Using various replication mutants of E. coli, the host genes that participate in the replication of some K12-specific single-stranded DNA phages have been determined. Functional products of dnaE,-F,-G and -Z genes are required for the multiplication of ϕK, whereas dnaA,-B,-C(D), H,-I and -P are dispensable for viral replication. In contrast with polB, recA, B, C, or xth functions, host rep activity is essential for ϕK. At the restrictive temperature, the yield of ϕK was markedly reduced in the ligts7 mutant and partially decreased in a polA ts strain. The phage ϕK is thus less dependent on the host cells than ϕX174 and ϕA which require additionally the dnaB,-C(D) and -H functions. Replication of phage St-1 depends on dnaG and -Z gene products, but not on dnaP function. Although not much affected in polA ts host, growth of St-1 was significantly diminished in dnaF or ligts7 mutants.
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Communicated by G. Bertani
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Taketo, A. Host genes involved in the replication of single-stranded DNA phage ϕK. Molec. Gen. Genet. 148, 25–29 (1976). https://doi.org/10.1007/BF00268542
- Gene Product
- Host Cell
- Viral Replication
- Host Gene
- Functional Product