Summary
A new type of chromosomal rearrangements associated with a transposable element has been described for IS4. These rearrangements are deletions, in which the transposable element IS4 and DNA adjacent to it on either side is lost. These deletions are at least ten times more frequent in the presence of IS4, than when the element is absent from that region. The formation of bidirectional deletions is more than 1,000 times more frequent than precise excision of IS4.
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Benett, PM, Grinsted J, Richmond MH (1977) Transposition of TnA does not generate deletions. Mol Gen Genet 154:205
Calos MP, Miller JH (1980) Transposable Elements. Cell 20:579
Chaing SJ, Clowes RC (1980) Intramolecular transposition and inversion in plasmid R6K. J Bacteriol 142:668
Chow LT, Davidson N, Berg D (1974) Electronmicroscopic study of the structures of λdv DNAs. J Mol Biol 86:69
Clements MB, Syvanen M (1981) Isolation of a polA mutation that affects transposition of insertion sequences and transposons. Cold Spring Harbor Symp Quant Biol 45:201
Cornelis G, Saedler H (1980) Deletions and an inversion induced by a resident IS1 of the Lactose transposon Tn 951. Mol Gen Genet 178:367
Davis RW, Simon M, Davidson N (1971) Electronmicroscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Grossman L, Moldave K (eds) Methods of enzymology, vol XXI. Academic Press, New York
Faelen M, Toussaint A (1978) Stimulation of deletions in the E. coli chromosome by partially induced Mu cts 62 prophages. J Bacteriol 136:477
Fennewald MA, Shapiro JA (1979) Transposition of Tn7 in Pseudomonas aeruginosa and isolation of Alk-Tn7 mutations. J Bacteriol 139:264
Foster TJ, Lumblad V, Hanly-Way S, Halling S, Kleckner N (1981) Three Tnϕ-associated excision events: Relationship to transposition and role of direct and inverted repeats. Cell 23:215
Galas DJ, Chandler M (1981) On the molecular mechanisms of transposition. Proc Nat Acad Sci USA 78:4858
Grunstein M, Hogness DS (1975) Colony hybridization: A method for isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961
Habermann P, Klaer S, Kühn S, Starlinger P (1979) Is4 is found between eleven or twelve base pair duplications. Mol Gen Genet 175:369
Khatoon H, Bukhari AI (1981) DNA rearrangements associated with reversion of bacteriophage Mu induced mutations. Genetics 98:1
Klaer R, Pfeifer D, Starlinger P (1980) IS4 is still found at its chromosomal site after transposition to galT. Mol Gen Genet 178:281
Kleckner N (1981) Transposable elements in prokaryotes. Annu Rev Genet 15:341–405
Kleckner N, Reichardt K, Botstein D (1979) Inversions, and deletions of the Salmonella chromosome generated by the translocatable tetracycline-resistance element Tnϕ. J Mol Biol 127:89
Kleckner N, Ross DG (1980) A recA-dependent genetic switch generated by transposon Tnϕ. J Mol Biol 144:215
Ljungquist E, Bukhari AI (1977) State of prophage Mu DNA upon induction. Proc Natl Acad Sci USA 74:3143
Lusky M, Kröger M, Hobom G (1981) Detection of replicational inceptor signals in IS5. Cold Spring Harbor Symp Quant. Bio 45:373
Maniatis T, Jeffrey A, Kleid OG (1975) Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci USA 72:1184
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory. New York
Monk M, Kinross J (1972) Conditional lethality of recA and recB derivates of a strain of E. coli K12 with a temperative-sensitive DNA Polymerase I. J Bacteriol 109:97
Ohtsubo H, Ohtsubo E (1978) Nucleotide sequence of an insertion element, Is1. Proc Natl Acad Sci USA 75:615–619
Pfeifer D, Oellermann R (1967) Mapping of the gal mutants by transducing λdgal phages carrying deletions of the gal region. Mol Gen Genet 99:248
Reif HJ, Saedler H (1975) IS1 is involved in deletion formation in the gal region of E. coli K12. Mol Gen Genet 137:17
Saedler H, Cornelis J, Cullum J, Schumacher B, Sommer H (1981) Is1-mediated DNA rearrangements. Cold Spring Harbor Symp Quant Biol 45:93
Sasakawa C, Uno R, Yoshikawa M (1981) The requirement for both DNA polymerase and 5′ to 3′ exonuclease activities of DNA polymerase I during Tn5 transposition. Mol Gen Genet 182:19
Sommer H, Schumacher B, Saedler H (1981) A new type of IS1-mediated deletion. Mol Gen Genet 184:300
Starlinger P (1980) IS elements and transposons. Plasmid 3:241
Szybalski, EH, Szybalski W (1979) A comprehensive molecular map of bacteriophage lambda. Gene 7:219
Toussaint A, Faelen M, Bukhari AI (1977) Mu-mediated illegitimate recombination as an integral part of Mu life cycle. In: Bukhari A, Shapiro J, Aclhya, S (eds) Insertion, elements, plasmids and episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 275–285
Trinks K, Habermann P, Beyreuther K, Starlinger P, Ehring R (1981) An IS4-encoded protein is synthesized in minicells. Mol Gen Genet 182:183
Watson G, Paigen K (1981) Isolation and characterization of an E. coli bacteriophage requiring cellwall galactose. J Virol 8:669
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Communicated by H. Saedler
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Habermann, P., Starlinger, P. Bidirectional deletions associated with IS4. Molec. Gen. Genet. 185, 216–222 (1982). https://doi.org/10.1007/BF00330790
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DOI: https://doi.org/10.1007/BF00330790