Marine Biology

, Volume 114, Issue 2, pp 229–233 | Cite as

Quantification of arginine requirements of juvenile marine shrimp, Penaeus monodon, using microencapsulated arginine

  • H.-Y. Chen
  • Y.-T. Leu
  • I. Roelants


Using microencapsulated L-arginine, the quantitative requirement for amino acid has been determined for the first time for a shrimp species, which can not effectively utilize crystalline amino acids. In an 8 wk feeding trial (1990), juvenile Penaeus monodon were fed casein-based purified diets containing one of six levels (13.1, 17.7, 22.3, 26.9, 31.5 and 36.1 g/kg diet) of arginine. In addition to the protein-bound arginine already present in the casein of the test diets, pure arginine was supplemented by L-arginine microencapsulated in cellulose acetate phthalate, which is easily assimilated. The arginine level required for optimal growth was determined by brokenline model analysis of weight gain data to be 25.0 g/kg diet (=54.7 g/kg protein). Examination of the hemolymph 3 h after feeding revealed that the free arginine level in the hemolymph had not increased as a result of increasing levels of the dietary arginine. There was an abrupt increase of urea in the hemolymph when the arginine requirement of the shrimp had been met.


Arginine Phthalate Cellulose Acetate Feeding Trial Test Diet 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • H.-Y. Chen
    • 1
  • Y.-T. Leu
    • 1
  • I. Roelants
    • 1
  1. 1.Institute of Marine BiologyNational Sun Yat-sen UniversityKaohsiungTaiwan, Republic of China

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