Summary
Important biological functions and cellular recognition phenomena are supposedly governed by specific sugar-protein interactions. Human hematopoietic cell lines offer an excellent model for the study of the expression of endogenous receptors for the carbohydrate part of glycoconjugates with respect to cell lineage and modulation by differentiation. Initially, a panel of fluorescent (neo)glycoproteins was successfully employed to demonstrate cytologically the actual presence of such receptors on different cell lines: the B lymphoblast line, Daudi; the T cell lymphoblastic leukemia line, P 12; the multipotent leukemic line, K 562 and the promyelocytic line, HL 060. Biochemical analyses were performed using affinity chromatography on supports with immobilized lactose and asialofetuin (simple or complex β-galactosides), melibiose (α-galactoside), fucose, N-acetyl-D-galactosamine, maltose (α-glucoside), the mannose-rich yeast glycoprotein, mannan, glycopeptides containing sialic acid residues and heparin. Subsequently, sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis was used to detect cell lineage-dependent changes in these parameters. Differentiation-dependent changes in the expression of receptors with specificity to galactose, N-acetylgalactosamine, maltose and heparin were similarly uncovered upon dimethyl sulfoxide-induced differentiation of HL 60 cells. Differences in this type of cellular characteristic were also apparent for lymphoma cells from patients with various histological subtypes of lowgrade lymphomas. This initial description of lineage- and differentiation-dependent differences in various human hematopoietic cell lines and in cells from patients with lowgrade lymphomas suggests that advances in the knowledge of the composition of endogenous sugar receptors (lectins) may aid in understanding aspects of the biological behavior of hematopoietic cells and their related malignancies via participation of sugar-protein (lectin) interactions.
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References
Aizawa S, Tavassoli M (1987) In vitro homing of hemopoietic stem cells is mediated by a recognition system with galactosyl and mannosyl specificities. Proc Natl Acad Sci USA 84: 4485–4489
Allen HJ, Kachurek D, Bulera S, Kisailus E, DiCioccio R (1986) In vitro synthesis of carbohydrate-binding proteins by human leucocytes. Immunol Invest 15: 123–138
Blaese RM, Tosato G, Greene WC, Fleisher TA, Muchmore AV (1983) The role of cell surface lectin-carbohydrate interactions in cellular recognition, cooperation and regulation. Am J Pediatr Hematol Oncol 5: 199–206
Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93–99
Boyum A (1976) Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol 5 [Suppl 5]: 9–15
Brenan M, Prish CR (1986) Modification of lymphocyte migration by sulfated polysaccharides. Eur J Immunol 16: 423–430
Carding SR, Thorpe SJ, Thorpe R, Feizi T (1985) Transformation and growth related changes in levels of nuclear and cytoplasmic proteins antigenically related to mammalian β-galactoside-binding lectin. Biochem Biophys Res Commun 127: 680–686
Decker JM (1980) Lectin-like molecules on murine thymocytes and splenocytes. Mol Immunol 17: 803–808
Frazier WA, Glaser L, Gottlieb DI (1982) Cellular recognition. Alan R. Liss, New York
Fuduka M (1985) Cell surface glycoconjugates as oncodifferentiation markers in hematopoietic cells. Biochim Biophys Acta 780: 119–150
Gabius HJ (1987) Endogenous lectins in tumors and the immune system. Cancer Invest 5: 39–46
Gabius HJ (1988) Tumorlektinologie: Ein Gebiet im Schnittpunkt von Zuckerchemie, Biochemie, Zellbiologie und Onkologie. Angew Chem 100: 1321–1330; Tumorlectinology: at the intersection of carbohydrate chemistry, biochemistry, cell biology and oncology. Angew Chem Int Edit Engl 27: 1267–1276
Gabius HJ (1989) Potential participation of tumor lectins in cancer diagnosis, therapy and biology. Adv Lectin Res (in press)
Gabius HJ, Engelhardt R (1988) Sugar receptors of different types in human metastases to lung and liver. Tumor Biol 9: 21–36
Gabius HJ, Nagel GA (1988) Lectins and glycoconjugates in oncology, Springer, Berlin Heidelberg New York Tokyo
Gabius HJ, Vehmeyer K, Engelhardt R, Nagel GA, Cramer F (1985) Carbohydrate-binding proteins of tumor lines with different growth properties. I. Differences in their pattern for three clones of rat fibroblasts transformed with a myeloproliferative sarcoma virus. Cell Tissue Res 241: 9–15
Gabius HJ, Bandlow G, Schirrmacher V, Nagel GA, Vehmeyer K (1986) Differential expression of endogenous sugar-binding proteins (lectins) in murine tumor model systems with metastatic capacity. Int J Cancer 39: 643–648
Gabius HJ, Bodanowitz S, Schauer A (1988a) Endogenous sugar-binding proteins in human breast tissue and benign and malignant breast lesions. Cancer 61: 1125–1131
Gabius HJ, Vehmeyer K, Gabius S, Nagel GA (1988b) Clinical application of various plant and endogenous lectins to leukemia. Blut 56: 147–152
Gahmberg CG, Andersson LC (1982) Surface glycoproteins of malignant human leukocytes. Biochim Biophys Acta 651: 65–83
Hakomori SI, Kannagi R (1983) Glycosphingolipids as tumor-associated and differentiation marker. J Natl Cancer Inst 71: 231–251
Kamenov B, Kieran MW, Barrington-Leigh J, Longenecker BM (1984) Homing receptors as functional markers for classification, prognosis, and therapy of leukemias and lymphomas. Proc Soc Exp Biol Med 177: 211–219
Kieda C, Roche AC, Delmotte F, Monsigny M (1980) Lymphocyte membrane lectins. FEBS Lett 99: 329–332
Liener IE, Sharon N, Goldstein IJ (eds) (1986) The Lectins: Properties, Functions and Applications in Biology and Medicine. Academic Press, Orlando
Monsigny M, Roche AC, Midoux P, Kieda C, Mayer R (1988) Endogenous lectins of myeloid and tumor cells: characterization and biological implications. In: Gabius HJ, Nagel GA (eds) Lectins and glycoconjugates in oncology. Springer, Berlin Heidelberg New York Tokyo, pp 25–48
Ochs D (1983) Protein contaminants of sodium dodecyl sulfate-polyacrylamide gel. Anal Biochem 135: 470–474
Pink JRL (1983) Changes in T-lymphocyte glycoprotein structures associated with differentiation. Contemp Top Mol Immunol 2: 89–113
Reading CL (1984) Carbohydrate structure, biological recognition and immune function. In: Ivatt RJ (ed) The Biology of Glycoproteins. Plenum Press, New York, pp 235–321
Redinbaugh MG, Campbell WH (1985) Adaptation of the dye-binding protein assay to microtiter plates. Anal Biochem 147: 144–147
Roxburgh AE (1988) Oligosaccharide components of surface glycoproteins from leukemic lymphocytes of chronic lymphocytic leukemia and non-Hodgkin's lymphoma. Leuk Res 12: 95–102
Stoolman LM, Rosen SD (1983) Possible role for cell surface carbohydrate-binding molecules in lymphocyte recirculation. J Cell Biol 96: 722–729
Uhlenbruck G (1987) Bacterial lectins: mediators of adhesion. Zentralbl Bakteriol Microbiol Hyg [A] 263: 497–508
Van Beek W, Tulp A, Bolscher J, Blanken G, Roozendaal K, Egbers M (1984) Transient versus permanent expression of cancer-related glycopeptides on normal versus leukemic myeloid cells coinciding with marrow eggress. Blood 63: 170–176
Volman GN, Narasimhan S, Letarte M (1987) Analysis of asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells. Mol Immunol 24: 871–886
Weissman IL (1987) Lymphocyte homing receptors and the immune response in vivo. Bio Essays 5: 112–116
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Dedicated to Prof. G. Uhlenbruck on the occasion of his 60th birthday
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Gabius, S., Hellmann, K.P., Ciesiolka, T. et al. Lineage- and differentiation-dependent alterations in the expression of receptors for glycoconjugates (lectins) in different human hematopoietic cell lines and low grade lymphomas. Blut 59, 165–170 (1989). https://doi.org/10.1007/BF00320062
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DOI: https://doi.org/10.1007/BF00320062