Effect of formalin fixation on the dry mass of isolated rat liver nuclei

Summary

Effect of formalin fixation on the dry mass of isolated rat liver nuclei was studied by interference microscopy. It was found that a fixation time of 2–6 hrs prevents completely the elution of water-extractable material from nuclei. Nuclei unfixed, water-extracted for 4 hrs lose about 20% of their initial dry mass. Fixation time longer than 6 hrs resulted also in a decrease of dry mass of the nuclei.

Liquid scintillation counting of 14C-formaldehyde served for finding the influence of bound formaldehyde on dry mass values. As calculated, after 2 hrs fixation with formalin the bound formaldehyde constitutes 3.6% of the dry mass of isolated untreated nuclei.

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Abramczuk, J. Effect of formalin fixation on the dry mass of isolated rat liver nuclei. Histochemie 29, 207–212 (1972). https://doi.org/10.1007/BF00306144

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Keywords

  • Public Health
  • Formaldehyde
  • Formalin
  • Fixation Time
  • Liquid Scintillation