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Peroxidase-dependent fluorescence decrease of carboxyfluorescein and its enhancement by liposome encapsulation

Application to the enzymatic determination of hydrogen peroxide

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  • Pharmaceutical/Biological Analysis
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Abstract

The fluorescence intensity of 5(6)-carboxyfluorescein (CF) was decreased by addition of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The reaction inside a liposome containing CF and HRP on addition of H2O2 was measured fluorometrically after destruction of the liposome with Triton X-100. The reaction efficiency was higher than that without liposome because CF and HRP were concentrated inside the latter. The determination of H2O2 can be performed with a smaller amount of HRP by liposome encapsulation.

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Author notes

  1. Soichiro Yamamura

    Present address: The Biomembrane Institute, 201 Elliott Ave., W, 98109, Seattle, WA, USA

Authors and Affiliations

  1. Osaka National Research Institute, AIST, Midorigaoka, Ikeda, 563, Osaka, Japan

    Yoshiro Tatsu, Soichiro Yamamura, Hitoshi Yamamoto & Susumu Yoshikawa

Authors
  1. Yoshiro Tatsu
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  2. Soichiro Yamamura
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  3. Hitoshi Yamamoto
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  4. Susumu Yoshikawa
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Tatsu, Y., Yamamura, S., Yamamoto, H. et al. Peroxidase-dependent fluorescence decrease of carboxyfluorescein and its enhancement by liposome encapsulation. Fresenius J Anal Chem 351, 782–785 (1995). https://doi.org/10.1007/BF00323636

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  • DOI: https://doi.org/10.1007/BF00323636

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