz, S. S., Stanton, D. J. (1989). Polyphyletic origins of asexuality in Daphnia pulex. I. Breeding-system variation and levels of clonal diversity. Evolution, Lawrence Kansas 43: 1004–1015Google Scholar
  15. Hebert, P. D. N., Ward, R. D., Weider, L. J. (1988). Clonal-diversity patterns and breeding-system variation in Daphnia pulex, an asexual-sexual complex. Evolution, Lawrence, Kansas, 42(1): 147–159Google Scholar
  16. Highsmith, R. C. (1982). Reproduction by fragmentation in corals. Mar. Biol. Prog. Ser. 7: 207–226Google Scholar
  17. Hoffmann, R. J. (1976). Genetics and asexual reproduction of the sea anemone Metridium senile. Biol. Bull. mar. biol. Lab., Woods Hole 151: 478–488Google Scholar
  18. Hoffmann, R. J. (1986). Variation in contributions of asexual reproduction to the genetic structure of populations of the sea anemone Metridium senile. Evolution, Lawrence, Kansas, 40(2): 357–365Google Scholar
  19. Hoffmann, R. J. (1987). Short-term stability of genetic structure in populations of the sea anemone Metridium senile. Mar. Biol 93: 499–507Google Scholar
  20. Hunter, C. L. (1985). Assessment of clonal diversity and population structure of Porites compressa (Cnidaria, Scleractinia). Proc. 5th int. coral Reef Congr. 6: 69–74 [Gabrié, C. et al. (eds.) Antenne Museum — EPHE, Moorea, French Polynesia]Google Scholar
  21. Jackson, J. B. C. (1985). Distribution and ecology of clonal and aclonal benthic invertebrates. In: Jackson, J. B. C., Buss, L. W., Cook, R. F. (eds.) Population biology and evolution of clonal organisms. Yale University Press, New Haven, Connecticut, USA, p. 297–355Google Scholar
  22. Jeffreys, A. J., Morton, D. B. (1987). DNA fingerprinting of dogs and cats. Anim. Genet. 18: 1–16Google Scholar
  23. Jeffreys, A. J., Royle, N. J., Wilson, V., Wong, Z. (1988). Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA. Nature, Lond. 322: 278–281Google Scholar
  24. Jeffreys, A. J., Wilson, V., Kelly, R., Taylor, B. A., Bulfield, G. (1987). Mouse DNA ‘fingerprints’: analysis of chromosome localisation and germ-line stability of hypervariable loci in recombinant inbred strains. Nucleic Acids Res. 15: 2823–2836Google Scholar
  25. Jeffreys, A. J., Wilson, V., Thein, S. L. (1985a). Hypervariable ‘minisatellite’ regions in human DNA. Nature Lond. 314: 67–73Google Scholar
  26. Jeffreys, A. J., Wilson, V., Thein, S. L. (1985b). Individual-specific ‘fingerprints’ of human DNA. Nature, Lond. 316: 76–79Google Scholar
  27. Johnson, M. S., Threlfall, T. J. (1987). Fissiparity and population genetics of Coscinasterias calamaria. Mar. Biol. 93: 517–525Google Scholar
  28. Kuhnlein, U., Zadworny, D., Dawe, Y., Fairfull, R. W., Gavora, J. S. (1990). Assessment of inbreeding by DNA fingerprinting: development of a calibration curve using defined strains of chicken. Genetics, Baltimore, Md 125: 161–165Google Scholar
  29. Lasker, H. R. (1984). Asexual reproduction, fragmentation, and skeletal morphology of a plexaurid gorgonian. Mar. Ecol. Prog. Ser. 19: 261–268Google Scholar
  30. Lasker, H. R. (1990). Clonal propagation and population dynamics of a gorgonian coral. Ecology 71: 1578–1589Google Scholar
  31. Lasker, H. R., Coffroth, M. A. (1985). Vegetative reproduction, clonal spread, and histocompatibility in a Caribbean gorgonian. Proc. 5th intl. coral Reef Congr. 4: 331–336. [Gabrié, C. et al. (eds.) Antenne Museum — EPHE, Moorea, French Polynesia]Google Scholar
  32. Lynch, M. (1988). Estimation of relatedness by DNA fingerprinting. Molec. Biol. Evolut. 5(5): 584–599Google Scholar
  33. Lynch, M. (1990). The similarity index and DNA fingerprinting. Molec. Biol. Evolut. 7: 478–484Google Scholar
  34. Neigel, J. E., Avise, J. C. (1983a) Clonal diversity and population structure in a reef-building coral, Acropora cervicornis: self-recognition analysis and demographic interpretation. Evolution, Lawrence, Kansas 37: 437–453Google Scholar
  35. Neigel, J. E., Avise, J. C. (1983b). Histocompatibility bioassays of population structure in marine sponges. J. Hered. 74: 134–140Google Scholar
  36. Nybom, H., Rogstad, S. H. (1990) DNA “fingerprints” detect genetic variation in Acer negundo (Aceraceae). Pl. Syst. Evol. 1734: 49–56Google Scholar
  37. Nybom, H., Schaal, B. A. (1990). DNA “fingerprints” reveal genotypic distributions in natural populations of blackberries and raspberries (Rubus, Rosaceae). Am. J. Bot. 77: 883–888Google Scholar
  38. Reeve, H. K., Westneat, D. F., Noon, W. A., Sherman, P. W., Aquadro, C. F. (1990). DNA “fingerprinting” reveals high levels of inbreeding in colonies of the eusocial naked mole-rat. Proc. natn. Acad. Sci. U.S.A. 87: 2496–2500Google Scholar
  39. Rogstad, S. H., Nybom, H., Schaal, B. A. (1991). The tetrapod DNA fingerprinting M13 repeat probe reveals genetic diversity and clonal growth in quaking aspen (Populus tremuloides Salicacceae). Pl. Syst. Evol. 175: 115–123Google Scholar
  40. Rogstad, S. H., Patton, J. C., Schaal, B. A. (1988). M13 repeat probe detects DNA minisatellite-like sequences in gymnosperms and angiosperms. Proc. natn. Acad. Sci. U.S.A. 85: 9176–9178Google Scholar
  41. Rowan, R., Powers, D. A. (1991). Molecular genetic identification of symbiotic dinoflagellates (zooxanthellae). Mar. Biol. 71: 65–73Google Scholar
  42. Ryskov, A. P., Jincharadze, A. G., Prosnyak, M. I., Ivanov, P. L., Limborska, S. A. (1988). M13 phage DNA as a universal marker for DNA fingerprinting of animals, plants and microorganisms. Fedn. eur. biochem. Soc. (FEBS) Lett. 233(2): 388–392Google Scholar
  43. Saghai-Maroof, M. A., Sollman, K. M., Jorgensen, R. A., Allard, R. W. (1984). Ribosomal DNA spacer-length polymorphisms in barley. Mendelian inheritance, chromosomal location and population dynamics. Proc. natn. Acad. Sci. U.S.A. 81: 8014–8018Google Scholar
  44. Stoddart, J. A. (1984). Genetical structure within populations of the coral Pocillopora damicornis. Mar. Biol. 81: 19–30Google Scholar
  45. Stoddart, J. A., Ayre, D. J., Willis, B., Heyward, A. J. (1985). Selfrecognition in sponges and corals? Evolution, Lawrence, Kansas 39: 461–463Google Scholar
  46. Turner, B. J., Elder, J. F., Laughlin, T. F., Davis, W. P. (1990). Genetic variation in clonal vertebrates detected by simple-sequence DNA fingerprinting. Proc. natn. Acad. Sci. U.S.A. 87: 5653–5657Google Scholar
  47. Uitterlinden, A. G., Slagboom, P. E., Johnson, T. E., Vijg, V. (1989). The Caenorhabditis elegans genome contains monomorphic minisatellites and simple sequences. Nucleic Acids Res. 17: 9527Google Scholar
  48. Vassart, G., Georges, M., Monsieur, R., Brocas, H., Lequarre, A. S., Christophe, D. (1987). A sequence in the M13 phage detects hypervariable minisatellite in human and animal DNA. Science, N.Y. 235: 683–684Google Scholar
  49. Westneat, D. F. (1990). Genetic parentage in the indigo bunting: a study using DNA fingerprinting. Behavl Ecol. Sociobiol. 27: 67–76Google Scholar
  50. Westneat, D. F., Noon, W. A., Reeve, H. K., Aquadro, C. F. (1988). Improved hybridization conditions for DNA “fingerprinting” probed with M13. Nucleic Acids Res. 16: 4161Google Scholar
  51. Wetton, J. H., Carter, R. E., Parkin, D. T., Walters, D. (1987). Demographic study of a wild house sparrow population by DNA fingerprinting. Nature, Lond. 327: 147–149Google Scholar
  52. Williams, G. C. (1975). Sex and evolution. Princeton University Press, PrincetonGoogle Scholar
  53. Willis, B. L., Ayre, D. J. (1985). Asexual reproduction and genetic determination of growth form in the coral Pavona cactus: biochemical, genetic and immunogenic evidence. Oecologia 65: 516–525Google Scholar
  54. Wulff, J. L. (1986). Variation in clone structure of fragmenting coral reef sponges. Biol. J. Linn. Soc. 27: 311–330Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Mary Alice Coffroth
    • 1
  • Howard R. Lasker
    • 1
  • Margaret E. Diamond
    • 1
  • Jeremy A. Bruenn
    • 1
  • Eldredge Bermingham
    • 2
  1. 1.Department of Biological ScienceState University of New York at BuffaloBuffaloUSA
  2. 2.Smithsonian Tropical Research InstituteBalboaRepublic of Panama

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