Abstract
Bioavailability of oxygen for the saline-water invertebrate Artemia franciscana was studied, since both oxygen concentration and oxygen diffusion rate change with salinity. Total haemoglobin concentration and the relative contribution of each of three haemoglobins was measured in specimens acclimated to different salinities and oxygen concentrations. Both haemoglobin concentration and contribution were influenced by salinity and the group observed (males, females with and females without eggs). Multiple regression analysis showed that total haemoglobin concentration was better correlated with oxygen concentration than oxygen partial pressure. The relative proportions of haemoglobins 1, 2 and 3 were better correlated with oxygen partial pressure than oxygen concentration. These results are related to the oxygen carrying capacity and oxygen affinity of the haemolymph. Our results show that oxygen bioavailability in a saline-water environment depends on the response (relative contribution of Hb1, Hb2 and Hb3 or total haemoglobin concentration) that is studied. They also show that oxygen concentration and oxygen partial pressure have a different physiological impact on brine shrimp.
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Blakesley, R. W., Boezi, J. A. (1977). A new staining technique for proteins in polyacrylamide gels using coomassie brilliant blue g250. Analyt. Biochem. 82: 580–582
Blust, R., Van der Lidden, A., Verheyen, E., Decleir, W. (1988). Evaluation of microwave heating digestion and graphite furnace atomic absorption spectrometry with continuum source background correction for the determination of iron, copper and cadmium in brine shrimp. J. Analyt. atom. Spectrom. 3: 387–393
Bowen, S. T., Lebherz, H. G., Poon, M.-C., Chow, V. H. S., Grigliatti, T. A. (1969). The hemoglobins of Artemia salina—I. Determination of phenotype by genotype and environment. Comp. Biochem. Physiol. 31: 733–747
Croghan, P. C. (1958). The osmotic and ionic regulation of Artemia salina (L.). J. exp. Biol. 35: 219–233
De Wachter, B., Van den Abbeele, J. (1991). The influence of acclimation on salinity and oxygen on the respiration of the brine shrimp, Artemia franciscana. Comp. Biochem. Physiol. 98A(2): 293–298
De Wachter, B., Van Den Abbeele, J., Wolf, G. (submitted). Effects of oxygen and salinity on the oxygen binding of haemoglobins in the brine shrimp Artemia franciscana. Physiol. Zoöl.
Dutrieu, J. (1960). Observations biochimiques et physiologiques, sur le developpement d'Artemia salina Leach. Archs Zool. exp. gén. 99: 1–134
Ferry, J. A., Nichols, R. C., Condon, S. J., Stubbs, J. D., Bowen, S. T. (1983). Artemia hemoglobins. Increase in net synthesis of the b-polypeptide (relative to the a-polypeptide) in hypoxia. Biochim. biophys. Acta 739: 249–257
Gilchrist, B. M. (1955). Haemoglobin in Artemia. Proc. R. Soc. (Ser. B.) 143: 136–146
Glantz, S. A., Slinker, B. K. (1990). Primer of applied regression and analysis of variance. McGraw-Hill, Singapore
Heip, J., Moens, L., Hertsens, R., Wood, E. J., Heyligen, H., Van Broeckhoven, A., Vrints, R., De Chaffy, D., Kondo, M. (1980). Artemia extracellular hemoglobins: ontogeny, structure and in vivo radiolabelling. In: Persoone, G., Sorgeloos, P., Roels, O., Jaspers, E. (eds.) The brine shrimp Artemia, Vol. 2, Physiology, biochemistry, molecular biology. Universa Press, Wetteren, p. 427–448
Heip, J., Moens, L., Joniau, M., Manatosh, K. (1978a). Ontogenetical studies extracellular hemoglobins of Artemia salina. Devl Biol. 64: 73–81
Heip, J., Moens, L., Kondo, M. (1978b). Effect of concentrations of salt and oxygen on the synthesis of extracellular hemoglobins during development of Artemia salina. Devl Biol. 63: 247–251
Lange, R., Staaland, H., Mostad, A. (1972). The effect of salinity and temperature on solubility of oxygen and respiratory rate in oxygen-dependent marine invertebrates. J. exp. mar. Biol. Ecol. 9: 217–229
Mitchell, B. D., Geddes, M. C. (1977). Distribution of the brine shrimps Parartemia zietziana SAYCE and Artemia salina (L.) along a salinity and oxygen gradient in a South Australian saltfield. Freshwat. Biol. 7: 461–467
Moens, L., Geelen, D., Van Hauwaert, M.-L., Wolf, G., Blust, R., Witters, R., Lontie, R. (1984). The structure of Artemia sp. haemoglobin—cleavage of the native molecules into functional units by limited subtilisin digestion. Biochem. J. 223: 861–869
Moens, L., Kondo, M. (1976). The structure of Artemia salina haemoglobins. Eur. J. Biochem. 67: 397–402
Morris, J. E., Afzelius, B. A. (1967). The structure of the shell and outer membranes in encysted Artemia embryos during cryptobiosis and development. J. Ultrastruct. Res. 20: 244–259
Sokal, R. R., Rohlf, F. J. (1981). Biometry. The principles and practice of statistics in biological research, 2nd edn. W.H. Freeman & Co., San Francisco
Van Den Branden, C., D'Hondt, J., Moens, L., Decleir, W. (1978). Functional properties of the hemoglobins of Artemia salina L. Comp. Biochem. Physiol. 60A(2): 185–187
Vos, J., Bernaerts, F., Gabriels, I., Decleir, W. (1979). Aerobic and anaerobic respiration of adult Artemia salina L., acclimated to different oxygen concentrations. Comp. Biochem. Physiol. 62A: 545–548
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Communicated by O. Kinne, Oldendorf/Luhe
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De Wachter, B., Blust, R. & Decleir, W. Oxygen bioavailability and haemoglobins in the brine shrimp Artemia franciscana . Marine Biology 113, 193–200 (1992). https://doi.org/10.1007/BF00347271
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DOI: https://doi.org/10.1007/BF00347271