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Development of a procedure for the determination of chromium in samples of urine and serum by neutron activation analysis

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Summary

Since the mid-fifties the possibility of a causal relationship between deficient chromium and insulin metabolism and the manifestation of certain varieties of diabetes mellitus has been presumed. The determination of the chromium status under pathophysiological conditions may be helpful for the study of this problem. For these purposes an analytical procedure as reference system was developed which allows the determination of chromium in biological matrices down to the concentration of 0.03 ng/ml. It is based on NAA and is used in the framework of a commonly used procedure based on GF-AAS. For its application blood and urine samples are freeze-dried and irradiated. After wet digestion with HNO3 in a microwave combustion system chromium is separated for measurement from the matrix nuclides with the help of the ion-exchanger Cellex-P. The individual steps of the procedure were evaluated by means of tracer experiments.

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References

  1. Mertz W, Schwarz K (1954) Fed Proc 13:469

    Google Scholar 

  2. Mertz W, Schwarz K (1955) Arch Biochem Biophys 58:504

    Google Scholar 

  3. Mertz W, Schwarz K (1959) Am J Physiol 196:614

    Google Scholar 

  4. Versieck J, Hoste J, Barbier F, Steyaert H, De Rudder H, Michels H (1978) Clin Chem 24:303

    Google Scholar 

  5. Greenberg RR, Zeisler R (1988) J Radioanal Nucl Chem 124:5

    Google Scholar 

  6. Steela R, Genova N, Di Casa M, Gallorini M, Orvini E (1976) J Radioanal Chem 34:59

    Google Scholar 

  7. Brajter K, Miazek I (1983) Fresenius Z Anal Chem 315:121

    Google Scholar 

  8. Brajter K, Ciborowaska U, Miazek I (1983) Fresenius Z Anal Chem 315:126

    Google Scholar 

  9. Versieck J, Barbier F, Cornelis R, Hoste J (1982) Talanta 29:973

    Google Scholar 

  10. Büttner I, Hamm V, Knöchel A (1993) Fresenius Z Anal Chem (in preparation)

  11. Doerffel K (1962) Fresenius Z Anal Chem 185:1

    Google Scholar 

  12. Behne D (1980) Proceedings of the 1st International Workshop on Trace Element Analytical Chemistry in Medicine and Biology, pp 769

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Authors and Affiliations

  1. Institute for Inorganic and Applied Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, W-2000, Hamburg 13, Germany

    I. Büttner, V. Hamm, A. Knöchel & R. Sen Gupta

Authors
  1. I. Büttner
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  2. V. Hamm
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  3. A. Knöchel
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  4. R. Sen Gupta
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Additional information

Dedicated to Professor Dr. V. Krivan on the occasion of his 60th birthday.

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Büttner, I., Hamm, V., Knöchel, A. et al. Development of a procedure for the determination of chromium in samples of urine and serum by neutron activation analysis. Fresenius J Anal Chem 346, 446–452 (1993). https://doi.org/10.1007/BF00325859

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  • DOI: https://doi.org/10.1007/BF00325859

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