Abstract
The transferability of chromatographic conditions from TLC precoated plates to HPLC columns have been investigated using 62 different pesticides. For the investigation different stationary phases (silica gel, RP-18, RP-8, Diol, NH2, CN) have been used with standard substances on the one hand and a real sample on the other hand. Rf-values of the pesticides have been converted to k'p, a retention value comparable to HPLC, and have been then compared with k'c values of the HPLC columns with the corresponding chromatographic system. For the evaluation of the results the respective data pairs have been correlated by linear regression. Good results of transfer have been obtained on every stationary phase. In the case of silica gel- and Diol-phases some restrictions have to be made, because of different activity between TLC and HPLC sorbent. In order to show that the transfer of chromatographic conditions is not limited to standards, the experiments have been extended to a spiked real sample (lettuce). The influence of the matrix is shown to be negligible and excellent transfer results are able to be realised.
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Reuke, S., Hauck, H.E. Thin-layer chromatography as a pilot technique for HPLC demonstrated with pesticide samples. Fresenius J Anal Chem 351, 739–744 (1995). https://doi.org/10.1007/BF00323629
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DOI: https://doi.org/10.1007/BF00323629