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The first analysed archegoniate mitochondrial gene (COX3) exhibits extraordinary features

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Summary

The first mitochondrial-encoded gene of an archeogoniate has been identified, cloned and sequenced. The cytochrome oxidase III gene (cox3) of the moss Physcomitrella patens consists of a 618 bp open reading frame with high homology (around 72%) to known cox3 sequences of higher plants. Nevertheless, it is a quarter shorter than these. The cox3 gene of P. patens contains no introns and reveals a G+C-content of 41.3%. The region containing the cox3 gene exists as a single copy in the mitochondrial genome as shown by restriction mapping. In the 5′ flanking sequence a putative ribosome binding site and a putative secondary structure were found. Two main transcripts of 2.4 kb and 2.6 kb were detected indicating a complex mitochondrial transcription pattern possibly due to co-transcription. Additional open reading frames were found downstream from, as well as upstream of, the cox3 gene. In Western blots a polyclonal cox3 antibody from yeast detected one single band with an apparent molecular weight of 22 kDa.

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Authors and Affiliations

  1. Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, W-2000, Hamburg 52, Federal Republic of Germany

    Joachim R. Marienfeld, Ralf Reski & Wolfgang O. Abel

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  1. Joachim R. Marienfeld
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  2. Ralf Reski
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  3. Wolfgang O. Abel
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Communicated by K. Esser

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Marienfeld, J.R., Reski, R. & Abel, W.O. The first analysed archegoniate mitochondrial gene (COX3) exhibits extraordinary features. Curr Genet 20, 319–329 (1991). https://doi.org/10.1007/BF00318522

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  • DOI: https://doi.org/10.1007/BF00318522

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