Summary
Variation at a polymorphic Aspartate aminotransferase locus was assayed in a sample of 298 accessions from the ICARDA germplasm collection of the domesticated lentil (Lens culinaris). Two alleles Aat-1 F and Aat-1 S were detected with global frequencies of 0.51 and 0.49, respectively. Fifty-nine percent of accessions were polymorphic for both alleles. The frequency of outcrossing was estimated from the observed heterozygosity to be about 1%. This is higher than direct estimates of outcrossing and implicates selection in favour of heterozygous gene combinations. Significant variation in allele frequency and in the occurrence of polymorphic accessions was observed between countries or geographic areas. Significant associations were observed between the allozymes and agronomic characters. In particular high frequency of Aat-1 F appeared to be associated with late flowering and maturity and low yield.
Similar content being viewed by others
References
Allard RW, Kahler AL, Weir BS (1972) The effect of selection on esterase allozymes in a barley population. Genetics 72:489–503
Arus P, Tanksley SD, Orton JJ, Jones RA (1982) Electrophoretic variation as tool for determining seed purity and for breeding hybrid varieties of Brassica oleracea. Euphytica 31:417–428
Ashton GC, Braden AWH (1961) Serum β-globin polymorphism in mice. Aust J Biol Sci 14:248–253
Barulina H (1930) Lentils of the USSR and other countries. Bul Appl Bot Gen Plant Breed (Suppl) [Leningrad, English summary] 40:265–304
Brown AHD (1970) The estimation of Wright's fixation index from genotypic frequencies. Genetica 41:399–406
Brown AHD (1979) Enzyme polymorphism in plant populations. Theor Popul Biol 15:1–42
Brown AHD, Marshall DR, Albrecht L (1974) The maintenance of alcohol dehydrogenase polymorphism in Bromus mollis L. Aust J Biol Sci 27:545–559
Brown AHD, Marshall DR, Munday J (1976) Adaptedness of variants at an alcohol dehydrogenase locus in Bromus mollis. Aust J Biol Sci 29:389–396
Brown AHD, Nevo E, Zohary D (1977) Association of alleles at esterase loci in wild barley Hordeum spontaneum. Nature 268:430–431
Brown AHD, Zohary D, Nevo E (1978) Outcrossing rates and heterozygosity in natural populations of Hordeum spontaneum Koch in Israel. Heredity 41:49–62
Cardy BJ, Kannenberg LW (1982) Allozymic variability among maize inbred lines and hybrids: applications for cultivar identification. Crop Sci 22:1016–1020
Clegg MT, Allard RW(1972) Patterns of genetic differentiation in the slender wild oat species Avena barbata. Proc Natl Acad Sci USA 69:1820–1824
Clegg MT, Allard RW (1973) Viability versus fecundity selection in the slender wild oat Avena barbata. Science 181:667–668
Clegg MT, Kahler AL, Allard RW (1978) Estimation of life cycle components of selection in an experimental plant population. Genetics 89:765–792
Cubero JI (1981) Origin Taxonomy and Domestication. In: Webb C, Hawtin GC (eds) Lentils. Commonwealth Agricultural Bureaux, England, pp 81–89
Ellstrand NC, Torres AM, Levin DA (1978) Density and the rate of apparent outcrossing in Helianthus annum (Asteraceae). Syst Bot 4:403–407
Falconer DS (1981) Introduction to quantitative genetics, 2nd edn. Longman, New York
Hamrick JL, Allard RW (1975) Correlation between quantitative characters and enzyme genotypes in Avena barbata. Evolution 29:438–442
Jain SK (1979) Estimation of outcrossing rates: some alternative procedures. Crop Sci 19:23–26
Jain SK, Workman PL (1967) Generalised F-statistics and the theory of inbreeding and selection. Nature 214:674–678
Jain SK, Wu L, Vaidya KR (1980) Levels of morphological and allozyme variation in Indian amaranths: a striking contrast. J Hered 71:282–285
Jenson RJ, McLeod MJ, Eshbaugh WH, Guttman SI (1979) Numerical taxonomic analyses of allozymic variation in Capsicum (Solanaceae). Taxon 28:315–327
Kahler AL, Allard RW (1981) Worldwide patterns of genetic variation among four esterase loci in barley (Hordeum vulgare L.). Theor Appl Genet 59:101–111
Marshall DR, Allard RW (1970) Isozyme polymorphisms in natural populations of Avena fatua and A. barbata. Heredity 25:373–382
Nevo E, Brown AHD, Zohary D, Storch N, Beiles A (1981) Microgeographic edaphic differentiation in allozyme polymorphisms of wild barley (Hordeum spontaneum, Poaceae). Plant Syst Evol 138:287–292
Nygaard DF, Hawtin GC (1981) Production, trade and uses. In: Webb C, Hawtin GC (eds) Lentils. Commonwealth Agricultural Bureaux, England, pp 94–100
Quiros CF (1979) Allozyme variation and genetics in the cultivated alfalfa and closely related diploid species. Genetics 91:s100
Rasmussen DI (1964) Blood group polymorphism and inbreeding in natural populations of deer mouse Peromyscus maniculatus. Evolution 18:219–229
Rick CM, Fobes JF (1975) Allozyme variation in the cultivated tomato and closely related species. Bull Torrey Bot Club 102:376–384
Rick CM, Fobes JF, Holle M (1977) Genetic variation in Lycopersicon pimpinellifolium: evidence of evolutionary change in mating systems. Plant Syst Evol 127:139–170
Rick CM, Zobel RW, Fobes JF (1974) Four peroxidase loci in red-fruited tomato species: genetics and geographic distribution. Proc Natl Acad Sci USA 71:835–839
Shaw CR, Prasad R (1970) Starch gel electrophoresis of enzymes — a compilation of recipes. Biochem Genet 4:297–320
Shaw DV, Kahler AL, Allard RW (1981) A multilocus estimator of mating system parameters in plant populations. Proc Natl Acad Sci USA 78:1298–1302
Skibinski DOF, Savage FD (1981) Aspartate aminotransferase polymorphism in lentils. Lens 8:21–22
Solh M, Erskine W (1981) Genetic resources. In: Webb C, Hawtin GC (eds) Lentils. Commonwealth Agricultural Bureaux, England, pp 101–105
Stuber CW, Goodman MM (1983) Inheritance, intracellular localisation and genetic variation of phosphoglucomutase isozymes in maize (Zea mays L.). Biochem Genet 21:667–689
Stuber CW, Goodman MM, Moll RH (1982) Improvement of yield and ear number resulting from selection at allozyme loci in a maize population. Crop Sci 22:737
Stuber CW, Moll RH, Goodman MM, Schaffer HE, Weir BS (1980) Allozyme frequency changes associated with selection for increased grain yield in maize (Zea mays L.). Genetics 95:225–236
Tanksley SD, Jones RA (1981) Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hortic Sci 16:179–181
Wilson VE, Law AG (1972) Natural crossing in Lens esculenta Moench. J Am Soc Hortic Sci 97:142–143
Wright S (1951) The genetical structure of populations. Ann Eugen 15:323–354
Author information
Authors and Affiliations
Additional information
Communicated by P.M.A. Tigerstedt
Rights and permissions
About this article
Cite this article
Skibinski, D.O.F., Rasool, D. & Erskine, W. Aspartate aminotransferase allozyme variation in a germplasm collection of the domesticated lentil (Lens culinaris). Theoret. Appl. Genetics 68, 441–448 (1984). https://doi.org/10.1007/BF00254816
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00254816