Abstract
Zoospores, gametophytes, young sporophytes and discs cut from mature sporophytes of Laminaria digitata, L. hyperborea and L. saccharina were exposed in the laboratory to UV-radiation, with a spectral composition and irradiance similar to natural sunlight, for periods ranging from 15 min to 8 d, and were then returned to white light. Germination of zoospores and the growth of gametophytes were reduced after exposures to UV longer than 1 h, whereas UV had little effect on the growth of young or mature sporophytes unless exposure continued for more than 48 h. The variable fluorescence (F v:Fm) of all stages was strongly reduced immediately after short exposures to UV, but recovered almost completely within 24 h. However, exposure of gametophytes to UV for >4 h resulted in little or no recovery of F v:Fm, whereas >16 h of UV were required to produce this result in young sporophytes, and >48 h in mature sporophytes. Thus, sensitivity to UV-radiation decreased from gametophytes to sporophytes, and with increasing age of sporophytes, but, in gametophytes, growth appeared to be a more sensitive indicator of UV-damage than F v:Fm after 24 h recovery. The responses to UV of the zoospores and gametophytes of all three species were similar, but both growth and fluorescence measurements suggested that the sporophytes of L. saccharina were more sensitive to UV than those of the other two species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Björkman O, Demmig B (1987) Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta 170: 489–504
Blumthaler M, Ambach W (1990) Indication of increasing solar ultraviolet-B radiation flux in alpine regions. Science, NY 248: 206–208
Bose S, Herbert SK, Fork DC (1988) Fluorescence characteristics of photoinhibition and recovery in a sun and shade species of the red algal genus Porphyra. Pl Physiol 86: 946–950
Büchel C, Wilhelm C (1993) In vivo analysis of slow fluorescence induction kinetics in algae: progress, problems and perspectives. Photochem Photobiol 58: 137–148
Farman JC, Gardiner BG, Shanklin JD (1985) Large losses of total ozone in Antarctica reveal seasonal ClOx/NOx interaction. Nature, Lond 314: 207–210
Han T, Kain JM (1996) Effect of photon irradiance and photoperiod on young sporophytes of four species of the Laminariales. Eur J Phycol (in press)
Hanelt D, Huppertz K, Nultsch W (1992) Photoinhibition of photosynthesis and its recovery in red algae. Botanica Acta (Ber dt bot Ges) 105: 278–284
Hanelt D, Huppertz K, Nultsch W (1993) Daily course of photosynthesis and photoinhibition in marine macroalgae investigated in the laboratory and field. Mar Ecol Prog Ser 97: 31–37
Hanelt D, Li J, Nultsch W (1994) Tidal dependence of photoinhibition of photosynthesis in marine macrophytes of the South China Sea. Botanica Acta (Ber dt bot Ges) 107: 66–72
Hanelt D, Nultsch W (1994) Field studies of photoinhibition show non-correlations between oxygen and fluorescence measurements in Arctic red alga Palmaria palmata. J Pl Physiol 145: 31–38
Hanelt D, Uhrmacher S, Nultsch W (1995) The effect of photoinhibition on photosynthetic oxygen production in the brown alga Dictyota dichotoma. Botanica Acta (Ber dt bot Ges) 108: 99–105
Henley WJ (1992) Growth and photosynthesis of Ulva rotundata (Chlorophyta) as a function of temperature and square wave irradiance in indoor culture. J Phycol 28:625–634
Huppertz K, Hanelt D, Nultsch W (1990) Photoinhibition of photosynthesis in the marine brown alga Fucus serratus as studied in field experiments. Mar Ecol Prog Ser 66: 175–182
Grobe CW, Murphy TM (1994) Inhibition of growth of Ulva expansa (Chlorophyta) by ultraviolet-B radiation. J Phycol 30: 783–790
Kain JM (1966) The role of light in the ecology of Laminaria hyperborea. In: Bainbridge R, Evans GC, Rackham O (eds) Light as an ecological factor. Blackwell Scientific, Oxford, pp 319–334
Kerr JB, McElroy CT (1993) Evidence for large upward trends of ultraviolet-B radiation linked to ozone depletion. Science, NY 262: 1032–1034
Larkum AWD, Wood WF (1993) The effect of UV-B radiation on photosynthesis and respiration of phytoplankton, benthic macroalgae and seagrasses. Photosynthesis Res 36: 17–23
Lüning K (1970) Tauchuntersuchungen zur Vertikalverteilung der sublittoralen Helgoländer Algenvegetation. Helgoländer wiss Meeresunters 21: 271–291
Lüning K (1980) Critical levels of light and temperature regulating the gametogenesis of three Laminaria species (Phaeophyceae). J Phycol 16: 1–15
Lüning K (1992) Day and night kinetics of growth rate in green, brown and red seaweeds. J Phycol 28: 794–803
Mann KH (1973) Seaweeds: their productivity and strategy for growth. Science, NY 182: 975–981
Nishio JN, Sun J, Vogelmann TC (1994) Photoinhibition and the light environment within leaves. In: Baker NR, Bowyer JR (eds) Photoinhibition of photosynthesis. BIOS Scientific, Oxford, pp 221–237
Osmond CB, Ramus J, Levavasseur G, Franklin L, Henley WJ (1993) Fluorescence quenching during photosynthesis and photoinhibition of Ulva rotundata Blid. Planta 190: 91–106
Sauvageau C (1918) Recherches sur les laminaires des côtes de France. Mém Acad Sci Inst Fr 56: 1–240
Schreiber U, Bilger W, Neubauer C (1994) Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze ED, Caldwell MM (eds) Ecophysiology of photosynthesis. Springer, Berlin, pp 49–70
Sivalingham PM, Nisizawa K (1990) Ozone hole and its correlation with the characteristic UV-absorbing substance in marine algae. Jap J Phycol 38:365–370
Smith RC, Prézelin BB, Baker KS, Bidigare RR, Boucher NP, Coley T, Karentz D, MacIntyre S, Matlick HA, Menzies D, Ondrusek M, Wan Z, Waters KJ (1992) Ozone depletion: ultraviolet radiation and phytoplankton biology in Antarctic waters. Science, NY 255:952–959
Starr RC, Zeikus JA (1993) UTEX — the culture collection of algae at the University of Texas at Austin. J Phycol 29 (Suppl): 1–106
Tevini M, Grusemann P, Fieser G (1988) Assessment of UV-B stress by chlorophyll fluorescence analysis. In: Lichtenthaler HK (ed) Applications of chlorophyll fluorescence. Kluwer Academic, Amsterdam, pp 229–238
Uhrmacher S, Hanelt D, Nultsch W (1995) Zeaxanthin content and the degree of photoinhibition are linearly correlated in the brown alga Dictyota dichotoma. Mar Biol 123:159–165
Wood WF (1987) Effect of solar ultra-violet radiation on the kelp Ecklonia radiata. Mar Biol 96:143–150
Author information
Authors and Affiliations
Additional information
Communicated by O. Kinne, Oldendorf/Luhe
Rights and permissions
About this article
Cite this article
Dring, M.J., Makarov, V., Schoschina, E. et al. Influence of ultraviolet-radiation on chlorophyll fluorescence and growth in different life-history stages of three species of Laminaria (phaeophyta). Marine Biology 126, 183–191 (1996). https://doi.org/10.1007/BF00347443
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00347443