Abstract
Turbot (Scophthalmus maximus L.) were reared at 12 and 16°C until 26 d after hatching. At both temperatures, starting at the neural plate stage, somites were initially formed every 75 min. Expressed as a percentage of development time (DT, fertilisation to 90% larvae hatching) somite formation occurred relatively earlier during embryogenesis at 12°C (45% DT) than at 16°C (55% DT). At 12°C, after the 32-somite stage the rate of somite formation decreased to one every 300 min. The larvae hatched after 6 d at 12°C and 3 d at 16°C at a relatively primitive stage of development, prior to the opening of the mouth and anus, with unpigmented eyes, and a straight gut. Temperature altered the relative timing of organogenesis in the larval stages. At 12°C, the following characters appeared (in this order): swimbladder>loop in the gut (at the time of yolk exhaustion)>caudal fin. In contrast, at 16°C, the caudal fin appeared at the same time as the loop in the gut. At 16°C, spines formed on the head in the region of the otic capsule at the time the swimbladder formed and the yolk was exhausted, but were absent in 12°C larvae. At both temperatures, in 1 d-old larvae the myotomes just behind the yolk-sac contained ∼ 200 inner muscle fibres (presumptive white muscle). The initial growth of inner muscle was largely due to hypertrophy, but by 26 d at 12°C and 11 d at 16°C hyperplastic growth became important, as evidenced by a significant increase in the number of small fibres (<10 μm2). By 26 d the average number of inner muscle fibres had increased to 341 at 12°C and 988 at 16°C. New muscle fibres were added in distinct germinal zones at the dorsal and ventral apices of the myotomes. Metamorphosis was associated with a thickening of the superficial (presumptive red) muscle layer and the appearance of tonic muscle fibres.
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References
Al-Maghazachi SJ, Gibson R (1984) The development stages of larval turbot, Scophthalmus maximus (L.). J exp Biol 82: 35–51
Batty RS (1984) Development of swimming movements and musculature of larval herring (Clupea harengus). J exp Biol 110: 217–229
Batty RS, Blaxter JHS, Fretwell K (1993) Effect of temperature on escape responses of larval herring, Clupea harengus. Mar Biol 115: 523–528
Blaxter JHS (1988) Pattern and variety in development. In: Hoar WS, Randall DJ (ed) Fish physiology. Vol. XIA. Academic Press, New York, pp 1–58
Brooks S, Johnston IA (1993) Influence of development and rearing temperature on the distribution, ultrastructure and myosin subunit composition of myotomal muscle-fibre types in the plaice Pleuronectes platessa. Mar Biol 117: 501–513
Calvo J, Johnston IA (1992) Influence of rearing temperature on the distribution of muscle fibre types in the turbot Scophthalmus maximus at metamorphosis. J exp mar Biol Ecol 161: 45–55
Crockford T, Johnston IA (1993) Developmental changes in the composition of myofibrillar proteins in the swimming muscles of Atlantic herring, Clupea harengus. Mar Biol 115: 15–22
Dau F (1884) The fishes of Great Britain and Ireland. Vol. II. Williams & Norgate, London
Edwards AL (1962) Statistical methods for the behavioural sciences. Holt, Rinehart & Winston, New York
Fahy WE (1981) The influence of temperature change in the number of dorsal fin rays developing in Fundulus majalis (Walbaum). Rapp P-v Réum Cons perm int Explor Mer 178: p. 568
Fonds M, Rosenthal H, Alderdice DF (1974) Influence of temperature and salinity on embryonic development, larval growth and number of vertebrae of the garfish, Belone belone. In: JHS Blaxter (ed) The early life history of fish. Springer-Verlag, Berlin, New York, pp 509–525
Fukuhara O (1990) Effects of temperature on yolk utilization, initial growth, and behaviour of unfed marine fish-larvae. Mar Biol 106: 169–174
Hannerman EH (199) Diisopropylflurophosphate inhibits acetylcholinesterase activity and disrupts somitogenesis in the zebra fish. J exp Zool 262: 41–53
Hannerman EH, Westerfield M (1989) Early expression of acetylcholinesterase activity in functionally distinct neurons of the zebrafish. J comp Neurol 333: 289–300
Holt EWL (1892) Rhombus maximus. Linn. (the turbot). J mar biol Ass UK 36: 539–552
Hovencamp F (1991) On the growth of larval plaice in the North Sea. PhD thesis. Rijksuniversiteit, Groningen
Johnston IA (1993) Temperature influences muscle differentiation and relative timing of organogenesis in herring (Clupea harengus) larvae. Mar Biol 116: 363–379
Johnston IA, Vieira VLA, Abercromby M (1995) Temperature and myogenesis in embryos of the Atlantic herring (Clupea harengus). J exp Biol 198: 1389–1403
Jones A (1972) Studies on egg development and larval rearing of turbot, Scophthalmus maximus L. and brill, Scophthalmus rhombus L., in the laboratory. J mar biol Ass UK 52: 965–986
Jordan DS (1892) Relations of temperature to vertebrae among fishes. Proc US natn Mus 14: 107–120
Karnovsky MJ, Roots L (1964) A direct colouring, thiocholine method for cholinesterases. J Histochem Cytochem 12: 219–221
Lasker R (1981) Marine fish larvae: morphology, ecology and relation to fisheries. University of Washington Press, Washington
Lindsey CC (1975) Pleomerism, the widespread tendency for vertebral number to be correlated with maximum body depth. J Fish Res Bd Can 32: 2453–2569
Lindsey CC (1988) Factors controlling meristic variation. In: Hoar WS, Randall DJ (eds) Fish physiology. Vol. IIB. Academic Press, New York, pp 197–262
Riley JD, Symonds DJ, Woolner (1981) On the factors influencing the distribution of O-group demersal fish in coastal waters. Rapp P-v Réun Cons perm int Explor Mer 178: 223–228
Russell FS (1976) Eggs and planktonic stages of British marine fish. Academic Press, London
Ryland JS (1963) The swimming speeds of plaice larvae. J exp Biol 40: 285–299
Ryland JS (1966) Observations on the development of larvae of the plaice, Pleuronectes platessa L., in aquaria. J Cons perm int Explor Mer 30: 177–195
Ryland JS, Nichols JH, Sykes AM (1975) Effects of temperature on the embryonie development of plaice, Pleuronectes platessa L. (Teleostei). J exp mar biol Ecol 18: 121–137
Shelbourne JE (1957) The feeding and condition of plaice larvae in good and bad plankton patches. J mar biol Ass UK 36: 539–552
Stickland NC, White RN, Mescall PE, Crook AE, Thorpe JE (1988) The effect of temperature on myogenesis in embryonic development of the Atlantic salmon (Salmo salar, L.). Anat Embryol 178: 253–257
Swain DF (1992) The functional basis of natural selection for vertebral traits of larvae in the stickleback Gasterosteus aculeatus. Evolution 46: 987–997
Taning AV (1952) Experimental study of meristic characters in fishes. Biol Rev 27: 169–193
Vieira VLA, Johnston IA (1992) Influence of temperature on musclefibre development in larvae of the herring Clupea harengus. Mar Biol 112: 333–341
Weatherley AH, Gill HS (1985). Dynamics of increase in musclefibres in fishes in relation to size and growth. Experientia 41: 353–354
Weatherley AH, Gill HS, Rogers SC (1980) The relationship between mosaic muscle fibres and size in rainbow trout (Salmo gairdneri). J Fish Biol 17: 603–610
Wheeler A (1978) Key to the fishes of northern Europe. Frederick Warne & Co. Ltd, London
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Communicated by J. Mauchline, Oban
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Gibson, S., Johnston, I.A. Temperature and development in larvae of the turbot Scophthalmus maximus . Marine Biology 124, 17–25 (1995). https://doi.org/10.1007/BF00349142
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DOI: https://doi.org/10.1007/BF00349142