Summary
Serial chromosome studies were performed on four monocytic cell lines established from bone marrow samples of patients suffering from hematopoietic disorders other than leukemia. A spontaneous in vitro transformation towards a malignant phenotype has been found to be related to the karyotype evolution. The correlation between the chromosome changes of these cell lines and those described in human cancer and leukemia is discussed.
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Bennett JM, Catovsky D, Daniel MT, Flandrin G, Galton DAG, Gralnick HR, Sultan C (FAB Co-operative Group) (1976) Proposals for the classification of the acute leukemias. Br J Haematol 33:451–458
Berger R, Bernheim A, Sigaux F, Daniel MT, Valensi F, Flandrin G (1982) Acute monocytic leukemia chromosome studies. Leuk Res 6:17–26
Berger R, Bloomfield CD, Sutherland GR (1985) Report of the committee on chromosome rearrangements in neoplasia and on fragile sites. (8th International Workshop on Human Gene Mapping.) Cytogenet Cell Genet 40:490–535
Fourth International Workshop on Chromosomes in Leukemia, 1982 (1984) Rearrangements of 11q. Cancer Genet Cytogenet 40:294–295
Gahrton G, Robèrt K-H, Friberg K, Zech L, Bird AG (1980) Nonrandom chromosomal aberrations in chronic lymphocvtic leukemia revealed by polyclonal B-cell-mitogen stimulation. Blood 56: 640–647
Griffin FM Jr, Bianco C, Silverstein SC (1975) Characterization of the macrophage receptor for complement and demonstration of its functional independence from the receptor for the Fc portion of immunoglobulin G. J Exp Med 141:1269–1271
Han T, Ozer H, Sadamori N, Emrich L, Gomez GA, Henderson ES, Bloom ML, Sandberg AA (1984) Prognostic importance of cytogenetic abnormalities in patients with chronic lymphocytic leukemia. N Engl J Med 310:288–292
ISCN 1978 (1978) An International System for Human Cytogenetic Nomenclature. Birth Defects 14, No.8; Cytogenet Cell Genet 21:309–404
Mitelman F (1984) Restricted number of chromosomal regions implicated in aetiology of human cancer and leukemia. Nature 310:325–327
Monaco G, Vigneti E, Lancieri M, Cornaglia-Ferraris P, Lambertenghi-Deliliers G, Revoltella RP (1982) Induction of monocytemacrophage differentiation in a new diploid line of human hematopoietic cells (CM-S) by phorbol esters. Cancer Res 42:4182–4189
Pearson MG, Vardiman JW, Le Beau MM, Rowley JD, Scwartz S, Kerman SL, Cohen MM, Fleischman EW, Prigogina EL (1985) Increased numbers of marrow basophils may be associated with a t(6;9) in ANLL. Am J Hematol 18:393–403
Revoltella RP, Butler RH, Musiani P, Piantelli M, Ranelletti FO (1985) Interleukin-1 production by a cloned line of human monocyte-like cells (CM-SM). Scand J Immunol 21:11–20
Revoltella RP, Vigneti E, Park M, Simoni G, Romitti L (1986) Spontaneous in vitro malignant transformation of a monocytic cell line (CM-S) from a patient with congenital hypoplastic anemia. The Cancer J 1:111–117
Rowley JD (1973) Identification of a translocation with quinacrine fluorescence in a patient with acute leukemia. Ann Génét (Paris) 16:109–112
Rowley JD (1983) Consistent chromosome abnormalities in human leukemia and lymphoma. Cancer Invest 1:267–280
Rowley JD, Golomb HM, Dougherty C (1977) 15/17 Translocation, a consistent chromosome change in acute promyelocytic leukemia. Lancet I:549–550
Sambuy Y, Spagnoli L, Vigneti E, Revoltella RP (1985) Ultrastructural studies of spontaneous in vitro transformation of cultured marrow monocyte-macrophage cells from a patient with congenital hypoplastic anemia. Cancer Res 45:1392–1401
Sandberg AA (1980) The chromosomes in human cancer and leukemia. Elsevier, New York
Testa JR (1980) Cytogenetic patterns in polycythemia vera. Cancer Genet Cytogenet 1:207–215
Tiepolo L, Maraschio P, Gimelli G, Cuoco C, Gargani GF, Romano C (1979) Multibranched chromosomes 1, 9 and 16 in a patient with combined IgA and IgE deficiency. Hum Genet 51:127–137
Yam LT, Li CY, Crosby WM (1971) Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol 55:283–296
Yunis JJ (1983) The chromosomal basis of human neoplasia. Science 221:227–236
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Romitti, L., Revoltella, R.P., Vigneti, E. et al. Chromosome changes in human monocytic cell lines with in vitro spontaneous malignant transformation. Hum Genet 74, 256–261 (1986). https://doi.org/10.1007/BF00282544
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DOI: https://doi.org/10.1007/BF00282544