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Artificial DNA-mediated genetic transformation of the photosynthetic nitrogen-fixing bacterium Rhodospirillum rubrum

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Abstract

Methods were identified for the introduction of plasmid DNA into Rhodospirillum rubrum, including freeze-thaw and CaCl2-based techniques.

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Abbreviations

cfu:

colony forming units

DMSO:

dimethyl sulfoxide

DTT:

dithiothreitol

O.D.680 :

optical density at 680 nm

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Author notes

  1. Wayne P. Fitzmaurice

    Present address: Department of Genetics, North Carolina State University, 27695-7614, Raleigh, NC, USA

Authors and Affiliations

  1. Department of Bacteriology, University of Wisconsin-Madison, 53706, Madison, WI, USA

    Wayne P. Fitzmaurice & Gary P. Roberts

  2. the Center for the Study of Nitrogen Fixation, University of Wisconsin-Madison, 53706, Madison, WI, USA

    Wayne P. Fitzmaurice & Gary P. Roberts

Authors
  1. Wayne P. Fitzmaurice
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  2. Gary P. Roberts
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Fitzmaurice, W.P., Roberts, G.P. Artificial DNA-mediated genetic transformation of the photosynthetic nitrogen-fixing bacterium Rhodospirillum rubrum . Arch. Microbiol. 156, 142–144 (1991). https://doi.org/10.1007/BF00290987

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  • DOI: https://doi.org/10.1007/BF00290987

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