Skip to main content
Log in

Aspartate aminotransferase allozyme variation in a germplasm collection of the domesticated lentil (Lens culinaris)

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Variation at a polymorphic Aspartate aminotransferase locus was assayed in a sample of 298 accessions from the ICARDA germplasm collection of the domesticated lentil (Lens culinaris). Two alleles Aat-1 F and Aat-1 S were detected with global frequencies of 0.51 and 0.49, respectively. Fifty-nine percent of accessions were polymorphic for both alleles. The frequency of outcrossing was estimated from the observed heterozygosity to be about 1%. This is higher than direct estimates of outcrossing and implicates selection in favour of heterozygous gene combinations. Significant variation in allele frequency and in the occurrence of polymorphic accessions was observed between countries or geographic areas. Significant associations were observed between the allozymes and agronomic characters. In particular high frequency of Aat-1 F appeared to be associated with late flowering and maturity and low yield.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allard RW, Kahler AL, Weir BS (1972) The effect of selection on esterase allozymes in a barley population. Genetics 72:489–503

    Google Scholar 

  • Arus P, Tanksley SD, Orton JJ, Jones RA (1982) Electrophoretic variation as tool for determining seed purity and for breeding hybrid varieties of Brassica oleracea. Euphytica 31:417–428

    Google Scholar 

  • Ashton GC, Braden AWH (1961) Serum β-globin polymorphism in mice. Aust J Biol Sci 14:248–253

    Google Scholar 

  • Barulina H (1930) Lentils of the USSR and other countries. Bul Appl Bot Gen Plant Breed (Suppl) [Leningrad, English summary] 40:265–304

    Google Scholar 

  • Brown AHD (1970) The estimation of Wright's fixation index from genotypic frequencies. Genetica 41:399–406

    Google Scholar 

  • Brown AHD (1979) Enzyme polymorphism in plant populations. Theor Popul Biol 15:1–42

    Google Scholar 

  • Brown AHD, Marshall DR, Albrecht L (1974) The maintenance of alcohol dehydrogenase polymorphism in Bromus mollis L. Aust J Biol Sci 27:545–559

    Google Scholar 

  • Brown AHD, Marshall DR, Munday J (1976) Adaptedness of variants at an alcohol dehydrogenase locus in Bromus mollis. Aust J Biol Sci 29:389–396

    Google Scholar 

  • Brown AHD, Nevo E, Zohary D (1977) Association of alleles at esterase loci in wild barley Hordeum spontaneum. Nature 268:430–431

    Google Scholar 

  • Brown AHD, Zohary D, Nevo E (1978) Outcrossing rates and heterozygosity in natural populations of Hordeum spontaneum Koch in Israel. Heredity 41:49–62

    Google Scholar 

  • Cardy BJ, Kannenberg LW (1982) Allozymic variability among maize inbred lines and hybrids: applications for cultivar identification. Crop Sci 22:1016–1020

    Google Scholar 

  • Clegg MT, Allard RW(1972) Patterns of genetic differentiation in the slender wild oat species Avena barbata. Proc Natl Acad Sci USA 69:1820–1824

    Google Scholar 

  • Clegg MT, Allard RW (1973) Viability versus fecundity selection in the slender wild oat Avena barbata. Science 181:667–668

    Google Scholar 

  • Clegg MT, Kahler AL, Allard RW (1978) Estimation of life cycle components of selection in an experimental plant population. Genetics 89:765–792

    Google Scholar 

  • Cubero JI (1981) Origin Taxonomy and Domestication. In: Webb C, Hawtin GC (eds) Lentils. Commonwealth Agricultural Bureaux, England, pp 81–89

    Google Scholar 

  • Ellstrand NC, Torres AM, Levin DA (1978) Density and the rate of apparent outcrossing in Helianthus annum (Asteraceae). Syst Bot 4:403–407

    Google Scholar 

  • Falconer DS (1981) Introduction to quantitative genetics, 2nd edn. Longman, New York

    Google Scholar 

  • Hamrick JL, Allard RW (1975) Correlation between quantitative characters and enzyme genotypes in Avena barbata. Evolution 29:438–442

    Google Scholar 

  • Jain SK (1979) Estimation of outcrossing rates: some alternative procedures. Crop Sci 19:23–26

    Google Scholar 

  • Jain SK, Workman PL (1967) Generalised F-statistics and the theory of inbreeding and selection. Nature 214:674–678

    Google Scholar 

  • Jain SK, Wu L, Vaidya KR (1980) Levels of morphological and allozyme variation in Indian amaranths: a striking contrast. J Hered 71:282–285

    Google Scholar 

  • Jenson RJ, McLeod MJ, Eshbaugh WH, Guttman SI (1979) Numerical taxonomic analyses of allozymic variation in Capsicum (Solanaceae). Taxon 28:315–327

    Google Scholar 

  • Kahler AL, Allard RW (1981) Worldwide patterns of genetic variation among four esterase loci in barley (Hordeum vulgare L.). Theor Appl Genet 59:101–111

    Google Scholar 

  • Marshall DR, Allard RW (1970) Isozyme polymorphisms in natural populations of Avena fatua and A. barbata. Heredity 25:373–382

    Google Scholar 

  • Nevo E, Brown AHD, Zohary D, Storch N, Beiles A (1981) Microgeographic edaphic differentiation in allozyme polymorphisms of wild barley (Hordeum spontaneum, Poaceae). Plant Syst Evol 138:287–292

    Google Scholar 

  • Nygaard DF, Hawtin GC (1981) Production, trade and uses. In: Webb C, Hawtin GC (eds) Lentils. Commonwealth Agricultural Bureaux, England, pp 94–100

    Google Scholar 

  • Quiros CF (1979) Allozyme variation and genetics in the cultivated alfalfa and closely related diploid species. Genetics 91:s100

    Google Scholar 

  • Rasmussen DI (1964) Blood group polymorphism and inbreeding in natural populations of deer mouse Peromyscus maniculatus. Evolution 18:219–229

    Google Scholar 

  • Rick CM, Fobes JF (1975) Allozyme variation in the cultivated tomato and closely related species. Bull Torrey Bot Club 102:376–384

    Google Scholar 

  • Rick CM, Fobes JF, Holle M (1977) Genetic variation in Lycopersicon pimpinellifolium: evidence of evolutionary change in mating systems. Plant Syst Evol 127:139–170

    Google Scholar 

  • Rick CM, Zobel RW, Fobes JF (1974) Four peroxidase loci in red-fruited tomato species: genetics and geographic distribution. Proc Natl Acad Sci USA 71:835–839

    Google Scholar 

  • Shaw CR, Prasad R (1970) Starch gel electrophoresis of enzymes — a compilation of recipes. Biochem Genet 4:297–320

    CAS  PubMed  Google Scholar 

  • Shaw DV, Kahler AL, Allard RW (1981) A multilocus estimator of mating system parameters in plant populations. Proc Natl Acad Sci USA 78:1298–1302

    Google Scholar 

  • Skibinski DOF, Savage FD (1981) Aspartate aminotransferase polymorphism in lentils. Lens 8:21–22

    Google Scholar 

  • Solh M, Erskine W (1981) Genetic resources. In: Webb C, Hawtin GC (eds) Lentils. Commonwealth Agricultural Bureaux, England, pp 101–105

    Google Scholar 

  • Stuber CW, Goodman MM (1983) Inheritance, intracellular localisation and genetic variation of phosphoglucomutase isozymes in maize (Zea mays L.). Biochem Genet 21:667–689

    Google Scholar 

  • Stuber CW, Goodman MM, Moll RH (1982) Improvement of yield and ear number resulting from selection at allozyme loci in a maize population. Crop Sci 22:737

    Google Scholar 

  • Stuber CW, Moll RH, Goodman MM, Schaffer HE, Weir BS (1980) Allozyme frequency changes associated with selection for increased grain yield in maize (Zea mays L.). Genetics 95:225–236

    CAS  Google Scholar 

  • Tanksley SD, Jones RA (1981) Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hortic Sci 16:179–181

    Google Scholar 

  • Wilson VE, Law AG (1972) Natural crossing in Lens esculenta Moench. J Am Soc Hortic Sci 97:142–143

    Google Scholar 

  • Wright S (1951) The genetical structure of populations. Ann Eugen 15:323–354

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by P.M.A. Tigerstedt

Rights and permissions

Reprints and permissions

About this article

Cite this article

Skibinski, D.O.F., Rasool, D. & Erskine, W. Aspartate aminotransferase allozyme variation in a germplasm collection of the domesticated lentil (Lens culinaris). Theoret. Appl. Genetics 68, 441–448 (1984). https://doi.org/10.1007/BF00254816

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00254816

Key words

Navigation