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Immunohistochemical demonstration of tumor-associated antigens with the aid of monoclonal and polyclonal antisera in carcinoma of the bladder

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Summary

Keratin was identified with the aid of polyclonal antisera in the cytoplasm in over 90% of the transitional cell carcinomas investigated. The intensity of staining increased with the degree of dedifferentiation. Detection of cytokeratin with monoclonal antibodies was successful in over 80% of samples. All squamous cell carcinomas of the bladder were strongly positive for keratin and cytokeratin. CEA was found in 20% of the G1 and 40% of the G2 and G3 carcinomas of the bladder. Both the prostatic epithelium markers PSA and PAP and the monoclonal antibody Ca1 were negative in all cases.

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Authors and Affiliations

  1. Institute of Pathology of the Free University Berlin, Charlottenburg Medical Center, Berlin, Federal Republic of Germany

    J. Steffens, W. Friedmann & H. Lobeck

  2. Kapellenstraße 68, D-5100, Aachen, FRG

    J. Steffens

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  1. J. Steffens
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  2. W. Friedmann
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  3. H. Lobeck
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Steffens, J., Friedmann, W. & Lobeck, H. Immunohistochemical demonstration of tumor-associated antigens with the aid of monoclonal and polyclonal antisera in carcinoma of the bladder. Urol. Res. 13, 55–59 (1985). https://doi.org/10.1007/BF00261566

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