A tissue culture procedure is described which yields a high percentage of successful longterm cultures of epithelial cells derived from malignant and non-malignant human urogenital tissues.
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Bregman, R. U., Bregman, E. T.: Tissue culture of benign and malignant human genitourinary tumors. J. Urol. 86, 642–649 (1961)
Bubenik, J., Barešová M., Viklický, V., Jakoubková, J., Sainerová, H., Donner, J.: Established cell line of urinary bladder carcinoma (T24) containing tumor-specific antigen. Int. J. Cancer 11, 765–773 (1973)
Fraley, E. E., Ecker, S., Vincent, M. M.: Spontaneous in vitro neoplastic transformation of adult human prostatic epithelium. Science 170, 540–542 (1970)
Fraley, E. E., Elliott, A. Y., Castro, A. E., Cleveland, P., Hakala, T., Stein, N.: Ribonucleic acid virus associated with human urothelial tumors: Significance for diagnosis and treatment. J. Urol. 111, 378–381 (1974)
Hicks, R. M.: The fine structure of the transitional epithelium of rat ureter. J. Cell. Biol. 26, 25–48 (1965)
Hosick, H. L.: A note on growth patterns of epithelial tumor cells in primary culture. Cancer Res. 34, 259–261 (1974)
Koss, L. G.: The asymmetric unit membranes of the epithelium of the urinary bladder of the rat. An electron microscopic study of the Mechanism of maturation and function. Lab. Invest. 21, 154–168 (1969)
Paulson, D.F., Bonar, R.A., Mickey, D.D., Vergara, J., Stone, K.R., Glenn, J.F.: Biology of transitional malignancy. Trans. of the G-U Surg. (in press)
Peterson, L. J., Paulson, D. F., Bonar, R. A.: Response of human urothelium to chemical carcinogens in vitro. J. Urol. 111, 154–159 (1974)
Porter, K. R., Kenyon, K., Badenhausen, S.: Specializations of the unit membrane. Protoplasma 63, 262–274 (1967)
Rigby, C. C., Franks, L. M.: A human tissue culture cell line from a transitional cell tumor of the urinary bladder: Growth, chromosome pattern and ultrastructure. Brit. J. Cancer 24, 746–754 (1970)
Stonington, O. G., Hemmingsen, H.: Culture of Cells as a monolayer derived from the epithelium of the human prostate: A new cell growth technique. J. Urol. 106, 393–400 (1971)
Yajima, T.: Monolayer culture of human urinary bladder tumors. Jap. J. Urol. 59, 128–134 (1968)
Supported by N.I.H. Grant No. 1 R26 CA15972-01 and Veterans Administration Projects 1044 and 7080.
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Stone, K.R., Paulson, D.F., Bonar, R.A. et al. In vitro culture of epithelial cells derived from urogenital tissues. Urol. Res. 2, 149–153 (1975). https://doi.org/10.1007/BF00257263
- Tissue culture