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Localization of ribosomal RNA genes on human acrocentric chromosomes

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Summary

Clonal derivatives of a human heteroploid cell line, with different numbers of acrocentric chromosomes, show different rDNA contents. A linear relationship has been found between the rDNA content and the relative mass of the acrocentric chromosomes (D+G) expressed as the ratio between the mass of their DNA and the mass of the DNA of the whole chromosomal complement. The results suggest that human rRNA genes are located exclusively on the chromosomes of the groups D and G and that all these chromosomes contain rRNA genes.

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References

  • Aloni, Y., Hatlen, L. E., Attardi, G.: Studies of fractionated HeLa cell metaphase chromosomes. J. molec. Biol. 56, 555–563 (1971)

    Google Scholar 

  • Amaldi, F., Attardi, G.: Partial sequence analysis of ribosomal RNA from HeLa cells. J. molec. Biol. 32, 737–755 (1968)

    Google Scholar 

  • Asano, K.: Size heterogeneity of T2 messenger RNA. J. molec. Biol. 14, 71–84 (1965)

    Google Scholar 

  • Attardi, G., Huang, P. C., Kabat, S.: Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system. Proc. nat. Acad. Sci. (Wash.) 54, 185–192 (1965)

    Google Scholar 

  • Birnstiel, M. L., Chipchase, M., Speirs, S.: The ribosomal RNA cistrons. In: Progress in nucleic acid research and molecular biology Davidson, N. J. and Cohn, W. E., eds. vol. 11, p. 351–389. New York: Academic Press 1971

    Google Scholar 

  • Birnstiel, M. L., Grunstein, M.: The ribosomal cistrons of eukariotes. A model system for the study of evolution of serially repeated genes. FEBS Symposium 23, 349–365 (1972)

    Google Scholar 

  • Birnstiel, M. L., Speirs, J., Purdon, I., Jones, K., Loening, U. F.: Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis. Nature (Lond.) 219, 454–463 (1968)

    Google Scholar 

  • Birnstiel, M. L., Wallace, H., Sirlin, J. L., Fischberg, M.: Localization of the ribosomal DNA complements in the nuclear organizer region of Xenopus laevis. Nat. Cancer. Inst. Monogr. 23, 431–448 (1966)

    Google Scholar 

  • Bishop, D. H. L., Robertson, F. W., Burns, J. A., Melli, M.: Methods for the analysis of deoxyribonucleic acid-ribonucleic acid hybridization data. Biochem. J. 115, 361–370 (1969)

    Google Scholar 

  • Brimacombe, R. L. C., Kirby, K. S.: RNA-DNA hybrids by ribosomal RNA from a Rat hepatoma. Biochim. biophys. Acta (Amst.) 157, 362–367 (1968)

    Google Scholar 

  • Bross, K., Krone, W.: On the number of ribosomal RNA genes in man. Hum. Genet. 14, 137–141 (1972)

    Google Scholar 

  • Brown, D. D., Gurdon, J. B.: Absence of ribosomal RNA synthesis in the anucleolate mutant of Xenopus laevis. Proc. nat. Acad. Sci. (Wash.) 51, 139–146 (1964)

    Google Scholar 

  • Brown, D. D., Weber, C. S.: Gene linkage by RNA-DNA hybridization. J. molec. Biol. 34, 681–697 (1968)

    Google Scholar 

  • Darnell, J. E., Jr.: Ribonucleic acids from animal cells. Bact. Rev. 32, 262–290 (1968)

    Google Scholar 

  • Dische, Z.: Color reactions of nucleic acid components. In: The nucleic acids Chargaff, E. and Davidson, J. M., eds. vol. 1, p. 285–305. New York: Academic Press 1955

    Google Scholar 

  • DuPraw, E. J.: Cell and molecular biology. Table 18-1. Quantitative properties of human chromosomes, p. 521. New York: Academic Press 1968

    Google Scholar 

  • Ferguson-Smith, M. A.: The sites of nucleolus formation in human pachytene chromosomes. Cytogenetics 3, 124–134 (1964)

    Google Scholar 

  • Ferguson-Smith, M. A., Handmaker, S. D.: The association of satellited chromosomes with specific chromosomal regions in cultured human somatic cells. Ann. hum. Genet. 27, 143–156 (1963)

    Google Scholar 

  • Galibert, F., Larsen, C. J., Lelong, J. C., Boiron, M.: RNA of low molecular weight in ribosomes of mammalian cells. Nature (Lond.) 207, 1039–1041 (1965)

    Google Scholar 

  • Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol. 12, 829–842 (1965)

    Google Scholar 

  • Henderson, A. S., Warburton, D., Atwood, K. C.: Location of ribosomal DNA in the human chromosome complement. Proc. nat. Acad. Sci. (Wash.) 69, 3394–3398 (1972)

    Google Scholar 

  • Huberman, J. A., Attardi, G.: Studies of fractionated HeLa cell metaphase chromosomes. J. molec. Biol. 29, 487–505 (1967)

    Google Scholar 

  • Janteur Ph., Attardi, G.: Relationship between HeLa cell ribosomal RNA and its precursors by high resolution RNA-DNA hybridization. J. molec. Biol. 45, 305–324 (1969)

    Google Scholar 

  • Knowland, J., Miller, L.: Reduction of ribosomal RNA synthesis and ribosomal RNA genes in a mutant of Xenopus laevis which organizes only a partial nucleolus. J. molec. Biol. 53, 329–338 (1970)

    Google Scholar 

  • Maden, B. E. H.: Ribosome formation in animal cells. Nature (Lond.) 219, 685–689 (1968)

    Google Scholar 

  • Miller, O. L., Beatty, B. R.: Visulization of nucleolar genes. Science 164, 955–957 (1969)

    Google Scholar 

  • Ohno, S., Trujillo, J. M., Kaplan, W. D., Kinosita, R.: Nucleolus organizers in the causation of chromosomal anomalies in man. Lancet 1961 II, 123–125

    Google Scholar 

  • Osawa, S., Sibatani, A.: Chromatographic separation of RNA from DNA on MAK columns. In: Methods in enzymology (Colowick, S. P. and Kaplan, eds. vol. 12, Part A, p. 678–684. New York: Academic Press 1967

    Google Scholar 

  • Perani, A., Parisi, B., De Carli, L., Ciferri, O.: Incorporation of amino acids by a cell-free system prepared from human cells cultured in vitro. Biochim. biophys. Acta (Amst.) 161, 223–231 (1968)

    Google Scholar 

  • Quagliarotti, G., Ritossa, F.: On the arrangement of genes for 28 s and 18 s ribosomal RNA's in Drosophila melanogaster. J. molec. Biol. 36, 57–69 (1968)

    Google Scholar 

  • Ritossa, F., Atwood, K. C., Lindsley, D. L., Spiegelman, S.: On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA. Nat. Canc. Inst. Monogr. 23, 449–472 (1966)

    Google Scholar 

  • Ritossa, F., Spiegelman, S.: Localization of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 53, 737–745 (1965)

    Google Scholar 

  • Sedat, J. W., Kelly, R. B., Sinsheimer, R. L.: Fractionation of nucleic acid on benzoylatednaphthoylated DEAE cellulose. J. molec. Biol. 26, 537–540 (1967)

    Google Scholar 

  • Sedat, J. W., Lyon, A., Sinsheimer, R. L.: Purification of Escherichia coli pulse-labeled RNA by benzoylated DEAE-cellulose chromatography. J. molec. Biol. 44, 415–434 (1969)

    Google Scholar 

  • Simoni, G., De Carli, L.: Heterokaryon formation between alkaline phosphatase variants in a human heteroploid cell line. Europ. J. Cancer 6, 1–5 (1970)

    Google Scholar 

  • Stevenin, J., Samec, J., Jacob, M., Mandel, P.: Determination de la fraction du génome codant pour les RNA ribosomiques et messengers dans le cerveau du rat adulte. J. molec. Biol. 33, 777–793 (1968)

    Google Scholar 

  • Wallace, H., Birnstiel, M. L.: Ribosomal cistrons and the nucleolus organizer. Biochim. biophys. Acta (Amst.) 114, 296–310 (1966)

    Google Scholar 

  • Weinberg, R. A., Penman, S.: Processing of 45 s nucleolar RNA. J. molec. Biol. 47, 169–178 (1970)

    Google Scholar 

  • Yerganian, G.: Chromosomes cytology of medical anomalies. In: Radiation induced chromosome aberration (Wolff, S., ed.), p. 259. New York-London: Columbia University Press 1963

    Google Scholar 

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Authors and Affiliations

  1. Laboratorio di Genetica Biochimica ed Evoluzionistica, C.N.R., Pavia, Italy

    S. Spadari, R. Di Lernia, G. Simoni, G. Pedrali-Noy & L. De Carli

  2. Istituto di Genetica, Università di Pavia, Pavia, Italy

    S. Spadari, R. Di Lernia, G. Simoni, G. Pedrali-Noy & L. De Carli

  3. Istituto di Biologia Generale Faculty of Medicine, University of Milan, Milan, Italy

    R. Di Lernia, G. Simoni & L. De Carli

Authors
  1. S. Spadari
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  2. R. Di Lernia
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  3. G. Simoni
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  4. G. Pedrali-Noy
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  5. L. De Carli
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Additional information

A preliminary report of this work was given at the 4th Int. Congress of Human Genetics, Paris 1971 and the abstract was reported in Excerta Medica Int. Cong. Ser. 233, abstract 618.

Communicated by G. Magni

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Spadari, S., Di Lernia, R., Simoni, G. et al. Localization of ribosomal RNA genes on human acrocentric chromosomes. Molec. Gen. Genet. 127, 57–67 (1973). https://doi.org/10.1007/BF00267783

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  • DOI: https://doi.org/10.1007/BF00267783

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