The gene for Klebsiella bacteriophage K11 RNA polymerase: Sequence and comparison with the homologous genes of phages T7, T3, and SP6

Summary

We determined the nucleotide sequence of gene 1 of Klebsiella phage K11, which is a member of the T7 group of phages. The largest open reading frame corresponds to a polypeptide with 906 amino acids and a molecular weight of 100383 daltons. The deduced amino acid sequence of this polypeptide shows 71% homology to the T7 RNA polymerase (the product of T7 gene 1), 72% homology to the T3 RNA polymerase and 27% homology to the SP6 RNA polymerase. Divergent evolution was clearly most pronounced in the amino-terminal portion.

This is a preview of subscription content, access via your institution.

References

  1. Botstein D (1980) A theory of modular evolution for bacteriophage. Ann N Y Acad Sci 254:484–490

    Google Scholar 

  2. Britten RJ, Kohne DE (1968) Repeated sequences in DNA. Science 161:529–540

    CAS  PubMed  Google Scholar 

  3. Buvinger WE, Riley M (1985) Nucleotide sequence of Klebsiella preumoniae lac genes. J Bacteriol 163:850–857

    Google Scholar 

  4. Dayhoff MO, Barker WC, Hunt LT (1983) Establishing homologies in protein sequences. Methods Enzymol 91:524–545

    Google Scholar 

  5. Dietz A, Kössel H, Hausmann R (1985) On the evolution of the terminal redundancies of Klebsiella phage No. 11 and of coliphages T3 and T7. J Gen Virol 66:181–186

    Google Scholar 

  6. Dietz A, Andrejauskas E, Messerschmid M, Hausmann R (1986) Two groups of capsule-specific coliphages coding for RNA polymerases with new promotor specificities. J Gen Virol 67:831–838

    Google Scholar 

  7. Dunn JJ, Studier FW (1983) The complete nucleotide sequence of bacteriophage T7 dNA and the locations of T7 genetic elements. J Mol Biol 166:477–535

    Google Scholar 

  8. Grantham R (1974) Amino acid difference formula to help explain protein evolution. Science 185:862–864

    Google Scholar 

  9. Hausmann R (1988) The T7 group. In: Calendar R (ed) The bacteriophages, vol I. Plenum Publishing, New York, pp 259–289

    Google Scholar 

  10. Korsten KH, Tomkiewicz C, Hausmann R (1979) The strategy of infection as a criterion for phylogenetic relationships of noncoliphages morphologically similar to phage T7. J Gen Virol 43:57–73

    Google Scholar 

  11. Kotani H, Ishizaki Y, Hiraoka N, Obayashi A (1987) Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase. Nucleic Acids Res 15:2653–2664

    Google Scholar 

  12. Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base specific chemical cleavages. Methods Enzymol 65:459–460

    Google Scholar 

  13. McGraw NJ, Bailey JN, Cleaves GR, Dembinski DR, Gocke CR, Joliffe LK, MacWright RS, McAllister WT (1985) Sequence and analysis of the gene for bacteriophage T3 RNA polymerase. Nucleic Acids Res 13:6753–6766

    Google Scholar 

  14. Messing J, Vieira J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276

    Google Scholar 

  15. Moffatt BA, Dunn JJ, Studier FW (1984) Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase. J Mol Biol 173:265–269

    Google Scholar 

  16. Rudolph C, Freund-Mölbert E, Stirm S (1975) Fragments of Klebsiella bacteriophage No. 11. Virology 64:236–246

    Google Scholar 

  17. Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol 143:161–178

    Google Scholar 

  18. Shapiro HA (1970) Distribution of purines and pyrimidines in nucleic acids. In: Sober HA (ed) Handbook of biochemistry: selected data for molecular biology, 2nd ed. CRC Press, West Palm Beach, Fla, pp H83-H85

    Google Scholar 

  19. Studier FW, Dunn JJ (1983) Organization and expression of bacteriophage T7 DNA. Cold Spring Harbor Symp Quant Biol 47:999–1107

    Google Scholar 

  20. Vieira J, Messing J (1982) The pUC plasmids, an M13 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Rudolf Hausmann.

Additional information

Communicated by J. Lengeler

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dietz, A., Weisser, HJ., Kössel, H. et al. The gene for Klebsiella bacteriophage K11 RNA polymerase: Sequence and comparison with the homologous genes of phages T7, T3, and SP6. Molec. Gen. Genet. 221, 283–286 (1990). https://doi.org/10.1007/BF00261733

Download citation

Key words

  • Phage RNA polymerase
  • Klebsiella bacteriophage K11
  • Phage T7 group